3D model (JSmol)
|Molar mass||1198.43 g/mol|
|Safety data sheet||10,000X in water, Biotium Inc.|
|R-phrases (outdated)||R25 R36/37/38|
|S-phrases (outdated)||S22 S24/25 S26 S36/37/39 S45 S53|
|Flash point||> 100 °C (212 °F; 373 K)|
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
GelGreen is an intercalating nucleic acid stain used in molecular biology for agarose gel electrophoresis. GelGreen is structurally closely related to acridinium orange and consists of two acridinium orange subunits that are bridged by a linear spacer.
Its fluorophore, and therefore its optical properties, are essentially identical to those of other N-alkylacridinium orange dyes. When exposed to ultraviolet light, it will fluoresce with a greenish color that strongly intensifies after binding to DNA. The substance is marketed as a less toxic and more sensitive alternative to ethidium bromide. GelGreen is sold as a solution in DMSO or, more recently, in water.
- Ethidium bromide
- SYBR Green I
- Agarose gel electrophoresis and gel electrophoresis of nucleic acids
- Acridine orange
- US application 2010323453, Mao, Fei & Leung, Wai-Yee, "Methods of Using Dyes in Association with Nucleic Acid Staining or Detection and Associated Technology"
- GelRed & GelGreen (PDF), Biotium Inc., August 21, 2012, retrieved December 4, 2012
- GelRed and GelGreen: Environmentally safe and ultra-sensitive nucleic acid gel stains for replacing EtBr, Biotium Inc., retrieved December 4, 2012