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ISAF may refer to:
- International Sailing Federation, the world governing body for Olympic and other competitive sailing
- International Security Assistance Force, the NATO-led security mission operating in Afghanistan since 2001
- Information Security Awareness Forum, an industry forum set up in 2008 by the British chapter of the Information Systems Security Association
- International Shark Attack File, a global database of shark attacks set up in the 1940s, now maintained by the American Elasmobranch Society
- International Symposium on Alcohol Fuels, an international bi-annual conference on alcohol and bio-fuels, established in 1976
- Independent States Allied Forces, a fictional military alliance in the 2001 video game, Ace Combat 04: Shattered Skies
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