Neonatal heel prick
The neonatal heel prick or Guthrie test is a screening test done on newborns. It consists of making a pinprick puncture in one heel of the newborn and soaking the blood into pre-printed collection cards known as Guthrie cards.
The classical Guthrie test is named after Robert Guthrie, an American bacteriologist and physician who devised it in 1962. The test has been widely used throughout North America and Europe as one of the core newborn screening tests since the late 1960s. The test was initially a bacterial inhibition assay, but is gradually being replaced in many areas by newer techniques such as tandem mass spectrometry that can detect a wider variety of congenital diseases.
The blood samples can be used for a variety of metabolic test to detect genetic conditions, including:
- Immunoreactive Trypsinogen (IRT) to detect cystic fibrosis.
- Maple syrup urine disease (MSUD or Branched Chain Ketonuria) a rare disorder where an error in metabolism inhibits the breakdown of amino acid leucine, isoleucine and valine. It can impair brain development.
- Medium-chain acyl-coenzyme A dehydrogenase deficiency (MCADD)
- Phenylketonuria, a disorder where an error in amino acid metabolism can impair brain development (PKU)
- Sickle-cell disease
- Thyroid stimulating hormone (TSH) to detect congenital hypothyroidism and hence prevent cretinism.
The test uses the growth of a strain of bacteria on a specially-prepared agar plate as a sign for the presence of high levels of phenylalanine, phenylpyruvate, and/or phenyllactate. The compound B-2-thienylalanine will inhibit the growth of the bacterium Bacillus subtilis (ATCC 6051) on minimal culture media. If phenylalanine, phenylpyruvate, and/or phenyllactate is added to the medium, then growth is restored. Such compounds will be present in excess in the blood or urine of patients with PKU. If a suitably-prepared sample of blood or urine is applied to the seeded agar plate, the growth of the bacteria in the test will be a positive indicator for PKU in the patient.
To prepare the sample for application, a small amount of blood (from a heel puncture, for example) or urine (from a diaper, for example) is applied to a piece of filter paper. Then a small disc is punched from the center of the spot of blood or urine, and the disc applied to the surface of a seeded, minimal-medium agar plate that contains added beta-2-thienylalanine. If the sample contains phenylalanine, phenylpyruvate, and/or phenyllactate then these compounds will diffuse into the agar medium. If their concentrations are high enough (as with the excess levels seen with PKU), bacteria will grow under the disc, but not elsewhere. Generally an overnight incubation is enough to determine whether phenylalanine, phenylpyruvate, and/or phenyllactate are present in unusual concentrations in blood or urine.
The blood spot sample should be taken on day 5 and in exceptional circumstances between day 5 and day 8 for all babies regardless of medical condition, milk feeding and prematurity. For the purpose of screening, date of birth is day 0 (some IT systems record date of birth as day 1). False positives and false negatives can sometimes occur when the screening tests are performed before 48 hours.
With genetic tests becoming more common, a wide variety of tests may use the blood drawn by this method. Many neonatal units (SCBUs) now use this method to carry out the daily blood tests (blood count, electrolytes) required to check the progress of ill neonates.
In Ireland, a controversy emerged in 2012 whereby a number of hospitals retained heel prick test cards and thereby a DNA database with over a million samples from 1984, without consent or notification of parents. This resulted in a ten-year rolling destruction cycle being introduced. Similar practices exist in the United Kingdom, New Zealand, and several states of the United States. 
- Guthrie Cards, Catalyst (ABC1), 29 May 2003.
- Julia A. McMillan; Ralph D. Feigin; Catherine DeAngelis; M. Douglas Jones (1 April 2006). Oski's pediatrics: principles & practice. Lippincott Williams & Wilkins. pp. 162–. ISBN 978-0-7817-3894-1. Retrieved 16 April 2010.
- "PHE-TST001". uic.edu.
- "Standards". screening.nhs.uk.
- "Page not found". health.vic.gov.au.
- "International standards for storing samples vary wildly". Irish Times.