|PDB structures||RCSB PDB PDBe PDBsum|
|Gene Ontology||AmiGO / QuickGO|
- haloacetate + H2O glycolate + halide
This enzyme belongs to the family of hydrolases, one of the largest known enzyme families comprising approximately 1% of the genes in the human genome, exists as a homodimer, and acts specifically halide bonds in carbon-halide compounds. The systematic name of this enzyme class is haloacetate halidohydrolase. This enzyme is also called monohaloacetate dehalogenase and fluoroacetate dehalogenase. This enzyme participates in gamma-hexachlorocyclohexane degradation and 1,2-dichloroethane degradation.
is unique because it catalyzes the cleavage of the remarkably stable carbon–fluorine bond of a fluorinated aliphatic compound. In the reaction of L-2-haloacid dehalogenase and fluoroacetate dehalogenase, the carboxylate group performs a nucleophilic attack on the alpha-carbon atom, moving the halogen atom. This action is common to haloalkane dehalogenase and 4-chlorobenzoyl-CoA dehalogenase. DL-2-Haloacid dehalogenase is unique in that a water molecule directly attacks the substrate, displacing the halogen atom.
As fluoroacetate is poisonous and present in plants endemic to Australia, Africa, and Central America, livestock are often killed by fluoroacetate poisoning. Fluoroacetate is lethal to sheep and cattle at doses of 0.25 to 0.5 mg/kg of body weight, and is a problem in the livestock industry. A fluoroacetate dehalogenase gene from the soil bacterium Moraxella species strain B was transferred into the rumen bacterium Butyrivibrio fibrisolvens and expressed in vitro at sufficiently high levels to detoxify fluoroacetate in the surrounding medium. Scientists and farmers want to determine a way to get B. fibrisolvens into either the animals or plants.
- GOLDMAN P (1965). "THE ENZYMATIC CLEAVAGE OF THE CARBON-FLUORINE BOND IN FLUOROACETATE". J. Biol. Chem. 240: 3434–8. PMID 14321384.
- Goldman P, Milne GW (1966). "Carbon-fluorine bond cleavage. II. Studies on the mechanism of the defluorination of fluoroacetate". J. Biol. Chem. 241 (23): 5557–9. PMID 5928195.
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- Tatsuo Kurihara Journal of Biochemistry. Vol. 131, pp. 671-677 (2002), Regular paper;2002
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