Genome size is the total amount of DNA contained within one copy of a single complete genome. It is typically measured in terms of mass in picograms (trillionths (10−12) of a gram, abbreviated pg) or less frequently in daltons, or as the total number of nucleotide base pairs, usually in megabases (millions of base pairs, abbreviated Mb or Mbp). One picogram is equal to 978 megabases. In diploid organisms, genome size is often used interchangeably with the term C-value.
An organism's complexity is not directly proportional to its genome size; total DNA content is widely variable between biological taxa. Some single-celled organisms have much more DNA than humans, for reasons that remain unclear (see non-coding DNA and C-value enigma).
Origin of the term
The term "genome size" is often erroneously attributed to a 1976 paper by Ralph Hinegardner, even in discussions dealing specifically with terminology in this area of research (e.g., Greilhuber 2005). Notably, Hinegardner used the term only once: in the title. The term actually seems to have first appeared in 1968, when Hinegardner wondered, in the last paragraph of another article, whether "cellular DNA content does, in fact, reflect genome size". In this context, "genome size" was being used in the sense of genotype to mean the number of genes.
In a paper submitted only two months later, Wolf et al. (1969) used the term "genome size" throughout and in its present usage; therefore these authors should probably be credited with originating the term in its modern sense. By the early 1970s, "genome size" was in common usage with its present definition, probably as a result of its inclusion in Susumu Ohno's influential book Evolution by Gene Duplication, published in 1970.
Variation in genome size and gene content
With the emergence of various molecular techniques in the past 50 years, the genome sizes of thousands of eukaryotes have been analyzed, and these data are available in online databases for animals, plants, and fungi (see external links). Nuclear genome size is typically measured in eukaryotes using either densitometric measurements of Feulgen-stained nuclei (previously using specialized densitometers, now more commonly using computerized image analysis) or flow cytometry. In prokaryotes, pulsed field gel electrophoresis and complete genome sequencing are the predominant methods of genome size determination.
Nuclear genome sizes are well known to vary enormously among eukaryotic species. In animals they range more than 3,300-fold, and in land plants they differ by a factor of about 1,000. Protist genomes have been reported to vary more than 300,000-fold in size, but the high end of this range (Amoeba) has been called into question.[by whom?] In eukaryotes (but not prokaryotes), genome size is not proportional to the number of genes present in the genome, an observation that was deemed wholly counter-intuitive before the discovery of non-coding DNA and which became known as the "C-value paradox" as a result. However, although there is no longer any paradoxical aspect to the discrepancy between genome size and gene number, the term remains in common usage. For reasons of conceptual clarification, the various puzzles that remain with regard to genome size variation instead have been suggested by one author to more accurately comprise a puzzle or an enigma (the so-called "C-value enigma").
Genome size correlates with a range of measurable characteristics at the cell and organism levels, including cell size, cell division rate, and, depending on the taxon, body size, metabolic rate, developmental rate, organ complexity, geographical distribution, or extinction risk. Based on currently available completely sequenced genome data (as of April 2009), log-transformed gene number forms a linear correlation with log-transformed genome size in bacteria, archaea, viruses, and organelles combined, whereas a nonlinear (semi-natural logarithm) correlation is seen for eukaryotes. Although the latter contrasts with the previous view that no correlation exists for the eukaryotes, the observed nonlinear correlation for eukaryotes may reflect disproportionately fast-increasing non-coding DNA in increasingly large eukaryotic genomes. Although sequenced genome data are practically biased toward small genomes, which may compromise the accuracy of the empirically derived correlation, and ultimate proof of the correlation remains to be obtained by sequencing some of the largest eukaryotic genomes, current data do not seem to rule out a possible correlation.
Human genome size
In humans, the total female diploid nuclear genome per cell extends for 6.37 Gigabase pairs (Gbp), is 208.23 cm long and weighs 6.51 picograms (pg). Male values are 6.27 Gbp, 205.00 cm, 6.41 pg. Each DNA polymer can contain hundreds of millions of nucleotides, such as in chromosome 1. Chromosome 1 is the largest human chromosome with approximately 220 million base pairs, and would be 85 mm long if straightened.
In eukaryotes, in addition to nuclear DNA, there is also mitochondrial DNA (mtDNA) which encodes certain proteins used by the mitochondria. The mtDNA is usually relatively small in comparison to the nuclear DNA. For example, the human mitochondrial DNA forms closed circular molecules, each of which contains 16,569 DNA base pairs, with each such molecule normally containing a full set of the mitochondrial genes. Each human mitochondrion contains, on average, approximately 5 such mtDNA molecules. Each human cell contains approximately 100 mitochondria, giving a total number of mtDNA molecules per human cell of approximately 500. However, the amount of mitochondria per cell also varies by cell type, and an egg cell can contain 100,000 mitochondria, corresponding to up to 1,500,000 copies of the mitochondrial genome (constituting up to 90% of the DNA of the cell).
Genome reduction, also known as genome degradation, is the process by which an organism's genome shrinks relative to that of its ancestors. Genomes fluctuate in size regularly, and genome size reduction is most significant in bacteria.
The most evolutionarily significant cases of genome reduction may be observed in the eukaryotic organelles known to be derived from bacteria: mitochondria and plastids. These organelles are descended from primordial endosymbionts, which were capable of surviving within the host cell and which the host cell likewise needed for survival. Many present-day mitochondria have less than 20 genes in their entire genome, whereas a modern free-living bacterium generally has at least 1,000 genes. Many genes have apparently been transferred to the host nucleus, while others have simply been lost and their function replaced by host processes.
Other bacteria have become endosymbionts or obligate intracellular pathogens and experienced extensive genome reduction as a result. This process seems to be dominated by genetic drift resulting from small population size, low recombination rates, and high mutation rates, as opposed to selection for smaller genomes. Some free-living marine bacterioplanktons also shows signs of genome reduction, which are hypothesized to be driven by natural selection.
In obligate endosymbiotic species
Obligate endosymbiotic species are characterized by a complete inability to survive external to their host environment. These species have become a considerable threat to human health, as they are often capable of evading human immune systems and manipulating the host environment to acquire nutrients. A common explanation for these manipulative abilities is their consistently compact and efficient genomic structure. These small genomes are the result of massive losses of extraneous DNA, an occurrence that is exclusively associated with the loss of a free-living stage. As much as 90% of the genetic material can be lost when a species makes the evolutionary transition from a free-living to an obligate intracellular lifestyle. During this process the future parasite subjected to an environment rich of metabolite where somehow needs to hide within the host cell, those factors reduce the retention and increase the genetic drift leading to an acceleration of the loss of non-essential genes. Common examples of species with reduced genomes include Buchnera aphidicola, Rickettsia prowazekii, and Mycobacterium leprae. One obligate endosymbiont of leafhoppers, Nasuia deltocephalinicola, has the smallest genome currently known among cellular organisms at 112 kb. Despite the pathogenicity of most endosymbionts, some obligate intracellular species have positive fitness effects on their hosts.
The reductive evolution model has been proposed as an effort to define the genomic commonalities seen in all obligate endosymbionts. This model illustrates four general features of reduced genomes and obligate intracellular species:
- "genome streamlining" resulting from relaxed selection on genes that are superfluous in the intracellular environment;
- a bias towards deletions (rather than insertions), which heavily affects genes that have been disrupted by accumulation of mutations (pseudogenes);
- very little or no capability for acquiring new DNA; and
- considerable reduction of effective population size in endosymbiotic populations, particularly in species that rely on vertical transmission of genetic material.
Based on this model, it is clear that endosymbionts face different adaptive challenges than free-living species and, as emerged from the analysis between different parasites, their genes inventories are extremely different, leading us to the conclusion that the genome miniaturization follows a different pattern for the different symbionts.
Conversion from picograms (pg) to base pairs (bp)
In 1991, John W. Drake proposed a general rule: that the mutation rate within a genome and its size are inversely correlated. This rule has been found to be approximately correct for simple genomes such as those in DNA viruses and unicellular organisms. Its basis is unknown.
It has been proposed that the small size of RNA viruses is locked into a three-part relation between replication fidelity, genome size, and genetic complexity. The majority of RNA viruses lack an RNA proofreading facility, which limits their replication fidelity and hence their genome size. This has also been described as the "Eigen paradox". An exception to the rule of small genome sizes in RNA viruses is found in the Nidoviruses. These viruses appear to have acquired a 3′-to-5′ exoribonuclease (ExoN) which has allowed for an increase in genome size.
Genome miniaturization and optimal size
In 1972 Michael David Bennett hypothesized that there was a correlation with the DNA content and the nuclear volume while Commoner and van’t Hof and Sparrow before him postulated that even cell size and cell-cycle length were controlled by the amount of DNA. More recent theories have brought us to discuss about the possibility of the presence of a mechanism that constrains physically the development of the genome to an optimal size.
Those explanations have been disputed by Cavalier-Smith’s article where the author pointed that the way to understand the relation between genome size and cell volume was related to the skeletal theory. The nucleus of this theory is related to the cell volume, determined by an adaptation balance between advantages and disadvantages of bigger cell size, the optimization of the ratio nucleus:cytoplasm (karyoplasmatic ratio) and the concept that larger genomes provides are more prone to the accumulation of duplicative transposons as consequences of higher content of non-coding skeletal DNA. Cavalier-Smith also proposed that, as consequent reaction of a cell reduction, the nucleus will be more prone to a selection in favor for the deletion compared to the duplication.
From the economic way of thinking, since phosphorus and energy are scarce, a reduction in the DNA should be always the focus of the evolution, unless a benefit is acquired. The random deletion will be then mainly deleterious and not selected due to the reduction of the gained fitness but occasionally the elimination will be advantageous as well. This trade-off between economy and accumulation of non-coding DNA is the key to the maintenance of the karyoplasmatic ratio.
Mechanisms of genome miniaturization
The base question behind the process of genome miniaturization is whether it occurs through large steps or due to a constant erosion of the gene content. In order to assess the evolution of this process is necessary to compare an ancestral genome with the one where the shrinkage is supposed to be occurred. Thanks to the similarity among the gene content of Buchnera aphidicola and the enteric bacteria Escherichia coli, 89% identity for the 16S rDNA and 62% for orthologous genes was possible to shed light on the mechanism of genome miniaturization. The genome of the endosymbiont B. aphidicola is characterized by a genome size that is seven times smaller than E. coli (643 kb compared to 4.6 Mb) and can be view as a subset of the enteric bacteria gene inventory. From the confrontation of the two genomes emerged that some genes persist as partially degraded. indicating that the function was lost during the process and that consequent events of erosion shortened the length as documented in Rickettsia. This hypothesis is confirmed by the analysis of the pseudogenes of Buchnera where the number of deletions was more than ten times higher compared to the insertion.
In Rickettsia prowazekii, as with other small genome bacteria, this mutualistic endosymbiont has experienced a vast reduction of functional activity with a major exception compared to other parasites still retain the bio-synthetic ability of production of amino acid needed by its host. The common effects of the genome shrinking between this endosymbiont and the other parasites are the reduction of the ability to produce phospholipids, repair and recombination and an overall conversion of the composition of the gene to a richer A-T content due to mutation and substitutions. Evidence of the deletion of the function of repair and recombination is the loss of the gene recA, gene involved in the recombinase pathway. This event happened during the removal of a larger region containing ten genes for a total of almost 10 kb. Same faith occurred uvrA, uvrB and uvrC, genes encoding for excision enzymes involved in the repair damaged DNA due to UV exposure.
One of the most plausible mechanisms for the explanation of the genome shrinking is the chromosomal rearrangement because insertion/deletion of larger portion of sequence are more easily to be seen in during homologous recombination compared to the illegitimate, therefore the spread of the transposable elements will positively affect the rate of deletion. The loss of those genes in the early stages of miniaturization not only this function but must played a role in the evolution of the consequent deletions. Evidences of the fact that larger event of removal occurred before smaller deletion emerged from the comparison of the genome of Bucknera and a reconstructed ancestor, where the gene that have been lost are in fact not randomly dispersed in the ancestor gene but aggregated and the negative relation between number of lost genes and length of the spacers. The event of small local indels plays a marginal role on the genome reduction especially in the early stages where a larger number of genes became superfluous.
Single events instead occurred due to the lack of selection pressure for the retention of genes especially if part of a pathway that lost its function during a previous deletion. An example for this is the deletion of recF, gene required for the function of recA, and its flanking genes. One of the consequences of the elimination of such amount of sequences affected even the regulation of the remaining genes. The loss of large section of genomes could in fact lead to a loss in promotor sequences. This could in fact pushed the selection for the evolution of polycistronic regions with a positive effect for both size reduction and transcription efficiency.
Evidence of genome miniaturization
One example of the miniaturization of the genome occurred in the microsporidia, an anaerobic intracellular parasite of arthropods evolved from aerobic fungi.
During this process the mitosomes was formed consequent to the reduction of the mitochondria to a relic voided of genomes and metabolic activity except to the production of iron sulfur centers and the capacity to enter into the host cells. Except for the ribosomes, miniaturized as well, many other organelles have been almost lost during the process of the formation of the smallest genome found in the eukaryotes. From their possible ancestor, a zygomycotine fungi, the microsporidia shrunk its genome eliminating almost 1000 genes and reduced even the size of protein and protein-coding genes. This extreme process was possible thanks to the advantageous selection for a smaller cell size imposed by the parasitism.
Another example of miniaturization is represented by the presence of nucleomorphs, enslaved nuclei, inside of the cell of two different algae, cryptophytes and chlorarachneans.
Nucleomorphs are characterized by one of the smallest genomes known (551 and 380 kb) and as noticed for microsporidia, some genomes are noticeable reduced in length compared to other eukaryotes due to a virtual lack of non-coding DNA. The most interesting factor is represented by the coexistence of those small nuclei inside of a cell that contains another nucleus that never experienced such genome reduction. Moreover, even if the host cells have different volumes from species to species and a consequent variability in genome size, the nucleomorph remain invariant denoting a double effect of selection within the same cell.
- ^ a b Dolezel J, Bartoš J, Voglmayr H, Greilhuber J (2003). "Nuclear DNA content and genome size of trout and human". Cytometry Part A. 51 (2): 127–128. doi:10.1002/cyto.a.10013. PMID 12541287.
- ^ a b Hinegardner R (1976). "Evolution of genome size". In F.J. Ayala (ed.). Molecular Evolution. Sinauer Associates, Inc., Sunderland. pp. 179–199.
- ^ Greilhuber J, Doležel J, Lysák M, Bennett MD (2005). "The origin, evolution and proposed stabilization of the terms 'genome size' and 'C-value' to describe nuclear DNA contents". Annals of Botany. 95 (1): 255–260. doi:10.1093/aob/mci019. PMC 4246724. PMID 15596473.
- ^ Hinegardner R (1968). "Evolution of cellular DNA content in teleost fishes". American Naturalist. 102 (928): 517–523. doi:10.1086/282564. S2CID 84409620.
- ^ Wolf U, Ritter H, Atkin NB, Ohno S (1969). "Polyploidization in the fish family Cyprinidae, Order Cypriniformes. I. DNA-content and chromosome sets in various species of Cyprinidae". Humangenetik. 7 (3): 240–244. doi:10.1007/BF00273173. PMID 5800705. S2CID 42045008.
- ^ Ohno S (1970). Evolution by Gene Duplication. New York: Springer-Verlag. ISBN 0-04-575015-7.
- ^ Hardie DC, Gregory TR, Hebert PD (2002). "From pixels to picograms: a beginners' guide to genome quantification by Feulgen image analysis densitometry". Journal of Histochemistry and Cytochemistry. 50 (6): 735–749. doi:10.1177/002215540205000601. PMID 12019291.
- ^ a b Bennett MD, Leitch IJ (2005). "Genome size evolution in plants". In T.R. Gregory (ed.). The Evolution of the Genome. San Diego: Elsevier. pp. 89–162.
- ^ a b Gregory TR (2005). "Genome size evolution in animals". In T.R. Gregory (ed.). The Evolution of the Genome. San Diego: Elsevier. pp. 3–87.
- ^ Hou Y, Lin S (2009). Redfield RJ (ed.). "Distinct Gene Number- Genome Size Relationships for Eukaryotes and Non-Eukaryotes: Gene Content Estimation for Dinoflagellate Genomes". PLOS ONE. 4 (9): e6978. Bibcode:2009PLoSO...4.6978H. doi:10.1371/journal.pone.0006978. PMC 2737104. PMID 19750009.
- ^ a b Piovesan A, Pelleri MC, Antonaros F, Strippoli P, Caracausi M, Vitale L (2019). "On the length, weight and GC content of the human genome". BMC Res Notes. 12 (1): 106. doi:10.1186/s13104-019-4137-z. PMC 6391780. PMID 30813969.
- ^ Gregory SG, Barlow KF, McLay KE, Kaul R, Swarbreck D, Dunham A, et al. (May 2006). "The DNA sequence and biological annotation of human chromosome 1". Nature. 441 (7091): 315–21. Bibcode:2006Natur.441..315G. doi:10.1038/nature04727. PMID 16710414.
- ^ Anderson, S.; Bankier, A. T.; Barrell, B. G.; de Bruijn, M. H. L.; Coulson, A. R.; Drouin, J.; Eperon, I. C.; Nierlich, D. P.; Roe, B. A.; Sanger, F.; Schreier, P. H.; Smith, A. J. H.; Staden, R.; Young, I. G. (April 1981). "Sequence and organization of the human mitochondrial genome". Nature. 290 (5806): 457–465. Bibcode:1981Natur.290..457A. doi:10.1038/290457a0. PMID 7219534. S2CID 4355527.
- ^ "Untitled". Archived from the original on 2011-08-13. Retrieved 2012-06-13.
- ^ a b c Satoh, M; Kuroiwa, T (September 1991). "Organization of multiple nucleoids and DNA molecules in mitochondria of a human cell". Experimental Cell Research. 196 (1): 137–140. doi:10.1016/0014-4827(91)90467-9. PMID 1715276.
- ^ Zhang D, Keilty D, Zhang ZF, Chian RC (2017). "Mitochondria in oocyte aging: current understanding". Facts Views Vis Obgyn. 9 (1): 29–38. PMC 5506767. PMID 28721182.
- ^ Dufresne A, Garczarek L, Partensky F (2005). "Accelerated evolution associated with genome reduction in a free-living prokaryote". Genome Biol. 6 (2): R14. doi:10.1186/gb-2005-6-2-r14. PMC 551534. PMID 15693943.
- ^ Giovannoni SJ; et al. (2005). "Genome streamlining in a cosmopolitan oceanic bacterium". Science. 309 (5738): 1242–1245. Bibcode:2005Sci...309.1242G. doi:10.1126/science.1114057. PMID 16109880. S2CID 16221415.
- ^ Giovannoni SJ; et al. (2008). "The small genome of an abundant coastal ocean methylotroph". Environmental Microbiology. 10 (7): 1771–1782. doi:10.1111/j.1462-2920.2008.01598.x. PMID 18393994.
- ^ a b Moran, N. A. (1996-04-02). "Accelerated evolution and Muller's rachet in endosymbiotic bacteria". Proceedings of the National Academy of Sciences. 93 (7): 2873–2878. Bibcode:1996PNAS...93.2873M. doi:10.1073/pnas.93.7.2873. ISSN 0027-8424. PMC 39726. PMID 8610134.
- ^ Wernegreen, J. J.; Moran, N. A. (1999-01-01). "Evidence for genetic drift in endosymbionts (Buchnera): analyses of protein-coding genes". Molecular Biology and Evolution. 16 (1): 83–97. doi:10.1093/oxfordjournals.molbev.a026040. ISSN 0737-4038. PMID 10331254.
- ^ Spaulding, Allen W.; Dohlen, Carol D. von (2001). "Psyllid endosymbionts exhibit patterns of co-speciation with hosts and destabilizing substitutions in ribosomal RNA". Insect Molecular Biology. 10 (1): 57–67. doi:10.1046/j.1365-2583.2001.00231.x. ISSN 1365-2583. PMID 11240637. S2CID 46186732.
- ^ And the Genomes Keep Shrinking…
- ^ Wernegreen J (2005). "For better or worse: Genomic consequences of genomic mutualism and parasitism" (PDF). Current Opinion in Genetics & Development. 15 (6): 1–12. doi:10.1016/j.gde.2005.09.013. PMID 16230003. Archived from the original (PDF) on 2011-07-22.
- ^ Moran NA, Plague GR (2004). "Genomic changes following host restriction in bacteria". Current Opinion in Genetics & Development. 14 (6): 627–633. doi:10.1016/j.gde.2004.09.003. PMID 15531157.
- ^ Mushegian, A. R.; Koonin, E. V. (1996-09-17). "A minimal gene set for cellular life derived by comparison of complete bacterial genomes". Proceedings of the National Academy of Sciences. 93 (19): 10268–10273. Bibcode:1996PNAS...9310268M. doi:10.1073/pnas.93.19.10268. ISSN 0027-8424. PMC 38373. PMID 8816789.
- ^ Huynen, Martijn A.; Bork, Peer (1998-05-26). "Measuring genome evolution". Proceedings of the National Academy of Sciences. 95 (11): 5849–5856. Bibcode:1998PNAS...95.5849H. doi:10.1073/pnas.95.11.5849. ISSN 0027-8424. PMC 34486. PMID 9600883.
- ^ Maniloff, J (1996-09-17). "The minimal cell genome: "on being the right size"". Proceedings of the National Academy of Sciences of the United States of America. 93 (19): 10004–10006. Bibcode:1996PNAS...9310004M. doi:10.1073/pnas.93.19.10004. ISSN 0027-8424. PMC 38325. PMID 8816738.
- ^ Drake, J W (1991). "A constant rate of spontaneous mutation in DNA-based microbes". Proc Natl Acad Sci USA. 88 (16): 7160–7164. Bibcode:1991PNAS...88.7160D. doi:10.1073/pnas.88.16.7160. PMC 52253. PMID 1831267.
- ^ Kun, A; Santos, M; Szathmary, E (2005). "Real ribozymes suggest a relaxed error threshold". Nat Genet. 37 (9): 1008–1011. doi:10.1038/ng1621. PMID 16127452. S2CID 30582475.
- ^ Lauber, C; Goeman, JJ; Parquet Mdel, C; Thi Nga, P; Snijder, EJ; Morita, K; Gorbalenya, AE (Jul 2013). "The footprint of genome architecture in the largest genome expansion in RNA viruses". PLOS Pathog. 9 (7): e1003500. doi:10.1371/journal.ppat.1003500. PMC 3715407. PMID 23874204.
- ^ Bennett, Michael David; Riley, Ralph (1972-06-06). "Nuclear DNA content and minimum generation time in herbaceous plants". Proceedings of the Royal Society of London. Series B. Biological Sciences. 181 (1063): 109–135. Bibcode:1972RSPSB.181..109B. doi:10.1098/rspb.1972.0042. PMID 4403285. S2CID 26642634.
- ^ Hof, J. Van't; Sparrow, A. H. (June 1963). "A relationship between DNA content, nuclear volume, and minimum mitotic cycle time". Proceedings of the National Academy of Sciences of the United States of America. 49 (6): 897–902. Bibcode:1963PNAS...49..897V. doi:10.1073/pnas.49.6.897. ISSN 0027-8424. PMC 300029. PMID 13996145.
- ^ Commoner, Barry (June 1964). "Roles Of Deoxyribonucleic Acid in Inheritance". Nature. 202 (4936): 960–968. Bibcode:1964Natur.202..960C. doi:10.1038/202960a0. ISSN 1476-4687. PMID 14197326. S2CID 4166234.
- ^ Orgel, L. E.; Crick, F. H. C. (April 1980). "Selfish DNA: the ultimate parasite". Nature. 284 (5757): 604–607. Bibcode:1980Natur.284..604O. doi:10.1038/284604a0. ISSN 1476-4687. PMID 7366731. S2CID 4233826.
- ^ a b c d e f Cavalier-Smith, Thomas (2005-01-01). "Economy, Speed and Size Matter: Evolutionary Forces Driving Nuclear Genome Miniaturization and Expansion". Annals of Botany. 95 (1): 147–175. doi:10.1093/aob/mci010. ISSN 0305-7364. PMC 4246715. PMID 15596464.
- ^ Strasburger, Eduard (1893). Ueber die wirkungssphäre der Kerne und die Zellgrösse (in German). OCLC 80359142.
- ^ Huxley, J. S. (May 1925). "The Cell in Development and Heredity". Nature. 115 (2897): 669–671. Bibcode:1925Natur.115..669H. doi:10.1038/115669a0. ISSN 1476-4687. S2CID 26264738.
- ^ a b c d Moran, Nancy A.; Mira, Alex (2001-11-14). "The process of genome shrinkage in the obligate symbiont Buchnera aphidicola". Genome Biology. 2 (12): research0054.1. doi:10.1186/gb-2001-2-12-research0054. ISSN 1474-760X. PMC 64839. PMID 11790257.
- ^ Blattner, Frederick R.; Plunkett, Guy; Bloch, Craig A.; Perna, Nicole T.; Burland, Valerie; Riley, Monica; Collado-Vides, Julio; Glasner, Jeremy D.; Rode, Christopher K.; Mayhew, George F.; Gregor, Jason (1997-09-05). "The Complete Genome Sequence of Escherichia coli K-12". Science. 277 (5331): 1453–1462. doi:10.1126/science.277.5331.1453. ISSN 0036-8075. PMID 9278503.
- ^ a b c d e Shigenobu, Shuji; Watanabe, Hidemi; Hattori, Masahira; Sakaki, Yoshiyuki; Ishikawa, Hajime (September 2000). "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS". Nature. 407 (6800): 81–86. Bibcode:2000Natur.407...81S. doi:10.1038/35024074. ISSN 1476-4687. PMID 10993077.
- ^ Andersson, J. O.; Andersson, S. G. (1999-09-01). "Genome degradation is an ongoing process in Rickettsia". Molecular Biology and Evolution. 16 (9): 1178–1191. doi:10.1093/oxfordjournals.molbev.a026208. ISSN 0737-4038. PMID 10486973.
- ^ Andersson, Jan O.; Andersson, Siv G. E. (2001-05-01). "Pseudogenes, Junk DNA, and the Dynamics of Rickettsia Genomes". Molecular Biology and Evolution. 18 (5): 829–839. doi:10.1093/oxfordjournals.molbev.a003864. ISSN 0737-4038. PMID 11319266.
- ^ a b Mira, Alex; Ochman, Howard; Moran, Nancy A. (2001-10-01). "Deletional bias and the evolution of bacterial genomes". Trends in Genetics. 17 (10): 589–596. doi:10.1016/S0168-9525(01)02447-7. ISSN 0168-9525. PMID 11585665.
- ^ a b c Andersson, Siv G. E.; Zomorodipour, Alireza; Andersson, Jan O.; Sicheritz-Pontén, Thomas; Alsmark, U. Cecilia M.; Podowski, Raf M.; Näslund, A. Kristina; Eriksson, Ann-Sofie; Winkler, Herbert H.; Kurland, Charles G. (November 1998). "The genome sequence of Rickettsia prowazekii and the origin of mitochondria". Nature. 396 (6707): 133–140. Bibcode:1998Natur.396..133A. doi:10.1038/24094. ISSN 1476-4687. PMID 9823893.
- ^ Tamas, Ivica; Klasson, Lisa M.; Sandström, Jonas P.; Andersson, Siv G. E. (2001). "Mutualists and parasites: how to paint yourself into a (metabolic) corner". FEBS Letters. 498 (2–3): 135–139. doi:10.1016/S0014-5793(01)02459-0. ISSN 1873-3468. PMID 11412844. S2CID 40955247.
- ^ Wernegreen, J. J.; Moran, N. A. (2000-07-22). "Decay of mutualistic potential in aphid endosymbionts through silencing of biosynthetic loci: Buchnera of Diuraphis". Proceedings of the Royal Society of London. Series B: Biological Sciences. 267 (1451): 1423–1431. doi:10.1098/rspb.2000.1159. PMC 1690690. PMID 10983826.
- ^ Petrov, Dmitri A. (2002-06-01). "Mutational Equilibrium Model of Genome Size Evolution". Theoretical Population Biology. 61 (4): 531–544. doi:10.1006/tpbi.2002.1605. ISSN 0040-5809. PMID 12167373.
- ^ Gregory, T. Ryan (2003-09-01). "Is small indel bias a determinant of genome size?". Trends in Genetics. 19 (9): 485–488. doi:10.1016/S0168-9525(03)00192-6. ISSN 0168-9525. PMID 12957541.
- ^ Gasior, Stephen L.; Olivares, Heidi; Ear, Uy; Hari, Danielle M.; Weichselbaum, Ralph; Bishop, Douglas K. (2001-07-17). "Assembly of RecA-like recombinases: Distinct roles for mediator proteins in mitosis and meiosis". Proceedings of the National Academy of Sciences. 98 (15): 8411–8418. Bibcode:2001PNAS...98.8411G. doi:10.1073/pnas.121046198. ISSN 0027-8424. PMC 37451. PMID 11459983.
- ^ Selosse, M.-A.; Albert, B.; Godelle, B. (2001-03-01). "Reducing the genome size of organelles favours gene transfer to the nucleus". Trends in Ecology & Evolution. 16 (3): 135–141. doi:10.1016/s0169-5347(00)02084-x. ISSN 1872-8383. PMID 11179577.
- ^ Scherbakov, D. V.; Garber, M. B. (2000-07-01). "Overlapping genes in bacterial and phage genomes". Molecular Biology. 34 (4): 485–495. doi:10.1007/BF02759558. ISSN 1608-3245. S2CID 24144602.
- ^ Williams, Bryony A. P.; Hirt, Robert P.; Lucocq, John M.; Embley, T. Martin (August 2002). "A mitochondrial remnant in the microsporidian Trachipleistophora hominis". Nature. 418 (6900): 865–869. Bibcode:2002Natur.418..865W. doi:10.1038/nature00949. ISSN 1476-4687. PMID 12192407. S2CID 4358253.
- ^ Keeling, Patrick J.; Fast, Naomi M. (2002). "Microsporidia: Biology and Evolution of Highly Reduced Intracellular Parasites". Annual Review of Microbiology. 56 (1): 93–116. doi:10.1146/annurev.micro.56.012302.160854. PMID 12142484. S2CID 22943269.
- ^ Cavalier-Smith, T. (2001). "What are Fungi?". In McLaughlin, David J.; McLaughlin, Esther G.; Lemke, Paul A. (eds.). Systematics and Evolution. Systematics and Evolution: Part A. The Mycota. Springer Berlin Heidelberg. pp. 3–37. doi:10.1007/978-3-662-10376-0_1. ISBN 978-3-662-10376-0.
- ^ Vivarès, Christian P; Gouy, Manolo; Thomarat, Fabienne; Méténier, Guy (2002-10-01). "Functional and evolutionary analysis of a eukaryotic parasitic genome". Current Opinion in Microbiology. 5 (5): 499–505. doi:10.1016/S1369-5274(02)00356-9. ISSN 1369-5274. PMID 12354558.
- ^ Douglas, Susan; Zauner, Stefan; Fraunholz, Martin; Beaton, Margaret; Penny, Susanne; Deng, Lang-Tuo; Wu, Xiaonan; Reith, Michael; Cavalier-Smith, Thomas; Maier, Uwe-G. (April 2001). "The highly reduced genome of an enslaved algal nucleus". Nature. 410 (6832): 1091–1096. Bibcode:2001Natur.410.1091D. doi:10.1038/35074092. ISSN 1476-4687. PMID 11323671.
- Evolution of Chlamydiaceae
- Andersson JO Andersson SG; Andersson (1999). "Genome degradation is an ongoing process in Rickettsia". Molecular Biology and Evolution. 16 (9): 1178–1191. doi:10.1093/oxfordjournals.molbev.a026208. PMID 10486973. Archived from the original on 2005-04-17. Retrieved 2006-10-18.
- Animal Genome Size Database
- Plant DNA C-values Database
- Fungal Genome Size Database
- Fungal Database Archived 2008-03-10 at the Wayback Machine — by CBS