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Fascicle or fasciculus may refer to:
- Muscle fascicle, a bundle of skeletal muscle fibers
- Nerve fascicle, a bundle of axons (nerve fibers)
- Fascicle (botany), a cluster of flowers or leaves, such as the bundles of the thin leaves (or needles) of pines
- A discrete bundle of vascular tissue
- Fasciculus, a fossil comb jelly
- Fasciculus Chemicus, a 17th-century anthology of alchemical writings
- A discrete section of a literary serial issued or published separately
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