Franz Hofmeister

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Franz Hofmeister (30 August 1850, in Prague – 26 July 1922, in Würzburg) was an early protein scientist,[1] and is famous for his studies of salts that influence the solubility and conformational stability of proteins. In 1902, Hofmeister became the first to propose that polypeptides were amino acids linked by peptide bonds, although this model of protein primary structure was independently and simultaneously conceived by Emil Fischer.[2][3]

Early life[edit]

Hofmeister's father was a doctor in Prague, where Hofmeister first began his studies, under the physiologist Karl Hugo Huppert, himself a student of Carl Lehmann. Hofmeister's Habilitationsschrift in 1879 concerned the peptic products of digestion.

Hofmeister became a Professor of Pharmacology at the First Faculty of Medicine, Charles University in Prague in 1885, then eventually moved to Strasbourg in 1896.

The Hofmeister series[edit]

Hofmeister discovered a series of salts that have consistent effects on the solubility of proteins and (it was discovered later) on the stability of their secondary and tertiary structure. [4] Anions appear to have a larger effect than cations, and are usually ordered

(This is a partial listing; many more salts have been studied.) The order of cations is usually given as

The mechanism of the Hofmeister series is not entirely clear, but seems to result mainly from effects on the solvent at higher salt concentrations (> 100 mM). Early members of the series increase solvent surface tension and decrease the solubility of nonpolar molecules (salt out); in effect, they strengthen the hydrophobic interaction. By contrast, later salts in the series increase the solubility of nonpolar molecules (salt in) and decrease the order in water; in effect, they weaken the hydrophobic effect. However, these salts also interact directly with proteins (which are charged and have strong dipole moments) and may even bind specifically (e.g., phosphate and sulfate binding to ribonuclease A). Ions that have a strong salting in effect such as I and SCN are strong denaturants, because they salt in the peptide group, and thus interact much more strongly with the unfolded form of a protein than with its native form. Consequently, they pull the unfolding reaction.[5] Moreover, they may have direct interactions with some standard hydrophobic molecules, e.g., benzene. A quantum chemical investigation suggests an electrostatic origin of the Hofmeister series, which appears to quantify this qualitative series (at least for anions). [6]

Protein purification[edit]

The importance of the Hofmeister series to early protein work should not be underestimated, since it provided the chief tool for purifying proteins (sulfate precipitation) over the next ~50 years, one that is still in use today. Hofmeister himself may have been the first to crystallize a protein, hen egg-white albumin. Repeated crystallization was a favourite purification technique in the early days of protein science, and was essential for its development.

Proposal of protein primary structure[edit]

Hofmeister argued for peptide bonds by process of elimination. C-C, ether and ester bonds were unlikely considering the digestion by trypsin. R=C-N-C=R bonds could be eliminated because it would imply a much larger number of carboxylate groups than is observed experimentally.

Hofmeister also argued for peptide bonds based on the biuret reaction observed with all proteins but never with free amino acids. Since biuret has the formula NH2-CO-NH-CO-NH2, that suggested the presence of similar peptide bonds in proteins.

See also[edit]


  1. ^ Fruton, Joseph S. (1985). "Contrasts in Scientific Style. Emil Fischer and Franz Hofmeister: Their Research Groups and Their Theory of Protein Structure". Proceedings of the American Philosophical Society. 129 (4): 313–370. ISSN 0003-049X. JSTOR 986934. PMID 11621201.
  2. ^ "Hofmeister, Franz". Retrieved 4 April 2017.
  3. ^ "Protein, section: Classification of protein". Retrieved 4 April 2017.
  4. ^ Abernethy, John Leo (1967-03-01). "Franz Hofmeister - The impact of his life and research on chemistry". Journal of Chemical Education. 44 (3): 177–80. doi:10.1021/ed044p177. ISSN 0021-9584. PMID 5343300.
  5. ^ Baldwin RL. (1996). "How Hofmeister ion interactions affect protein stability". Biophys J. 71 (4): 2056–63. Bibcode:1996BpJ....71.2056B. doi:10.1016/S0006-3495(96)79404-3. PMC 1233672. PMID 8889180.
  6. ^ Kasimir P. Gregory; Erica J. Wanless; Grant B. Webber; Vince S. J. Craig; Alister J. Page (2021). "The Electrostatic Origins of Specific Ion Effects: Quantifying the Hofmeister Series for Anions". Chem. Sci. doi:10.1039/D1SC03568A.

Further reading[edit]

  • Hofmeister F. (1888) Arch. Exptl. Pathol. Pharmakol., 24, 247.
  • Zhang, Y; Cremer, P (December 2006). "Interactions between macromolecules and ions: The Hofmeister series". Current Opinion in Chemical Biology. 10 (6): 658–63. doi:10.1016/j.cbpa.2006.09.020. PMID 17035073.
  • Zhou, Huan-Xiang (October 2005). "Interactions of macromolecules with salt ions: an electrostatic theory for the Hofmeister effect". Proteins: Structure, Function, and Bioinformatics. 61 (1): 69–78. doi:10.1002/prot.20500. PMID 16044460. S2CID 4996928.
  • Tanford C and Reynolds J. (2001) Nature's robots: a history of proteins, Oxford University Press. ISBN 0-19-850466-7
  • Creighton TE. (1993) Proteins, 2nd ed., W. H. Freeman. ISBN 0-7167-2317-4
  • Jencks WP. (1969) Catalysis in Chemistry and Enzymology, Dover republication (1987). ISBN 0-486-65460-5
  • Collins, KD.; Washabaugh, MW. (1985). "The Hofmeister effect and the behaviour of water at interfaces". Q Rev Biophys. 18 (4): 323–422. doi:10.1017/s0033583500005369. PMID 3916340.
  • John Leo, Abernethy (1967). "Franz Hofmeister - The impact of his life and research on chemistry". Journal of Chemical Education. 44 (3): 177–80. Bibcode:1967JChEd..44..177A. doi:10.1021/ed044p177. PMID 5343300.

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