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Evolve 4.0 is an open source, freeware artificial life simulator created by Ken Stauffer. In its simulated 2D cellular automata, each cell can behave independently as a unicellular organism or potentially as one cell in a multicellular digital organism, growing, moving, eating, reproducing, and eventually even evolving over time.
Stauffer used the Evolve 4.0 environment in his "One Year of ALife" project, with the goal of running a simulation for an entire year. He finished the project on day 298.