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In vivo (Latin for "within the living") is experimentation using a whole, living organism as opposed to a partial or dead organism, or an in vitro ("within the glass", i.e., in a test tube or petri dish) controlled environment. Animal testing and clinical trials are two forms of in vivo research. In vivo testing is often employed over in vitro because it is better suited for observing the overall effects of an experiment on a living subject. The maxim in vivo veritas ("in a living thing [there is] truth") is used to describe this type of testing and is a play on in vino veritas, ("in wine [there is] truth") a well-known proverb.
In vivo vs. ex vivo research
In microbiology in vivo is often used to refer to experimentation done in live isolated cells rather than in a whole organism, for example, cultured cells derived from biopsies. In this situation, the more specific term is ex vivo. Once cells are disrupted and individual parts are tested or analyzed, this is known as in vitro.
Methods of use
According to Christopher Lipinski and Andrew Hopkins, "Whether the aim is to discover drugs or to gain knowledge of biological systems, the nature and properties of a chemical tool cannot be considered independently of the system it is to be tested in. Compounds that bind to isolated recombinant proteins are one thing; chemical tools that can perturb cell function another; and pharmacological agents that can be tolerated by a live organism and perturb its systems are yet another. If it were simple to ascertain the properties required to develop a lead discovered in vitro to one that is active in vivo, drug discovery would be as reliable as drug manufacturing."
- Life Science Technologies, Cell Signaling: In Vivo Veritas, Science Magazine, 2007
- Lipinski C, Hopkins A (2004). "Navigating chemical space for biology and medicine". Nature 432 (7019): 855–61. doi:10.1038/nature03193. PMID 15602551.