Kinyoun stain

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The Kinyoun method, or Kinyoun stain, is an acid-fast procedure used to stain any species of the genus Mycobacterium and Nocardia[1] species. It involves the application of a primary stain (carbol fuchsin), a decolorizer (acid-alcohol), and a counterstain (methylene blue).[2] Unlike the Ziehl-Neelsen stain (Z-N stain), the Kinyoun method of staining does not require heating.[3][4] In the Ziehl-Neelsen stain, heat acts as a physical mordant while phenol (carbol of carbol fuschin) acts as the chemical mordant. Since the Kinyoun stain is a cold method (no heat applied), the concentration of carbol fuschin used is increased.[5]

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  1. ^ Geo F. B et al, Jawetz, Melnick and Adelberg's Medical Microbiology, 25th Edition, Lange Medical,2004, page 182
  2. ^ Hussey, A. M., Zayaitz, A., "Acid-Fast Stain Protocols", American Society for Microbiology, 8 September 2008. Retrieved on 1 November 2014.
  3. ^ Murray PR, Baron E, Pfaller M, Tenover F, Yolken, Eds. Manual of clinical microbiology. 7th ed. Washington, DC: ASM, 1999.
  4. ^ Baron EJ, Finegold SM. Bailey and Scott's diagnostic microbiology. 8th ed. St. Louis: Mosby, 1990.
  5. ^ Ananthanarayan and Panicker's Textbook of Microbiology, 9th Edition, Universities Press (India), 2013, page 353