Costelloe was from Ballyduff, Tralee, County Kerry. He was nominated as a candidate to the Industrial and Commercial Panel of the Seanad by the Taoiseach, Seán Lemass following the death of Fianna Fáil senator Daniel Moloney. At the by-election, on 28 November 1963, Costelloe was elected on the first count with 112 votes as opposed to 87 for his Fine Gael opponent John Conlan (in a Seanad panel by-election, the electorate consists of the sitting deputies and senators).
Writing in The Irish Times under the pseudonym Backbencher, journalist John Healy claimed that Costelloe's election was due to the "Kerry Mafia" in the Fianna Fáil party who had insisted that Moloney, a Kerry native, should be replaced by someone from the same area. A much younger candidate had been proposed but was outvoted; in Healy's view this was through the influence of long-serving North Kerry TD Tom McEllistrim who hoped his son, also Tom McEllistrim would inherit his seat on his retirement, and wanted to ensure no other candidate could use the Seanad seat to build support in the constituency.
Healy, suggesting Costelloe was older than his claimed age of 63, and was poorly qualified for the Industrial and Commercial panel ("He runs a tidy little grocery shop and he has a bit of land."), pointed out that he had previously failed to be elected to Kerry County Council, and suggested Costelloe would have little to contribute in the Seanad ("he will cause the shorthand takers no trouble"). In the event, he made just four interventions during his term.
He was not re-elected at the 1965 Seanad election.
- John Healy ("Backbencher"), Inside Politics, The Irish Times, 30 November 1963.
- The sources are ambiguous about the name of the victor of the Seanad Industrial and Commercial Panel by-election on 28 November 1963. The Oireachtas members database list his name as "John Costello", but the record of Seanad proceedings names the new senator as "John Costelloe", and the latter spelling is also used in the record of the first Seanad division in which he voted, and in subsequent proceedings.
- Two Elected in By-Elections to the Senate, The Irish Times, 29 November 1963.
- "Mr. John Costelloe". Oireachtas Members Database. Retrieved 2 September 2014.
- Oireachtas listing of Costelloe's Seanad contributions