He was born at Ferrara, and educated at the University of Padua. He was converted about 1540 to the Catholic faith through Cardinal Pole, but embraced Protestantism in the following year, and went to Strasbourg to teach Hebrew.
Owing to the Schmalkaldic War in Germany he was compelled to seek asylum in England, where he resided at Lambeth Palace with Archbishop Cranmer in 1547. In 1549 he succeeded Paul Fagius as Regius professor of Hebrew at Cambridge.
On the death of Edward VI of England he returned to Germany in 1553. At Zweibrücken he was imprisoned as a Calvinist. He became professor of Old Testament at the University of Heidelberg in 1561, and remained there until he was released from his post in 1577. He ultimately found refuge at the College of Sedan, where he died. According to Morison, he "when dying reversed his nation's decision, and exclaimed, Not Barabbas, but Jesus! (Vivat Christus, et pereat Barabbas!)."
His chief literary work was a Latin translation of the Bible from the Hebrew and Syriac. The New Testament translation, by Theodore Beza, appeared in 1569, at Geneva. The five parts relating to the Old Testament were published at Frankfurt between 1575 and 1579, in London in 1580, and in numerous later editions. The work was joint with Franciscus Junius (the elder), his son-in-law. Harris Fletcher remarks that there were two quite different versions of Tremellius available in the late 1500s:
The Junius-Tremellius Bible first appeared from 1575-79, and subsequently in two different major forms. One of these in 1585 was printed as a tall folio with copious marginal notes, which were for the greater part written by Tremellius. The folio editions contained, in addition to Tremellius' Latin Old Testament with this large amount of marginal notation, a complete Latin translation of the Apochrypha done by Junius, and two Latin translations of the New Testament, one being of the fragmentary Syriac version by Tremellius, and the other from the Greek by Beza. The other form in which this Bible appeared was printed, usually in quarto, without notes, with the Apochrypha, and after 1585 with only Beza's translation of the New Testament.
The Tremellius translation was favored by John Milton, "undoubtedly" the folio version, with Tremellius's marginal notes, according to Harris. It was used also by John Donne for his version of Lamentations. Archbishop James Ussher also used the Junius-Tremellius translation when compiling his Annals of the World.
- Nicholas Barker, 'The Perils of Publishing in the Sixteenth Century: Pietro Bizari and William Parry, Two Elizabethan Misfits', in Edward Chaney and Peter Mack (editors), England and the Continental Renaissance: Essays in Honour of J. B. Trapp (1990), pp. 125-6.
- James Morison, A Practical Commentary on the Gospel According to St. Matthew, (London: Hodder and Stoughton, 1902), p. 581.
- Fletcher, -Harris (1927). "Milton's Use of Biblical Quotations". The Journal of English and Germanic Philology. 26 (2): 145–165. JSTOR 27703025.
- Hunter, William B.; Shawcross, John T., eds. (1981). Milton Encyclopedia. p. 88.
- Jeffrey, David L. (1992). A Dictionary of Biblical Tradition in English. p. 433.
- James Ussher, Larry and Marion Pierce (editors), The Annals of the World, Revised and Updated from the 1658 English Translation, Master Books, Green Forest, AR (2003), p. 6
- Kenneth Austin (2007), From Judaism to Calvinism: The Life and Writings of Immanuel Tremellius (c. 1510-1580)
- Dagmar Drüll, Heidelberger Gelehrtenlexikon 1386-1651, Berlin: Springer, 2002, pp. 532–533.
- W. Becker: Immanuel Tremellius, ein Proselytenleben im Zeitalter der Reformation, 1890
- Works by Immanuel Tremellius at Post-Reformation Digital Library
- Erich Wenneker (1997). "Tremellius, Immanuel". In Bautz, Traugott. Biographisch-Bibliographisches Kirchenlexikon (BBKL) (in German). 12. Herzberg: Bautz. cols. 444–448. ISBN 3-88309-068-9.
- Literaturliste im Online-Katalog der Staatsbibliothek zu Berlin
- Schaff-Herzog Encyclopedia of Religious Knowledge, vol. 11, pp. 504.
- Immanuel Tremellius at the Mathematics Genealogy Project