Fura-2-acetoxymethyl ester

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Fura-2-acetoxymethyl ester
Fura 2AM.png
Names
IUPAC name
Acetoxymethyl 2-[5-[bis[(acetoxymethoxy-oxo-

methyl)methyl]amino]-4-[2-[2-[bis[(acetoxymethoxy-oxo- methyl)methyl]amino]-5-methyl-phenoxy]ethoxy]benzofuran-

2-yl]oxazole-5-carboxylate
Other names
Fura-2AM
Identifiers
108964-32-5 YesY
ChemSpider 2610364 YesY
Jmol-3D images Image
PubChem 3364574
Properties
C44H47N3O24
Molar mass 1001.85
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Infobox references

Fura-2-acetoxymethyl ester, often abbreviated Fura-2AM, is a membrane-permeable derivative of the ratiometric calcium indicator Fura-2 used in biochemistry to measure cellular calcium concentrations by fluorescence. When added to cells, Fura-2AM crosses cell membranes and once inside the cell, the acetoxymethyl groups are removed by cellular esterases. Removal of the acetoxymethyl esters regenerates "Fura-2", the pentacarboxylate calcium indicator. Measurement of Ca2+-induced fluorescence at both 340 nm and 380 nm allows for calculation of calcium concentrations based 340/380 ratios. The use of the ratio automatically cancels out certain variables such as local differences in fura-2 concentration or cell thickness that would otherwise lead to artifacts when attempting to image calcium concentrations in cells.

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