The song (its 1975 version) is used in the soundtrack to the 1994 film Pulp Fiction. In the film, Bruce Willis's character sings along to the line, "smoking cigarettes and watching Captain Kangaroo" as he is driving home. In the 1995 film Die Hard with a Vengeance, when Willis's character John McClane is describing his suspension from the police force, he says he was "smoking cigarettes and watching Captain Kangaroo."
In a publicly released video, Dylan Klebold, one of the two teens who committed the Columbine High School Massacre, was filmed by a friend while driving in his car at some point prior to the shooting. They were both listening to "Flowers on the Wall" playing on the radio, singing along to the song and mocking it.
Kurt Vonnegut, Jr. quotes the song's complete lyrics in his 1981 book Palm Sunday calling the song "yet another great contemporary poem by the Statler Brothers" and using it to describe "the present condition" of an American man who had recently departed his family. "It is not a poem of escape or rebirth. It is a poem about the end of a man's usefulness," he adds.
The Muppets have YouTubeviral video parodying the song in which has a band of rats, The Ratler Brothers, sing the song while Beaker struggles with insomnia after being the subject of an experiment involved consuming a large amount of coffee. Considering the considerable childrens' interest in the franchise, the line in the chorus "smoking cigarettes and watching Captain Kangaroo " is changed to other pointless activities.