Histamine N-methyltransferase (HNMT, HMT) is an enzyme involved in the metabolism of histamine. It is one of two enzymes involved in the metabolism of histamine in mammals, the other being diamine oxidase (DAO). HNMT catalyzes the methylation of histamine in the presence of S-adenosylmethionine (SAM-e) forming N-methylhistamine. The HNMT enzyme is present in most body tissues but is not present in serum. Histamine N-methyltransferase is encoded by a single gene, HNMT, which in humans has been mapped to chromosome 2.
The function of the HNMT enzyme is histamine metabolism by ways of Nτ-methylation using SAM-e as the methyl donor, producing N-methylhistamine, which, unless excreted, can be further processed by monoamine oxidase B (MAOB) or by DAO. Methylated histamine metabolites are excreted with urine.
Whereas DAO comes to the blood stream from the organs where it is expressed (small bowel and large intestine ascendens, kidney, etc.) in a continuous manner and stored in plasma membrane-associated vesicular structures in epithelial cells, and therefore serum DAO activity can be reliably measured while diagnosing histamine intolerance, measurement of intracellular HNMT which presents primarily in the cells of the internal organs, like the liver, is troublesome, so diagnosis is done, as a rule, indirectly, through testing for genetic variants. Although the consequences of flawed DAO activity are often periodic, the consequences of flawed HNMT activity occur immediately, and the symptoms also immediately appear, for example, after meals.
The most studied genetic variant is T allele at rs11558538 (c.314C>T, p.Thr105Ile), a loss-of-function allele reducing HNMT activity and associated with diseases, typical for histamine intolerance, such as asthma, allergic rhinitis and atopic eczema (atopic dermatitis). Therefore the owners of this variant should avoid intake of HNMT inhibitors which hamper enzyme activity, and also should avoid intake of histamine liberators which release histamine from granules of mast cells and basophils. In a study of 48 adults, median enzyme activity was significantly lower in subjects with the CT or TT genotype than in those with the wild-type CC genotype (485 versus 631 U/mL of red blood cells). In another study of 195 subjects, the C314T variant also showed an association with serum Interleukin-8 (IL-8) levels — individuals with the CT or TT genotype had lower levels of IL-8 (1.2 ± 0.7 versus 2.1) and higher levels of histamine (107.0 ± 53.9 versus 85.6 ± 45.7 ng/mL) in comparison with individuals with the CC genotype. The carriers of CT and TT genotypes were merged in one group in this study because there were too few participants with TT to form a group large enough to make statistical relevance, therefore CT and TT might have different enzyme activity which is not yet studied as of 2020[update]. This effect may indicate that there may be a link between this genetic variant and inflammation. Although the relationship between histamine and IL-8 has not been fully studied as of 2020[update], it is known that histamine can increase the expression of IL-8 through H1 receptorsin vitro and enhance the release of IL-8 in different cell types.
Other genetic variants have been also identified to affect enzyme function. The rs1050891 (939A>G, 3′-UTR) variant leads to increased enzymatic activity (messenger RNA stability), while rs758252808 (c.179G>A, p.Gly60Asp) and rs745756308 (c.623T>C, p. Leu208Pro) lead to decreased enzymatic activity.
^ ab"HNMT Histamine N-methyltransferase". National Center for Biotechnology Information. Retrieved 30 November 2020. In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene. This article incorporates text from this source, which is in the public domain.