The museum was established on February 2, 1978, 4 years after the discontinued operation of the actual railway, when the specifically established joint stock company Jokioinen Museum Railway Ltd. (Jokioisten Museorautatie Oy) bought the Minkiö-Jokioinen rail line with its land and associated buildings. Museum steam trains began running that same year. In 1994 the line was extended with an 8 km stretch between Minkiö and Humppila.
The museum railway station at Humppila is beside the station of the Turku-ToijalaVR line, providing a convenient access to the museum.
The Minkiö station has a narrow gauge museum with a collection of carriages and locomotives. There are a number other attractions in the vicinity of the railway.