She originated the role of Elizabeth I of England in Timothy Findley's play, Elizabeth Rex. She won both an ACTRA Award and a Gemini Award in 2005 for her performance in the play's television adaptation.
She appeared on television and films including such appearances in Alfred Hitchcock Presents, Street Legal, Used People, The Ray Bradbury Theater, Hangin' In, Jane's House, The Long Island Incident, 72 Hours: True Crime, Milk and Honey and Slings and Arrows.
She has done voice-overs in animation including Little Bear, Franklin, Bob and Margaret, Bad Dog, Ultraforce, The Busy World of Richard Scarry, Medabots, Roboroach, Rescue Heroes, Rolie Polie Olie, Freaky Stories, Ned's Newt, Angela Anaconda, Monster Force, Flash Gordon, George Shrinks, Timothy Goes to School, Blaster's Universe, Flying Rhino Junior High, The Adventures of Sam & Max: Freelance Police, Pippi Longstocking, The Dumb Bunnies, Highlander: The Animated Series, World of Quest, The Seventh Portal, Chilly Beach, Wilbur, The Berenstain Bears, Tales from the Cryptkeeper, The Neverending Story, Corduroy, The Dumb Bunnies, Pandalian, Busytown Mysteries, Kaput and Zosky, Jane and the Dragon, The Avengers: United They Stand, Silver Surfer and Cyberchase.
D'Aquila was married to Canadian actor C. David Johnson from 1987 to 1992.
- "Veteran actor Diane D'Aquila sharpens her theatrical edge". The Globe and Mail, September 19, 2011.