It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity.
Hepatocyte growth factor has been shown to interact with the protein product of the C-Met oncogene, identified as the HGF receptor (HGFR).Both overexpression of the Met/HGFR receptor protein and autocrine activation of Met/HGFR by simultaneous expression of the hepatocyte growth factor ligand have been implicated in oncogenesis.
^Gallagher, J.T., Lyon, M. (2000). "Molecular structure of Heparan Sulfate and interactions with growth factors and morphogens". In Iozzo, M, V. Proteoglycans: structure, biology and molecular interactions. Marcel Dekker Inc. New York, New York. pp. 27–59.
^ abBottaro DP, Rubin JS, Faletto DL, Chan AM, Kmiecik TE, Vande Woude GF et al. (Feb 1991). "Identification of the hepatocyte growth factor receptor as the c-met proto-oncogene product". Science251 (4995): 802–4. doi:10.1126/science.1846706. PMID1846706.
^Yang ZJ, Zhang YR, Chen B, Zhang SL, Jia EZ, Wang LS et al. (Jul 2009). "Phase I clinical trial on intracoronary administration of Ad-hHGF treating severe coronary artery disease". Molecular Biology Reports36 (6): 1323–9. doi:10.1007/s11033-008-9315-3. PMID18649012.
^Hahn W, Pyun WB, Kim DS, Yoo WS, Lee SD, Won JH et al. (Oct 2011). "Enhanced cardioprotective effects by coexpression of two isoforms of hepatocyte growth factor from naked plasmid DNA in a rat ischemic heart disease model". The Journal of Gene Medicine13 (10): 549–55. doi:10.1002/jgm.1603. PMID21898720.
^Zhong A, Wang G, Yang J, Xu Q, Yuan Q, Yang Y et al. (Jul 2014). "Stromal-epithelial cell interactions and alteration of branching morphogenesis in macromastic mammary glands". Journal of Cellular and Molecular Medicine18 (7): 1257–66. doi:10.1111/jcmm.12275. PMID24720804.
Ware LB, Matthay MA (May 2002). "Keratinocyte and hepatocyte growth factors in the lung: roles in lung development, inflammation, and repair". American Journal of Physiology. Lung Cellular and Molecular Physiology282 (5): L924–40. doi:10.1152/ajplung.00439.2001. PMID11943656.
Funakoshi H, Nakamura T (Jan 2003). "Hepatocyte growth factor: from diagnosis to clinical applications". Clinica Chimica Acta; International Journal of Clinical Chemistry327 (1-2): 1–23. doi:10.1016/S0009-8981(02)00302-9. PMID12482615.
Skibinski G (2004). "The role of hepatocyte growth factor/c-met interactions in the immune system". Archivum Immunologiae Et Therapiae Experimentalis51 (5): 277–82. PMID14626426.
Hurle RA, Davies G, Parr C, Mason MD, Jenkins SA, Kynaston HG et al. (Oct 2005). "Hepatocyte growth factor/scatter factor and prostate cancer: a review". Histology and Histopathology20 (4): 1339–49. PMID16136515.
Kemp LE, Mulloy B, Gherardi E (Jun 2006). "Signalling by HGF/SF and Met: the role of heparan sulphate co-receptors". Biochemical Society Transactions34 (Pt 3): 414–7. doi:10.1042/BST0340414. PMID16709175.