Acid fuchsin

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Acid fuchsin
Acid fuchsin.svg
IUPAC name
Disodium 2-amino-5-[(Z)-(4-amino-3-sulfonatophenyl)(4-iminio-3-sulfonato-2,5-cyclohexadien-1-ylidene)methyl]-3-methylbenzenesulfonate
3D model (JSmol)
ECHA InfoCard 100.019.833
Molar mass 585.53 g·mol−1
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Infobox references

Acid fuchsin or fuchsine acid is an acidic magenta dye with chemical formula C20H17N3Na2O9S3. It has wide use in histology. It is one of the dyes used in Masson's trichrome stain. This method is commonly used to stain cytoplasm and nuclei of tissue sections in the histology laboratory in order to distinguish muscle from collagen. The muscle stains red with the acid fuchsin, and the collagen is stained green or blue with light green SF yellowish or methyl blue.


  • 1 g of acid fuchsin
  • 1 mL of glacial acetic acid (HOAc)
  • 100 mL of distilled water

See also[edit]


  • Jocelyn H. Bruce-Gregorios, M.D.: Histopathologic Techniques, JMC Press Inc., Quezon City, Philippines, 1974. ISBN 971-11-0853-4