Klooren was born in Tallinn and grew up in Türi. After graduating the Estonian Academy of Music and Theatre in 1968 Klooren worked in the Estonian Drama Theatre. Since 1989 he lived in Järva County where he among other things organized an amateur theatre.
Klooren has also played in several movies including Metskapten (1971), Toomas Nipernaadi (1983), Karoliine hõbelõng (1984), Tallinn pimeduses (1993), Jan Uuspõld läheb Tartusse (2007) and Kormoranid ehk nahkpükse ei pesta (2011).
Enn Klooren's brother Mati Klooren was also an actor.
- Aasaru, Heikki (2011-03-26). "Suri näitleja Enn Klooren". ERR (in Estonian). Retrieved 27 March 2011.
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