Herbie Flowers began his musical training in 1956 when conscripted into the Royal Air Force: electing at first to serve for nine years as a bandsman playing tuba. He took up double bass as a second instrument to secure his "junior technician" stripe, and later moved to electric bass. After completing his military service he passed through the line-ups of several Dixieland jazz bands in the early 1960s, then discovered modern jazz. In 1965 he was engaged as a bandsman on the ocean liner Queen Elizabeth. In New York, after hearing a jazz electric bass in a night club, he acquired his own solid-body electric instrument, a Lake Placid Blue 1960 Fender Jazz Bass that he purchased from Manny's in New York City for $79.
In 1969 Flowers was a founding member of the group Blue Mink and played on their song "Melting Pot", which reached No. 3 in the UK Singles Chart. He was a member of CCS, and later featured in a mid-1970s line-up of T. Rex. In 1979, shortly after taking part in the annual A Song For Europe contest, performing "Mr Moonlight" with his group, "The Daisies", he became a co-founder of the band Sky, which had success in the United Kingdom and Australia.
Flowers is also known for having composed the novelty hit "Grandad" for Clive Dunn. According to Flowers, he came up with it after following an easy primer book on composing. All he needed was a hook, and he was struggling to come up with anything. He phoned a friend (Ken Pickett) who came round, ringing the doorbell, and the sound of the doorbell provided the hook he needed.
Perhaps Flowers' most famous bass line is the one he created for Lou Reed's "Walk on the Wild Side" from the album Transformer (1972), the only song by Reed to reach the Top 20 in the US.
Since Sky's demise in the early 1990s Flowers has spent most of his time playing jazz. He also worked as a bass guitar teacher at Ardingly College, and leads many "Rockshops" at schools, helping young people to create and perform their own songs, as well as covering many others. In 1998 session drummer Peter Boita again teamed up with Flowers to form the solid base of a rhythm section to set the words of poet Sir John Betjeman to music on a second album they recorded together. The resulting album was called Betjeman & Read (They’d previously worked together on the Poetry in Motion album ( released on Silhouette Records MDKR 1 which set the words of Sir John Betjeman to music ) which featured Boita and Flowers playing with a stellar line-up of artists including David Essex, Justin Hayward, Steve Harley, Donovan, Alvin Stardust, Captain Sensible and Annie Haslam amongst others with Beatles’ producer George Martin overseeing proceedings. Boita and Flowers reprised their roles when Poetry in Motion was performed live for a charity show at the Richmond Theatre on 5 April 1992.) Betjeman & Read was one of the last albums recorded at the RG Jones Recording studio in Wimbledon South London. The vocal artistes performing on this album include Cliff Richard, Marc Almond, Paul Young, Jon Anderson, Richard Sharp, Colin Blunstone, Gene Pitney, Leo Sayer, Donovan, Mike Read, The Rodolfus Choir and David Essex. In September 2009 Herbie founded a community choir, Shoreham Singers-by-Sea, which has in excess of 150 members; followed in September 2010 by the Ditchling Singers.