Glutathione synthase

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glutathione synthase
glutathione synthetase dimer, Human
EC number6.3.2.3
CAS number9023-62-5
IntEnzIntEnz view
ExPASyNiceZyme view
MetaCycmetabolic pathway
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO

In enzymology, a glutathione synthase (EC is an enzyme that catalyzes the chemical reaction

ATP + gamma-L-glutamyl-L-cysteine + glycine ADP + phosphate + glutathione

The 3 substrates of this enzyme are ATP, gamma-L-glutamyl-L-cysteine, and glycine, whereas its 3 products are ADP, phosphate, and glutathione.

This enzyme belongs to the family of ligases, specifically those forming carbon-nitrogen bonds as acid-D-amino-acid ligases (peptide synthases). The systematic name of this enzyme class is gamma-L-glutamyl-L-cysteine:glycine ligase (ADP-forming). Other names in common use include glutathione synthetase, and GSH synthetase. This enzyme participates in glutamate metabolism and glutathione metabolism. At least one compound, Phosphinate is known to inhibit this enzyme.

Structural studies[edit]

As of late 2007, 7 structures have been solved for this class of enzymes, with PDB accession codes 1GLV, 1GSA, 1GSH, 1M0T, 1M0W, 2GLT, and 2HGS.


  • Law MY, Halliwell B (1986). "Purification and properties of glutathione synthetase from (Spinacia oleracea) leaves". Plant Sci. 43 (3): 185–191. doi:10.1016/0168-9452(86)90016-6.
  • Macnicol PK (1987). "Homoglutathione and glutathione synthetases of legume seedlings - partial-purification and substrate-specificity". Plant Sci. 53 (3): 229–235. doi:10.1016/0168-9452(87)90159-2.