Ehrlich's reagent or Ehrlich reagent is a reagent that contains p-dimethylaminobenzaldehyde (DMAB) and thus can act as an indicator to presumptively identify indoles and urobilinogen. Several Ehrlich tests use the reagent in a medical test; some are drug tests and others contribute to diagnosis of various diseases or adverse drug reactions. A very common Ehrlich test is a simple spot test to identify possible psychoactive compounds such as tryptamines (e.g. DMT) and ergoloids (e.g. LSD). The reagent will also give a positive result for opium, because of the presence of tryptophan in natural opium. It is named after Nobel Prize winner Paul Ehrlich who used it to distinguish typhoid from simple diarrhoea.
The reagent is prepared by dissolving 0.5–2.0 g of p–dimethylaminobenzaldehyde (DMAB) in 50 mL of 95% ethanol and 50 mL of concentrated hydrochloric acid and is best used when fresh. Other alcohols, such as 1-propanol, can also be used as well.
The Ehrlich reagent is similar to a number of other indole tests:
- The van Urk reagent, which uses 0.125 g of p-DMAB, 0.2 mL of ferric chloride solution (25 g/mL) in a solution of 65% sulfuric acid. This is sometimes referred to as the Hofmann reagent or p-DMAB-TS (Test Solution) and gives slightly different colours with different indoles.
- The Renz and Loew reagent, which uses p-dimethylaminocinnamaldehyde and may also be used for the detection of flavonoids.
- The "improved hallucinogen reagent", which uses a 1:1 solution of 5% DMAB in concentrated phosphoric acid (specific gravity 1.45) to methanol.
The Ehrlich reagent works by binding to the C2 position of two indole moieties to form a resonance stabilised carbenium ion compound.
- Pill testing
- Other alkaloid spot tests:
- de Faubert Maunder, MJ (1975). "Field and laboratory test for raw and prepared opium". Bulletin on Narcotics. 27 (1): 71–6. PMID 1039285.
- Spratley, Trinette (2004). "Analytical Profiles for Five "Designer" Tryptamines" (PDF). Microgram Journal. 3 (1–2): 55. Retrieved 2013-10-09.
- O’Neal, Carol L; Crouch, Dennis J; Fatah, Alim A (April 2000). "Validation of twelve chemical spot tests for the detection of drugs of abuse". Forensic Science International. 109 (3): 189–201. doi:10.1016/S0379-0738(99)00235-2. PMID 10725655.
- "Color Test Reagents/Kits for Preliminary Identification of Drugs of Abuse" (PDF). Law Enforcement and Corrections Standards and Testing Program. July 2000. Retrieved 2011-07-24.
- "Ehrlich's Reagent Safety Data Sheet" (PDF). Labchem. 2 July 2014. Retrieved 11 January 2015.
- Ehmann, A. (1977). "The van URK-Salkowski reagent — a sensitive and specific chromogenic reagent for silica gel thin-layer chromatographic detection and identification of indole derivatives" (PDF). Journal of Chromatography A. 132 (2): 267–276. doi:10.1016/S0021-9673(00)89300-0. PMID 188858.
- Sunshine, Irving (1969). Handbook of analytical toxicology. Chemical Rubber Co. p. 408.
p-DMAB-TS: To a cool soln of 65 ml H2S04 in 35 ml H20, add 125 mg para-dimethylaminobenzaldehyde, dissolve, add 1-2 drops of FeCI3-TS.
- "Basic Tests for Pharmaceutical Substances: 5. Reagents". WHO: Essential Medicines and Health Products Information Portal. Retrieved 2019-12-20.
- Maunder, M. J. de Faubert (August 1974). "A field test for hallucinogens: further improvements". Journal of Pharmacy and Pharmacology. 26 (8): 637–638. doi:10.1111/j.2042-7158.1974.tb10677.x. PMID 4155730. S2CID 97915487.
- Kovar, Karl-Artur; Laudszun, Martina (February 1989). "Chemistry and Reaction Mechanisms of Rapid Tests for Drugs of Abuse and Precursors Chemicals" (PDF). UNODC. p. 15. Retrieved 3 January 2016.
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