Guanylate kinase

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guanylate kinase
2an9.jpg
guanylate kinase homohexamer, E.Coli
Identifiers
EC no.2.7.4.8
CAS no.9026-59-9
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO
Search
PMCarticles
PubMedarticles
NCBIproteins
guanylate kinase
PDB 1gky EBI.jpg
Structure of Guanylate Kinase.[1]
Identifiers
SymbolGuanylate_kin
PfamPF00625
InterProIPR008144
PROSITEPDOC00670
SCOP21gky / SCOPe / SUPFAM
CDDcd00071
Available protein structures:
Pfam  structures / ECOD  
PDBRCSB PDB; PDBe; PDBj
PDBsumstructure summary
PDB1ex6​, 1ex7​, 1gky​, 1jxm​, 1jxo​, 1kgd​, 1kjw​, 1lvg​, 1s4q​, 1s96​, 1xzp​, 1xzq​, 1z6g​, 1z8f​, 1znw​, 1znx​, 1zny​, 1znz​, 2an9​, 2anb​, 2anc​, 2f3r​, 2f3t​, 2qor

In enzymology, a guanylate kinase (EC 2.7.4.8) is an enzyme that catalyzes the chemical reaction

ATP + GMP ADP + GDP

Thus, the two substrates of this enzyme are ATP and GMP, whereas its two products are ADP and GDP.

This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with a phosphate group as acceptor. This enzyme participates in purine metabolism.

Guanylate kinase catalyzes the ATP-dependent phosphorylation of GMP into GDP.[1] It is essential for recycling GMP and indirectly, cGMP. In prokaryotes (such as Escherichia coli), lower eukaryotes (such as yeast) and in vertebrates, GK is a highly conserved monomeric protein of about 200 amino acids. GK has been shown to be structurally similar to protein A57R (or SalG2R) from various strains of Vaccinia virus.[2][3][4] Systems biology analyses carried out by the team of Andreas Dräger also identified a pivotal role of this enzyme in the replication of SARS-CoV-2 within the human airways.[5][6]

Nomenclature[edit]

The systematic name of this enzyme class is ATP:(d)GMP phosphotransferase. Other names in common use include"

  • deoxyguanylate kinase,
  • 5'-GMP kinase,
  • GMP kinase,
  • guanosine monophosphate kinase, and
  • ATP:GMP phosphotransferase.

References[edit]

  1. ^ a b Stehle T, Schulz GE (April 1992). "Refined structure of the complex between guanylate kinase and its substrate GMP at 2.0 A resolution". J. Mol. Biol. 224 (4): 1127–41. doi:10.1016/0022-2836(92)90474-X. PMID 1314905.
  2. ^ Bryant PJ, Woods DF (February 1992). "A major palmitoylated membrane protein of human erythrocytes shows homology to yeast guanylate kinase and to the product of a Drosophila tumor suppressor gene". Cell. 68 (4): 621–2. doi:10.1016/0092-8674(92)90136-Z. PMID 1310897. S2CID 46607652.
  3. ^ Zschocke PD, Schiltz E, Schulz GE (April 1993). "Purification and sequence determination of guanylate kinase from pig brain". Eur. J. Biochem. 213 (1): 263–9. doi:10.1111/j.1432-1033.1993.tb17757.x. PMID 8097461.
  4. ^ Goebl MG (March 1992). "Is the erythrocyte protein p55 a membrane-bound guanylate kinase?". Trends Biochem. Sci. 17 (3): 99. doi:10.1016/0968-0004(92)90244-4. PMID 1329277.
  5. ^ Renz, Alina; Widerspick, Lina; Dräger, Andreas (2020). "FBA reveals guanylate kinase as a potential target for antiviral therapies against SARS-CoV-2". Bioinformatics. 36 (Supplement_2): i813–i821. doi:10.1093/bioinformatics/btaa813. PMC 7773487. PMID 33381848.
  6. ^ Renz, Alina; Widerspick, Lina; Dräger, Andreas (2021). "Genome-Scale Metabolic Model of Infection with SARS-CoV-2 Mutants Confirms Guanylate Kinase as Robust Potential Antiviral Target". Genes. 12 (6): 796. doi:10.3390/genes12060796. PMC 8225150. PMID 34073716.

Further reading[edit]

This article incorporates text from the public domain Pfam and InterPro: IPR008144