DS1 or DS-1 may refer to:
- BOSS DS-1, a guitar distortion pedal
- Digital Signal 1, a T-carrier signaling scheme devised by Bell Labs
- Deep Space 1, a mission to 9969 Braille & 19P/Borrelly
- DS-1 (drug), a selective GABAA α4β3δ agonist drug
- South African Class DS1, a diesel locomotive class
- Datsun DS-1, a car by Nissan, see Datsun DS Series
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