Fura-2-acetoxymethyl ester

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Fura-2-acetoxymethyl ester
Fura 2AM.png
Names
IUPAC name
Acetoxymethyl 2-[5-[bis[(acetoxymethoxy-oxo-

methyl)methyl]amino]-4-[2-[2-[bis[(acetoxymethoxy-oxo- methyl)methyl]amino]-5-methyl-phenoxy]ethoxy]benzofuran-

2-yl]oxazole-5-carboxylate
Other names
Fura-2AM
Identifiers
108964-32-5 YesY
3D model (Jmol) Interactive image
ChemSpider 2610364 YesY
PubChem 3364574
Properties
C44H47N3O24
Molar mass 1001.85
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
YesY verify (what is YesYN ?)
Infobox references

Fura-2-acetoxymethyl ester, often abbreviated Fura-2AM, is a membrane-permeable derivative of the ratiometric calcium indicator Fura-2 used in biochemistry to measure cellular calcium concentrations by fluorescence. When added to cells, Fura-2AM crosses cell membranes and once inside the cell, the acetoxymethyl groups are removed by cellular esterases. Removal of the acetoxymethyl esters regenerates "Fura-2", the pentacarboxylate calcium indicator. Measurement of Ca2+-induced fluorescence at both 340 nm and 380 nm allows for calculation of calcium concentrations based 340/380 ratios. The use of the ratio automatically cancels out certain variables such as local differences in fura-2 concentration or cell thickness that would otherwise lead to artifacts when attempting to image calcium concentrations in cells.

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