DS3 may refer to:
- Digital Signal 3, a digital signal level 3 T-carrier
- Army Wounded Warrior Program, a program formerly known as Disabled Soldier Support System (DS3)
- DS 3, a supermini car
- Citroën DS3 WRC, a World Rally Car
- DS3 (locomotive), an AC electric locomotive*
- Darkstalkers 3, a 1997 video game
- (29823) 1999 DS3, a main-belt minor planet
- DualShock 3, a gamepad by Sony for the PlayStation 3
- Dungeon Siege III, a 2011 video game
- Dead Space 3, a 2013 video game
- Dark Souls III, a 2016 video game
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