The protein encoded by this gene belongs to the heat shock protein 70 family. This gene has three mRNAs from the use of alternative transcription sites. A cis-acting segment is found at the 5' end of exon 1A which is involved in the stress-dependent induction. The transcript that begins with exon 1B is preferentially induced by hypoxia, resulting in the accumulation of this protein in the endoplasmic reticulum (ER). The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. There is also an alternative translation site of this gene which lacks the signal peptide. This signal peptide-lacking protein, is only 3 amino acids shorter than the mature protein in the ER, and it is thought to have a housekeeping function in the cytosol.
^Ikeda J, Kaneda S, Kuwabara K, Ogawa S, Kobayashi T, Matsumoto M, Yura T, Yanagi H (Feb 1997). "Cloning and expression of cDNA encoding the human 150 kDa oxygen-regulated protein, ORP150". Biochem Biophys Res Commun. 230 (1): 94–9. doi:10.1006/bbrc.1996.5890. PMID9020069.
^Ozawa K, Kuwabara K, Tamatani M, Takatsuji K, Tsukamoto Y, Kaneda S, Yanagi H, Stern DM, Eguchi Y, Tsujimoto Y, Ogawa S, Tohyama M (Mar 1999). "150-kDa oxygen-regulated protein (ORP150) suppresses hypoxia-induced apoptotic cell death". J Biol Chem. 274 (10): 6397–404. doi:10.1074/jbc.274.10.6397. PMID10037731.
Kuznetsov G, Chen LB, Nigam SK (1997). "Multiple molecular chaperones complex with misfolded large oligomeric glycoproteins in the endoplasmic reticulum". J. Biol. Chem. 272 (5): 3057–63. doi:10.1074/jbc.272.5.3057. PMID9006956.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID9373149.
Tsukamoto Y, Kuwabara K, Hirota S, et al. (1998). "Expression of the 150-kd oxygen-regulated protein in human breast cancer". Lab. Invest. 78 (6): 699–706. PMID9645760.
Bando Y, Ogawa S, Yamauchi A, et al. (2000). "150-kDa oxygen-regulated protein (ORP150) functions as a novel molecular chaperone in MDCK cells". Am. J. Physiol., Cell Physiol. 278 (6): C1172–82. PMID10837345.
Kaneda S, Yura T, Yanagi H (2000). "Production of three distinct mRNAs of 150 kDa oxygen-regulated protein (ORP150) by alternative promoters: preferential induction of one species under stress conditions". J. Biochem. 128 (3): 529–38. doi:10.1093/oxfordjournals.jbchem.a022783. PMID10965054.
Tamatani M, Matsuyama T, Yamaguchi A, et al. (2001). "ORP150 protects against hypoxia/ischemia-induced neuronal death". Nat. Med. 7 (3): 317–23. doi:10.1038/85463. PMID11231630.
Zhang H, Li XJ, Martin DB, Aebersold R (2003). "Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry". Nat. Biotechnol. 21 (6): 660–6. doi:10.1038/nbt827. PMID12754519.
Bando Y, Tsukamoto Y, Katayama T, et al. (2005). "ORP150/HSP12A protects renal tubular epithelium from ischemia-induced cell death". FASEB J. 18 (12): 1401–3. doi:10.1096/fj.03-1161fje. PMID15240565.
Ahmed M, Forsberg J, Bergsten P (2005). "Protein profiling of human pancreatic islets by two-dimensional gel electrophoresis and mass spectrometry". J. Proteome Res. 4 (3): 931–40. doi:10.1021/pr050024a. PMID15952740.
Lewandrowski U, Moebius J, Walter U, Sickmann A (2006). "Elucidation of N-glycosylation sites on human platelet proteins: a glycoproteomic approach". Mol. Cell Proteomics. 5 (2): 226–33. doi:10.1074/mcp.M500324-MCP200. PMID16263699.
Cechowska-Pasko M, Bankowski E, Chene P (2006). "The effect of hypoxia on the expression of 150 kDa oxygen-regulated protein (ORP 150) in HeLa cells". Cell. Physiol. Biochem. 17 (1–2): 89–96. doi:10.1159/000091467. PMID16543725.
Takeuchi S (2007). "Molecular cloning, sequence, function and structural basis of human heart 150 kDa oxygen-regulated protein, an ER chaperone". Protein J. 25 (7–8): 517–28. doi:10.1007/s10930-006-9038-z. PMID17131193.
Kitao Y, Matsuyama T, Takano K, et al. (2007). "Does ORP150/HSP12A protect dopaminergic neurons against MPTP/MPP(+)-induced neurotoxicity?". Antioxid. Redox Signal. 9 (5): 589–95. doi:10.1089/ars.2006.1518. PMID17330988.