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Heartwood is wood nearer the pith of a stem or branch, different in colour from sapwood.
Heartwood may also refer to:
- Heartwood Forest, which, when complete, will be the largest new native forest in England
- Heartwood House, a Canadian charity
- Heartwood – The Southwest Virginia Artisan Gateway, a building in Virginia, United States
- Heartwood (film), a 1998 American film starring Jason Robards