Har Gobind Khorana
|Har Gobind Khorana|
January 9, 1922|
Kabirwala, Punjab, British India (now Punjab, Pakistan)
|Died||November 9, 2011
Concord, Massachusetts, U.S.
|Residence||India, United States, United Kingdom|
University of Wisconsin, Madison (1960–70)
University of British Columbia (1952–60)
Cambridge University (1950–52)
Swiss Federal Institute of Technology, Zurich (1948–49)
|Alma mater||University of the Punjab (Panjab University)
University of Liverpool
|Known for||First to demonstrate the role of nucleotides in protein synthesis|
|Notable awards||Nobel Prize in Medicine (1968), Gairdner Foundation International Award, Louisa Gross Horwitz Prize, Albert Lasker Award for Basic Medical Research, Padma Vibhushan, Willard Gibbs Award|
Har Gobind Khorana, also called Hargobind Khorana, (January 9, 1922 – November 9, 2011) was an Indian-American biochemist who shared the 1968 Nobel Prize for Physiology or Medicine with Marshall W. Nirenberg and Robert W. Holley for research that helped to show how the order of nucleotides in nucleic acids, which carry the genetic code of the cell, control the cell’s synthesis of proteins. Khorana and Nirenberg were also awarded the Louisa Gross Horwitz Prize from Columbia University in the same year.
Khorana was born in Raipur, British India (today Tehsil Kabirwala, Punjab, Pakistan) and became a naturalized citizen of the United States in 1966, and subsequently received the National Medal of Science. He served as MIT's Alfred P. Sloan Professor of Biology and Chemistry, Emeritus and was a member of the Board of Scientific Governors at The Scripps Research Institute.
Ribonucleic acid (RNA) with three repeating units (UCUCUCU → UCU CUC UCU) produced two alternating amino acids. This, combined with the rachit and Leder experiment, showed that UCU codes for Serine and CUC codes for Leucine. RNAs with three repeating units (UACUACUA → UAC UAC UAC, or ACU ACU ACU, or CUA CUA CUA) produced three different strings of amino acids. RNAs with four repeating units including UAG, UAA, or UGA, produced only dipeptides and tripeptides thus revealing that UAG, UAA and UGA are stop codons.
With this, Khorana and his team had established that the mother of all codes, the biological language common to all living organisms, is spelled out in three-letter words: each set of three nucleotides codes for a specific amino acid. Their Nobel lecture was delivered on December 12, 1968. Khorana was the first scientist to chemically synthesize oligonucleotides.
He extended the above to long DNA polymers using non-aqueous chemistry and assembled these into the first synthetic gene, using polymerase and ligase enzymes that link pieces of DNA together, as well as methods that anticipated the invention of PCR. These custom-designed pieces of artificial genes are widely used in biology labs for sequencing, cloning and engineering new plants and animals, and are integral to the expanding use of DNA analysis to understand gene-based human disease as well as human evolution. Khorana's invention(s) have become automated and commercialized so that anyone now can order a synthetic gene from any of a number of companies. One merely needs to send the genetic sequence to one of the companies to receive an oligonucleotide with the desired sequence.
Since the middle of the 1970s, his lab has studied the biochemistry of bacteriorhodopsin, a membrane protein that converts light energy into chemical energy by creating a proton gradient. Later, his lab went on to study the structurally related visual pigment known as rhodopsin.
The University of Wisconsin-Madison, the Government of India (DBT Department of Biotechnology), and the Indo-US Science and Technology Forum jointly created the Khorana Program in 2007. The mission of the Khorana Program is to build a seamless community of scientists, industrialists, and social entrepreneurs in the United States and India.
The program is focused on three objectives: Providing graduate and undergraduate students with a transformative research experience, engaging partners in rural development and food, security, and facilitating public-private partnerships between the U.S. and India. In 2009, Khorana was hosted by the Khorana Program and honored at the 33rd Steenbock Symposium in Madison, Wisconsin.
- "HG Khorana Britannica".
- Caruthers, M.; Wells, R. (2011). "Har Gobind Khorana (1922-2011)". Science 334 (6062): 1511. doi:10.1126/science.1217138. PMID 22174242.
- Rajbhandary, U. L. (2011). "Har Gobind Khorana (1922–2011)". Nature 480 (7377): 322. doi:10.1038/480322a.
- "The Official Site of Louisa Gross Horwitz Prize".
- "MIT HG Khorana MIT laboratory".
- "HG Khorana Nobel Lecture".
- Khorana, H. G. (1979). "Total synthesis of a gene". Science 203 (4381): 614–625. doi:10.1126/science.366749. PMID 366749.
- Kleppe, K.; Ohtsuka, E.; Kleppe, R.; Molineux, I.; Khorana, H. G. (1971). "Studies on polynucleotides *1, *2XCVI. Repair replication of short synthetic DNA's as catalyzed by DNA polymerases". Journal of Molecular Biology 56 (2): 341–361. doi:10.1016/0022-2836(71)90469-4. PMID 4927950.
- Wildenauer, D.; Khorana, H. G. (1977). "The preparation of lipid-depleted bacteriorhodopsin". Biochimica et Biophysica Acta 466 (2): 315–324. doi:10.1016/0005-2736(77)90227-9. PMID 857886.
- Ahuja, S.; Crocker, E.; Eilers, M.; Hornak, V.; Hirshfeld, A.; Ziliox, M.; Syrett, N.; Reeves, P. J.; Khorana, H. G.; Sheves, M.; Smith, S. O. (2009). "Location of the Retinal Chromophore in the Activated State of Rhodopsin*". Journal of Biological Chemistry 284 (15): 10190–10201. doi:10.1074/jbc.M805725200. PMC 2665073. PMID 19176531.
- "Gobind Khorana, MIT professor emeritus, dies at 89 – MIT News Office". Web.mit.edu. 2011-11-07. Retrieved 2011-11-12.
- Chandigarh mourns death of Nobel laureate Hargobind Khorana
- The Khorana Program
- 33rd Steenbock Symposium
- Thomas P. Sakmar (2012). "Har Gobind Khorana (1922–2011): Pioneering Spirit". PLoS Biology 10 (2). doi:10.1371/journal.pbio.1001273.
- Remembering Har Gobind Khorana: University of Wisconsin Biochemistry Newsletter, adapted from article in Cell
- Har Gobind Khorana materials in the South Asian American Digital Archive (SAADA)