The Hawthorn Tram Depot was built in 1916 for the Hawthorn Tramways Trust (HTT). It was built on the corner of Power Street and Wallen Road, Hawthorn, a suburb of Melbourne, Victoria. The depot was close to the junction of the HTT's two main lines. It was taken over by the Melbourne and Metropolitan Tramways Board (M&MTB) in 1920. In 1925 the depot was used as a school to teach tram drivers and tram conductors. In 1940, the building was also used to make uniforms for M&MTB staff.
The depot closed as a running depot on 13 February 1965, but was kept in use for training and uniform manufacture until the 1990s. In 1996 the depot was added to the Victoria Heritage Register. It was redeveloped and reopened in 2002 as a residential complex. One of the tram sheds has been kept as the Melbourne Tram Museum @ Hawthorn Depot.
The tram museum is owned by Victrack, but the museum is staffed and run by the Friends of Hawthorn Tram Depot. This group is a volunteer, non-profit group set up to preserve the history of Melbourne's trams. The museum has 17 fully restored trams. The collection also includes one of Melbourne's original cable trams.