The west end of the GR-44 is in downtown Thebes, where it connects with the GR-3. It crosses Motorway 1 east of Thebes, and runs north to Chalcis. At Chalcis it crosses the Euripus Strait, and enters the island of Euboea. The GR-77 towards northern Euboea branches off. The GR-44 continues to the southeastern part of the island, passing along the south coast through the towns Eretria, Amarynthos and Aliveri. Beyond Aliveri it leaves the coast, and it runs south through Styra, ending in Karystos.
The Greek National Road 44 passes through the following places: