Glutamyl endopeptidase II

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Glutamyl endopeptidase II
Identifiers
EC number 3.4.21.82
CAS number 137010-42-5
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

Glutamyl endopeptidase II (EC 3.4.21.82, GluSGP) is an enzyme.[1][2][3][4][5] This enzyme catalyses the following chemical reaction

Preferential cleavage: -Glu- >> -Asp- . Preference for Pro or Leu at P2 and Phe at P3. Cleavage of -Glu-Asp- and -Glu-Pro- bonds is slow

This enzyme is isolated from Streptomyces griseus.

References[edit]

  1. ^ Yoshida, N.; Tsuruyama, S.; Nagata, K.; Hirayama, K.; Noda, K.; Makisumi, S. (1988). "Purification and characterization of an acidic amino acid specific endopeptidase of Streptomyces griseus obtained from a commercial preparation (Pronase)". J. Biochem. (Tokyo). 104: 451–456. PMID 3149277. 
  2. ^ Komiyama, T.; Bigler, T.L.; Yoshida, N.; Noda, K.; Laskowski, M. Jr. (1991). "Replacement of P1 Leu18 by Glu18 in the reactive site of turkey ovomucoid third domain converts it into a strong inhibitor of Glu-specific Streptomyces griseus Proteinase (GluSGP)". J. Biol. Chem. 266: 10727–10730. PMID 1674942. 
  3. ^ Nagata, K.; Yoshida, N.; Ogata, F.; Araki, M.; Noda, K. (1991). "Subsite mapping of an acidic amino acid-specific endopeptidase from Streptomyces griseus, GluSGP, and protease V8". J. Biochem. (Tokyo). 110: 859–862. PMID 1794975. 
  4. ^ Svendsen, I.; Jensen, M.R.; Breddam, K. (1991). "The primary structure of the glutamic acid-specific protease of Streptomyces griseus". FEBS Lett. 292: 165–167. PMID 1959600. doi:10.1016/0014-5793(91)80859-2. 
  5. ^ Breddam, K.; Meldal, M. (1992). "Substrate preferences of glutamic-acid-specific endopeptidases assessed by synthetic peptide substrates based on intramolecular fluorescence quenching". Eur. J. Biochem. 206: 103–107. PMID 1587264. doi:10.1111/j.1432-1033.1992.tb16906.x. 

External links[edit]