Cocaine esterase

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Cocaine esterase
EC number
IntEnz IntEnz view
ExPASy NiceZyme view
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

Cocaine esterase (EC, CocE, hCE2, hCE-2, human carboxylesterase 2) is an enzyme with systematic name cocaine benzoylhydrolase.[1][2][3][4][5] This enzyme catalyses the following chemical reaction

cocaine + H2O ecgonine methyl ester + benzoate

Rhodococcus sp. strain MB1 and Pseudomonas maltophilia strain MB11L can utilize cocaine as sole source of carbon and energy.

This enzyme and redesigned versions of it have been studied as a potential treatment for cocaine addiction in humans.[6]


  1. ^ Gao, D.; Narasimhan, D.L.; Macdonald, J.; Brim, R.; Ko, M.C.; Landry, D.W.; Woods, J.H.; Sunahara, R.K.; Zhan, C.G. (2009). "Thermostable variants of cocaine esterase for long-time protection against cocaine toxicity". Mol. Pharmacol. 75 (2): 318–323. PMC 2684895Freely accessible. PMID 18987161. doi:10.1124/mol.108.049486. 
  2. ^ Bresler, M.M.; Rosser, S.J.; Basran, A.; Bruce, N.C. (2000). "Gene cloning and nucleotide sequencing and properties of a cocaine esterase from Rhodococcus sp. strain MB1". Appl. Environ. Microbiol. 66 (3): 904–908. PMC 91920Freely accessible. PMID 10698749. doi:10.1128/aem.66.3.904-908.2000. 
  3. ^ Britt, A.J.; Bruce, N.C.; Lowe, C.R. (1992). "Identification of a cocaine esterase in a strain of Pseudomonas maltophilia". J. Bacteriol. 174 (7): 2087–2094. PMC 205824Freely accessible. PMID 1551831. 
  4. ^ Larsen, N.A.; Turner, J.M.; Stevens, J.; Rosser, S.J.; Basran, A.; Lerner, R.A.; Bruce, N.C.; Wilson, I.A. (2002). "Crystal structure of a bacterial cocaine esterase". Nat. Struct. Biol. 9 (1): 17–21. PMID 11742345. doi:10.1038/nsb742. 
  5. ^ Pindel, E.V.; Kedishvili, N.Y.; Abraham, T.L.; Brzezinski, M.R.; Zhang, J.; Dean, R.A.; Bosron, W.F. (1997). "Purification and cloning of a broad substrate specificity human liver carboxylesterase that catalyzes the hydrolysis of cocaine and heroin". J. Biol. Chem. 272: 14769–14775. PMID 9169443. doi:10.1074/jbc.272.23.14769. 
  6. ^ Narasimhan, D.; Woods, J.; Sunahara, R. (2012). "Bacterial cocaine esterase: a protein-based therapy for cocaine overdose and addiction". Future Medicinal Chemistry. 4: 137–150. doi:10.4155/fmc.11.194. 

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