SR 253 begins in Seminole County, at the entrance to Trail's End Resort and Marina, on the shore of Lake Seminole, which separates Florida and Georgia. It heads northeast and curves to the east, to an intersection with SR 39, at the northwest corner of Seminole State Park. It runs northeast until it intersects SR 374. SR 253 continues heading northeast, past Reynoldsville State Park, into Decatur County, and over a northern branch of Lake Seminole, until it meets SR 310 (Yates Spring Road). The highway continues its northeast trek until it enters Bainbridge.
In Bainbridge, it meets SR 253 Spur (Airport Road), before it crosses under US 27/US 84/SR 1/SR 38 (Wiregrass Georgia Parkway). Farther to the northeast is a short concurrency with US 27 Business/SR 1 Business (Dothan Road). The route then leaves the city to continue to the northeast, passing through rural areas, and crossing into Baker County, before meeting its northern terminus, an intersection with SR 91, southwest of Newton.
SR 253 is not part of the National Highway System, a system of roadways important to the nation's economy, defense, and mobility.