GABARAP

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GABA(A) receptor-associated protein
Protein GABARAP PDB 1gnu.png
PDB rendering based on 1gnu.
Available structures
PDB Ortholog search: PDBe, RCSB
Identifiers
Symbols GABARAP ; ATG8A; GABARAP-a; MM46
External IDs OMIM605125 MGI1861742 HomoloGene134119 GeneCards: GABARAP Gene
RNA expression pattern
PBB GE GABARAP 200645 at tn.png
More reference expression data
Orthologs
Species Human Mouse
Entrez 11337 56486
Ensembl ENSG00000170296 ENSMUSG00000018567
UniProt O95166 Q9DCD6
RefSeq (mRNA) NM_007278 NM_019749
RefSeq (protein) NP_009209 NP_062723
Location (UCSC) Chr 17:
7.24 – 7.24 Mb
Chr 11:
69.99 – 69.99 Mb
PubMed search [1] [2]

Gamma-aminobutyric acid receptor-associated protein is a protein that in humans is encoded by the GABARAP gene.[1]

Function[edit]

Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. This gene encodes GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton.[2]

A study has screened 2000 participants representing more than 600 families of smokers and examined different genes showing linkage to nicotine addiction. Some of these showing linkage are the GABA-B receptor subunit 2 (GABAB2) gene on chromosome 9, and the GABA-A receptor-associated protein (GABARAP) gene on chromosome 17.[3]

Moreover, GABARAP has a important function in autophagosome mediated autophagy, since it is crucial for autophagosome formation and sequestration of cytosolic cargo into double-membrane vesicles, leading to subsequent degradation after fusion with lysosomes. In addition, GABARAP can mediated selective autophagy because it binds to so-called autophagic receptors (e.g. p62, NBr1), which bind and recruit specific cargo.

Interactions[edit]

GABARAP has been shown to interact with TFRC,[4] ULK1[5][6] and GABRG2.[1][7][8]

References[edit]

  1. ^ a b Wang H, Bedford FK, Brandon NJ, Moss SJ, Olsen RW (Jan 1999). "GABA(A)-receptor-associated protein links GABA(A) receptors and the cytoskeleton". Nature 397 (6714): 69–72. doi:10.1038/16264. PMID 9892355. 
  2. ^ "Entrez Gene: GABARAP GABA(A) receptor-associated protein". 
  3. ^ "Nicotine Addiction Might Be Controlled By Influencing Brain Mechanisms". 2007-12-10. 
  4. ^ Green F, O'Hare T, Blackwell A, Enns CA (May 2002). "Association of human transferrin receptor with GABARAP". FEBS Letters 518 (1-3): 101–106. doi:10.1016/S0014-5793(02)02655-8. PMID 11997026. 
  5. ^ Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931. 
  6. ^ Okazaki N, Yan J, Yuasa S, Ueno T, Kominami E, Masuho Y, Koga H, Muramatsu M (Dec 2000). "Interaction of the Unc-51-like kinase and microtubule-associated protein light chain 3 related proteins in the brain: possible role of vesicular transport in axonal elongation". Brain Research. Molecular Brain Research 85 (1-2): 1–12. doi:10.1016/S0169-328X(00)00218-7. PMID 11146101. 
  7. ^ Nymann-Andersen J, Wang H, Chen L, Kittler JT, Moss SJ, Olsen RW (Mar 2002). "Subunit specificity and interaction domain between GABA(A) receptor-associated protein (GABARAP) and GABA(A) receptors". Journal of Neurochemistry 80 (5): 815–823. doi:10.1046/j.0022-3042.2002.00762.x. PMID 11948245. 
  8. ^ Coyle JE, Qamar S, Rajashankar KR, Nikolov DB (Jan 2002). "Structure of GABARAP in two conformations: implications for GABA(A) receptor localization and tubulin binding". Neuron 33 (1): 63–74. doi:10.1016/S0896-6273(01)00558-X. PMID 11779480. 

Further reading[edit]