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Digitonin CASCC.png
Other names
11024-24-1 YesY
ChemSpider 23753 YesY
ECHA InfoCard 100.031.129
EC Number 234-255-6
Jmol 3D model Interactive image
KEGG C00765 N
PubChem 25444
Molar mass 1229.3 g/mol
Melting point 244.0–248.5 °C (471.2–479.3 °F; 517.1–521.6 K)[1]
-40 ° (Wavlen: 589.3 nm; Temp: 20 °C)[1]
Lethal dose or concentration (LD, LC):
23 mg/kg (rat, intravenous)[2]

4 mg/kg (mouse, intravenous)[3]

Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
N verify (what is YesYN ?)
Infobox references

Digitonin is a steroidal saponin (saraponin) obtained from the foxglove plant Digitalis purpurea. Its aglycone is digitogenin, a spirostan steroid. It has been investigated as a detergent, as it effectively water-solubilizes lipids. As such, it has several potential membrane-related applications in biochemistry, including solubilizing membrane proteins, precipitating cholesterol, and permeabilizing cell membranes.[4][5]

Digitonin is sometimes confused with the cardiac drug digoxin (sometimes also called digitalis or digitoxin) and both can be extracted from the same source.

Structure of Digitonin using sugar abbreviations (Gal = Galactose, Glc = Glucose, Xyl = Xylose)

Physical, chemical and biological properties[edit]

Critical micelle concentration = < 0.5 mM
Average micellar weight = 70000
Appearance = White to off-white powder
Boiling Point/Melting Point = 230 to 240 °C
Aggregation number = 60
Rat-LD50 = 4 mg/kg body weight (IVN), 51 mg/kg body weight (ORAL)


  1. ^ a b Tschesche, R.; Wulff, G. (January 1963). "Über saponine der spirostanolreihe—IX". Tetrahedron (in German). 19 (4): 621–634. doi:10.1016/S0040-4020(01)98548-5. 
  2. ^ Segal, Ruth; Milo-Goldzweig, Ilana; Kaplan, Gideon; Weisenberg, Emil (April 1977). "The protective action of glycyrrhizin against saponin toxicity". Biochemical Pharmacology. 26 (7): 643–645. doi:10.1016/0006-2952(77)90039-9. 
  3. ^ Pitha, Josef; Szente, Lajos (February 1984). "Digitonin derivatives of low toxicity: Potential solubilizers for lipophilic compounds". Journal of Pharmaceutical Sciences. 73 (2): 240–243. doi:10.1002/jps.2600730224. 
  4. ^ Geelen, Math J.H. (December 2005). "The use of digitonin-permeabilized mammalian cells for measuring enzyme activities in the course of studies on lipid metabolism". Analytical Biochemistry. 347 (1): 1–9. doi:10.1016/j.ab.2005.03.032. 
  5. ^ Fiskum, Gary (April 1985). "Intracellular levels and distribution of Ca2+ in digitonin-permeabilized cells". Cell Calcium. 6 (1-2): 25–37. doi:10.1016/0143-4160(85)90032-6.