SR 335 begins at an intersection with SR 15 Alt./SR 82 (Sycamore Street) in Jefferson. The highway travels to the east, through rural portions of Jackson County. At Yz Sailor Road, the name changes from Danielsville Street to Brockton Road, and at Rabbit Run it becomes Jefferson Drive until it meets its eastern terminus, an intersection with US 441/SR 15 (Main Street) in Nicholson. Here, the roadway continues as Mulberry Street.
SR 335 is not part of the National Highway System, a system of roadways important to the nation's economy, defense, and mobility.