Enhancer trap

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An enhancer trap is a method in molecular biology that allows hijacking of an enhancer from another gene, and so, identification of enhancers. The enhancer trap construct contains a transposable element and a reporter gene. The first is necessary for (random) insertion in the genome, the latter is necessary for identification of the spatial regulation by the enhancer. On top of this, the construct usually includes a genetic marker, e.g., the white gene producing red-colored eyes in Drosophila, or ampicillin resistance in E. coli.

The most common and basic enhancer traps are: P[lacZ] from the bacterium E. coli and P[GAL4] from yeast. There exists a large number of fly stocks containing GAL4 insertions and an equally large number of fly stocks containing an UAS DNA sequence followed by a gene of interest, which permits the expression of a large number of genes with different GAL4 "drivers". Rather than generating transgenic flies with the enhancer linked directly to the gene of interest (which takes about a year, if you are starting without the appropriate DNA construct), you simply mate (cross) one transgenic fly with another transgenic fly.[1][2][3]

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References[edit]

  1. ^ Andrea Brand and Norbert Perrimon (1993). Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development, 118, pp 401-415.
  2. ^ D. J. Finnegan (1992). Transposable elements. Current Opinion in Genetics and Development, 2, pp 861-867.
  3. ^ J. A. Fischer, E. Giniger, T. Maniatis and M. Ptashne (1988). GAL4 activates transcription in Drosophila. Nature, 332, pp 853-856.