The airline was established and started operations in 1982. It was part of a full service safari company with its own lodges, camps, guides, aircraft and sailing vessels and operated services to remote destinations in Venezuela, including tourist attractions. It had 300 employees at March 2010. As of June 2018, the airline ceased all serves and plans to restart operations.
On April 17, 2009, an Línea Turística Aereotuy Cessna 208 Caravan aircraft crashed shortly after taking off from Canaima National Park after failing to gain sufficient altitude, killing one person. The aircraft had been chartered to take holiday makers to Ciudad Bolívar.