In addition to O'Brien serving as host, comedian Andy Richter serves as the show's announcer, and as a sidekick to O'Brien.Jimmy Vivino and the Basic Cable Band, formerly known as "The Max Weinberg 7" and briefly as "Max Weinberg and The Tonight Show Band", serves as the house band. The show follows the established six-piece format used during Conan O'Brien's tenure as host of The Tonight Show. The first two segments includes a monologue by O'Brien, sometimes accompanied by altered news clips, or several brief comedy sketches. Interview with two guests will likely follow, as well as a musical or comedy performance.
Unusual for a talk show, the titles for each episode are often offbeat in nature. However, since January 29, 2014, the titles have been removed due to a shortened intro.