He graduated in Physics at the University of Rome in 1902. Assistant at the "Regio Ufficio Centrale di Meteorologia e di Geodinamica" (Italian Agency of Meteorology and Geodynamics) from 1905 to 1927. Teacher of Geophysics from 1915 to 1925 at the University of Rome, then professor of Experimental physics at the University of Bari from 1928.
It had been observed that an electroscope in a vessel at earth potential gradually lost its charge, even if very carefully insulated, due to the ionization of the air. Pacini observed simultaneous variations of the rate of ionization on mountains, over a lake, over the sea, and underwater. In an experiment performed in June 1911, Pacini concluded that ionization underwater was significantly lower than on the sea surface. He could then demonstrate that a certain part of the ionization itself must be due to sources other than the radioactivity of the Earth, thus contributing to the discovery of cosmic rays.
- Alessandro De Angelis (2012). L'enigma dei raggi cosmici. Springer.
- Pacini, Domenico (2010). "Penetrating Radiation at the Surface of and in Water". arXiv:1002.1810. Cite journal requires
- Carlson, Per; De Angelis, Alessandro (2011). "Nationalism and internationalism in science: The case of the discovery of cosmic rays". The European Physical Journal H. 35 (4): 309–329. arXiv:1012.5068. doi:10.1140/epjh/e2011-10033-6. S2CID 7635998.