|Minor league affiliations|
|Appalachian League (2004–2017)|
|Major league affiliations|
|Minor league titles|
|League titles (3)|
|Division titles (2)|
|Colors||Navy blue, orange, white|
The Greeneville Astros were a Minor League Baseball team of the Rookie League Appalachian League. They were located in Tusculum, Tennessee, and played their home games at Pioneer Park on the campus of Tusculum College. They were an affiliate of the Houston Astros from 2004 to 2017 when the Astros decided to eliminate their Greeneville team and reduce their farm system from nine teams to eight. The Greeneville Astros were replaced by the Cincinnati Reds affiliated Greeneville Reds who assumed the Greeneville Astros spot in the Appalachian League; the Greeneville Reds are not a continuation of the Greeneville Astros and are instead a separate franchise.
In 2007, the first former Greeneville Astros made it to the major leagues. Four players from the 2004 Greenville team were called up to Houston late in the 2007 season: Juan Gutiérrez, Felipe Paulino, Troy Patton, and J. R. Towles.
- José Altuve (2008, 2010) 5x MLB All-Star; 3x AL Batting Title (2014-2015, 2017)
- Carlos Correa (2012) MLB All-Star; 2016 AL Rookie of the Year
- Ivan DeJesus (2006, MGR)
- Mike Lieberthal (1990) 2x MLB All-Star
- Lance McCullers (2012) MLB All-Star
- J.D. Martinez (2009) MLB All-Star
- Russ Nixon (2005, MGR)
- 2004: Defeated Danville Braves 2–1 to win Appalachian League championship.
- 2013: Defeated Kingsport Mets 2–1 in semifinals; lost to Pulaski Mariners 2–0 in finals.
- 2015: Defeated Kingsport Mets 2–1 in semifinals; defeated Princeton Rays 2–1 to win Appalachian League championship.
- 2017: Lost to Elizabethton Twins 2–1 in semifinals.
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