Denisa Chládková (born 8 February 1979) is a former professional tennis player from the Czech Republic.
Chládková did not win any WTA titles, but she is probably best remembered for reaching the Wimbledon quarterfinals in 1997, playing in only her third Grand Slam main draw. In the second round, she stunned Lindsay Davenport for the first top ten win of her career, but eventually lost to the champion Martina Hingis.
On 16 June 2003 she ascended to her career high ranking of No. 31 in the world. That same year she advanced to the fourth round of the Australian Open, her best Grand Slam result since her memorable Wimbledon quarterfinal run six years previously.