GRASP65

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Microinjection of antibodies to GRASP65 prevents normal Golgi stack formation.[1]
GORASP1
PDB 4REY.png
Available structures
PDB Ortholog search: PDBe RCSB
Identifiers
Aliases GORASP1, GOLPH5, GRASP65, P65, golgi reassembly stacking protein 1
External IDs OMIM: 606867 MGI: 1921748 HomoloGene: 49916 GeneCards: GORASP1
RNA expression pattern
PBB GE GORASP1 215749 s at fs.png
More reference expression data
Orthologs
Species Human Mouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_001278789
NM_001278790
NM_031899

NM_028976

RefSeq (protein)

NP_001265718
NP_001265719
NP_114105
NP_114105.1

NP_083252.1
NP_083252

Location (UCSC) Chr 3: 39.1 – 39.11 Mb Chr 9: 119.93 – 119.94 Mb
PubMed search [2] [3]
Wikidata
View/Edit Human View/Edit Mouse

Golgi reassembly-stacking protein 1 (GORASP1) also known as golgi reassembly-stacking protein of 65 kDa (GRASP65) is a protein that in humans is encoded by the GORASP1 gene.[4]

Function[edit]

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The GRASP65 protein is a peripheral membrane protein anchored to the lipid bilayer through myristoylation of a glycine residue near the protein's amino terminus.[5] It is involved in establishing the stacked structure of the Golgi apparatus and linking the stacks into larger ribbons in verterbate cells[5]. It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis.[6][7] GRASP65 can form a complex with the Golgi matrix protein GM130, and this complex binds to the vesicle docking protein p115.[5][a] Several alternatively spliced transcript variants of this gene have been identified, but their full-length natures have not been determined.[4]

Structure[edit]

GRASP65 contains two PDZ domains in the amino-terminal GRASP domain (amino acid residues 2–210), that comprises approximately half of the protein. The GRASP region interacts with the Golgi matrix protein GM130 as well as an intrinsically disordered region in the C-terminus.[5][8]

Interactions[edit]

GORASP1 has been shown to interact with TGF alpha,[9] TMED2[9] and GOLGA2.[9][10][11]

Notes[edit]

  1. ^ This is shown in the external link entitled "Molecular models of GRASP65/GM130/P115-mediated cis-cisternae membrane stacking and vesicle tethering."

References[edit]

  1. ^ Wang Y, Wei JH, Bisel B, Tang D, Seemann J (February 2008). "Golgi cisternal unstacking stimulates COPI vesicle budding and protein transport". Plos One. 3 (2): e1647. doi:10.1371/journal.pone.0001647. PMC 2249924Freely accessible. PMID 18297130. 
  2. ^ "Human PubMed Reference:". 
  3. ^ "Mouse PubMed Reference:". 
  4. ^ a b "Entrez Gene: GORASP1 golgi reassembly stacking protein 1, 65kDa". 
  5. ^ a b c d Hu F, Shi X, Li B, Huang X, Morelli X, Shi N (2015). "Structural basis for the interaction between the Golgi reassembly-stacking protein GRASP65 and the Golgi matrix protein GM130". The Journal of Biological Chemistry. 290 (44): 26373–82. doi:10.1074/jbc.M115.657940. PMC 4646294Freely accessible. PMID 26363069. 
  6. ^ Lane JD, Lucocq J, Pryde J, Barr FA, Woodman PG, Allan VJ, Lowe M (2002). "Caspase-mediated cleavage of the stacking protein GRASP65 is required for Golgi fragmentation during apoptosis". The Journal of Cell Biology. 156 (3): 495–509. doi:10.1083/jcb.200110007. PMC 2173349Freely accessible. PMID 11815631. 
  7. ^ Cheng JP, Betin VM, Weir H, Shelmani GM, Moss DK, Lane JD (2010). "Caspase cleavage of the Golgi stacking factor GRASP65 is required for Fas/CD95-mediated apoptosis". Cell Death & Disease. 1: e82. doi:10.1038/cddis.2010.59. PMC 3035901Freely accessible. PMID 21368855. 
  8. ^ Rabouille C, Linstedt AD (2016). "GRASP: A Multitasking Tether". Frontiers in Cell and Developmental Biology. 4: 1. doi:10.3389/fcell.2016.00001. PMC 4726779Freely accessible. PMID 26858948. 
  9. ^ a b c Barr FA, Preisinger C, Kopajtich R, Körner R (December 2001). "Golgi matrix proteins interact with p24 cargo receptors and aid their efficient retention in the Golgi apparatus". The Journal of Cell Biology. 155 (6): 885–91. doi:10.1083/jcb.200108102. PMC 2150891Freely accessible. PMID 11739402. 
  10. ^ Short B, Preisinger C, Körner R, Kopajtich R, Byron O, Barr FA (December 2001). "A GRASP55-rab2 effector complex linking Golgi structure to membrane traffic". The Journal of Cell Biology. 155 (6): 877–83. doi:10.1083/jcb.200108079. PMC 2150909Freely accessible. PMID 11739401. 
  11. ^ Shorter J, Watson R, Giannakou ME, Clarke M, Warren G, Barr FA (September 1999). "GRASP55, a second mammalian GRASP protein involved in the stacking of Golgi cisternae in a cell-free system". The EMBO Journal. 18 (18): 4949–60. doi:10.1093/emboj/18.18.4949. PMC 1171566Freely accessible. PMID 10487747. 

Further reading[edit]

External Links[edit]

Molecular models of GRASP65/GM130/P115-mediated cis-cisternae membrane stacking and vesicle tethering.