Galactofuranosylgalactofuranosylrhamnosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,5/1,6-galactofuranosyltransferase

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Galactofuranosylgalactofuranosylrhamnosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,5/1,6-galactofuranosyltransferase
Identifiers
EC number2.4.1.288
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum

Galactofuranosylgalactofuranosylrhamnosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,5/1,6-galactofuranosyltransferase (EC 2.4.1.288, GlfT2) is an enzyme with systematic name UDP-alpha-D-galactofuranose:beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol 4-beta/5-beta-D-galactofuranosyltransferase.[1][2][3] This enzyme catalyses the following chemical reaction

28 UDP-alpha-D-galactofuranose + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol 28 UDP + [beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)]14-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol

This enzyme is solated from Mycobacterium tuberculosis.

References[edit]

  1. ^ Rose NL, Zheng RB, Pearcey J, Zhou R, Completo GC, Lowary TL (August 2008). "Development of a coupled spectrophotometric assay for GlfT2, a bifunctional mycobacterial galactofuranosyltransferase". Carbohydrate Research. 343 (12): 2130–9. doi:10.1016/j.carres.2008.03.023. PMID 18423586.
  2. ^ May JF, Splain RA, Brotschi C, Kiessling LL (July 2009). "A tethering mechanism for length control in a processive carbohydrate polymerization". Proceedings of the National Academy of Sciences of the United States of America. 106 (29): 11851–6. Bibcode:2009PNAS..10611851M. doi:10.1073/pnas.0901407106. PMC 2715488. PMID 19571009.
  3. ^ Wheatley RW, Zheng RB, Richards MR, Lowary TL, Ng KK (August 2012). "Tetrameric structure of the GlfT2 galactofuranosyltransferase reveals a scaffold for the assembly of mycobacterial Arabinogalactan". The Journal of Biological Chemistry. 287 (33): 28132–43. doi:10.1074/jbc.M112.347484. PMC 3431634. PMID 22707726.

External links[edit]