List of Spider-Man supporting characters
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- 1 Family
- 2 Love Interests
- 3 Peter Parker's supporting characters
- 4 Allies
- 5 Enemies
- 6 Alternative continuities
- 7 Supporting characters in other media
- 8 Enemies in other media
- 9 References
- 10 External links
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- Richard Parker (father)
- Mary Parker (mother, née Fitzpatrick, deceased)
- Teresa Parker (sister): Peter Parker's long lost sister who is introduced in the graphic novel Spider-Man: Family Business
- Ben Parker (uncle, deceased)
- May Parker (aunt, née Reilly): Peter Parker's loving aunt, who raises him after his parents died. After the murder of her husband, Ben (Peter's uncle), May is virtually his only family, and they are very close.
- Zoe Parker (cousin): The history of the character is unknown
- Mary Jane Watson-Parker (former wife): originally merely Gwen Stacy's competition, M.J. eventually became Peter's best friend and wife. After Peter and Mary Jane made a deal with Mephisto to save Aunt May's life, their marriage is erased from official continuity.
- May Parker (daughter from MC2 universe- Earth-982)
- Benjy Parker (son from MC2 universe- Earth-982)
- Will Fitzpatrick (grandfather, deceased): Mary Parker's father and Peter Parker's grandfather
- Ben Reilly a.k.a. Scarlet Spider, and the second Spider-Man (clone brother, Deceased)
- Kaine Parker a.k.a. the second Scarlet Spider(clone brother)
- Spidercide (clone brother, deceased)
- Jack 
- Guardian 
- skeleton of a Spider-Man clone found in a smokestack
- Doppelganger (mystic duplicate)
- Jessica Drew a.k.a. Spider-Woman, Black Widow, Ultimate Spider-Woman, Ultimate Black Widow (clone, Sister) she is his clone in the Ultimate Universe (Earth 1610), but in the Marvel Universe (Earth 616) she is not his clone
Mary Jane's family
- Anna Watson: Mary Jane's aunt and Aunt May's best friend.
- Madeline Watson (mother, deceased)
- Phillip Watson (father)
- Kristy Watson (cousin)
- Gayle Watson-Byrnes (sister)
May Parker's Family
- Albert Reilly (father)
- Claire Reilly (mother)
- Horace Reilly (uncle)
- Bill Reilly (uncle)
- Claudia Reilly (aunt): Bill Reilly's wife
- Sam Reilly (cousin): Bill and Claudia's son
- Julia Reilly (cousin): Bill and Claudia's daughter (married last name unrevealed)
- Jan Reilly (sister)
- April Reilly (sister)
- Alexa (niece): Julia Reilly's daughter and Peter Parker's cousin, last name unrevealed
- Ames (niece): Peter Parker's cousin, last name unrevealed
- Amanda (niece): Peter Parker's cousin, last name unrevealed
Peter Parker's love interests
- Gwen Stacy: Gwen was Peter's first serious girlfriend. She was very kind but slightly spoiled, smart, beautiful and shared Peter's love for science. Her father was police Captain George Stacy. Peter initially ignored her due to his concern for his sick Aunt May, which frustrated Gwen. First a friendship, then a romance gradually formed between the two, which lasted for over a year, until her death. She was killed by the Green Goblin when he threw her off a bridge. In House of M, Gwen is still alive and married to Peter.
- Mary Jane Watson: listed above. Formerly depicted as Gwen Stacy's competition, she eventually became Peter's main love interest after Gwen (who Mary Jane was best friends with) died. She was really in love with Peter and even got married to him (before Mephisto altered reality).
- Black Cat a.k.a. Felicia Hardy: Peter's first superhuman girlfriend. She is calculating, strong and sly. Felicia competed against Mary Jane for Peter's affections, eventually losing after she left town for a while. She also dated Flash Thompson. She would continue on as an occasional love interest of Peter in some continuities.
- Anna Maria Marconi: Girlfriend of Peter when he was possessed by Dr. Octopus. Anna Maria met Peter as a student at Empire State University. The two hit it off and started dating. They started living together, and the relationship got so serious that Anna Maria found an engagement ring hidden in their apartment. After Peter regained control of himself, Anna became his friend.
- Betty Brant: Betty takes over her mother's former position as Daily Bugle secretary after she dies. Peter dates Betty Brant for a while, but they break up due to her blaming Spider-Man for the death of her brother. She later marries Daily Bugle reporter Ned Leeds, although she briefly gets back with Peter after the breakdown of her marriage. Despite this, both Betty and Peter maintain a close friendship.
- Black Widow a.k.a. Natasha Romanova: During a HYDRA attempt to take over S.H.I.E.L.D., she is tortured to such an extent that she regresses back to an old cover identity of schoolteacher Nancy Rushman, but she is recovered by Spider-Man in time to help Nick Fury and Shang-Chi work out what had happened and restore her memory, with "Nancy" developing an attraction to Spider-Man before her memory is restored during the final fight against Madam Viper, Boomerang and the Silver Samurai.
- Carlie Cooper: She is an officer of the NYPD's Crime Scene Unit and ex-best friend of Harry Osborn's ex-girlfriend, Lily Hollister. She had also been friends with Gwen Stacy. At Harry Osborn's goodbye party Peter asks her to be his girlfriend and the two share their first kiss. However they break up after Spider Island due to surmising that Peter was Spider-Man, and was angry that he'd lied to her. Carlie eventually left New York for her own safety.
- Debra Whitman: a fellow Empire State University student and secretary whom Peter dates for a period of time, though his frequent disappearances complicate their relationship. She is eventually diagnosed with mild schizophrenia, ironically exacerbated by her "delusional" belief that Peter is Spider-Man. With Peter's help, she overcomes that idea. Soon afterward, she leaves New York after another man, Biff Rifkin, confesses his strong feelings for her.
- Jean DeWolff: In a situation that never turned into anything physical or... requited, Spider-Man learns that police chief Jean Dewolff had a longtime crush on the web slinger. Unfortunately, she was killed by the Sin Eater when Spidey leapt out of the way of a shotgun blast.
- Julia Carpenter: They flirted with one another. She made his black costume (the non-symbiote version).
- Liz Allan: Peter's high school crush, although they never get together. Liz instead marries Peter's friend, Harry Osborn, who's divorced now.
- Marrow a.k.a. Sarah: A brainwashed Marrow is convinced she is an Empire State University student named Sarah Rushman. She develops a crush on her lecturer, Peter Parker, and the two go on a couple of dates before she realizes who she is.
- Michele Gonzales: Michele is a criminal defense lawyer and the hot-tempered sister of Peter's roommate Vin Gonzales, as well his temporary roommate while Vin serves time for his involvement in the Spider-Tracer Killings frame-up (to which she got him a plea bargain). When Michelle attempts to kick Peter out (who is actually the Chameleon in disguise) she is instead seduced by him and become infatuated with him. She is almost constantly angry, and questioning her or drawing attention to the size of her buttocks really sets her off. However, she is also a helpful and kind person, by trying hard to help her clients get their lives back on track. After pestering and bothering Peter tirelessly, she returned to her previous home in Chicago shortly after Vin's release.
- Ms. Marvel: Carol Danvers, the superhero Ms. Marvel, has worked with Spider-Man on occasion and even agreed to go on a date with him in accordance with his helping her on a mission, despite how angry he can make her. She later fulfills her promise. Spider-Man had admitted to himself he finds her attractive in her outfit. At the end of the near disastrous date, the two bonded together over a love of junk food. After she was possessed by the symbiote for a time, Venom hints to Spider-Man that his feelings for Ms. Marvel are mutual. The two have remained good friends.
- Silk a.k.a. Cindy Moon: In the Original Sin storyline, When Spider-Man was exposed to the energies of the Watcher’s eye, he recalled the first time the spider that bit him, but not before the radioactive spider that bit him managed to bite another before it died, Cindy Moon. Cindy shows remarkable abilities that are quicker and faster than Peter's. She felt a primal connection to Peter as they show an animalistic attraction to one another, and that bond was seen in a more tender, caring way throughout the Spider-Verse series. However, Cindy seems determined to keep Peter at a distance, as though attempting to come to terms with her own identity without the input of her famous ally.
- Silver Sable: She has worked with Spider-Man on occasion. She shared a passionate moment with him when they stopped a bomb from exploding in Ney York City and greatly aided him during the Ends of the Earth.
- Emma Frost: In Marvel Adventures Spider-man, Emma reveals that she has a crush on Peter and tries to separate Chat and Spidey by erasing Chat's memory. On her own, she decides to pay for her crimes and get arrested, but returns to help Spidey, apparently, still holding a crush on Peter.
- Kitty Pryde: In the Ultimate Marvel Universe, Kitty Pryde of the X-Men had a crush on Spider-Man and would eventually become his girlfriend, though this did not last because after the clone saga Spider-man realized he still loves Mary Jane.
- Sophia "Chat" Sanduval: A mutant known as Chat from the Marvel Adventures Spider-Man who appeared in issue 53 and is best friends with this version's Emma Frost. She has the power to talk to animals. She is one of the few people who knows Spider-Man's identity and has developed deep feelings for him. She and Peter begin dating, meanwhile Emma (with her own crush on Peter) shakes their relationship. Eventually, Chat becomes Peter's girlfriend.
- Jean Grey: When the X-Men save the life of Spider-Man by using some freshly drawn enzymes from Morbius and Professor Xavier's equipment, Spidey shows his gratitude by kissing Jean.
- Rogue: In X-Men Forever continuity, Spider-Man helped Rogue when she was annoyed with events happening around herself, her new powers and the X-Men's status. Rogue somewhat takes Spider-Man's advice to follow her heart and, now that she can touch people, almost kisses him.
- Silver Sable: In the What If? storyline "What If Spider-man had married the Black Cat?", after Black Cat is accidentally killed by the Paladin, Silver Sable and Spider-Man enter into a romantic relationship due to their shared grief over the loss of their loved ones; Uatu the Watcher leaves it ambiguous whether this relationship will work out or not. Incidentally, the 616 version of Sable confessed to have romantic interest towards Spider-man.
Ben Reilly's love interests
- Janine Godbe a.k.a. Elizabeth Tyne The one great love of Ben's life. Ben found out Janine was living under an assumed name after she murdered the father who molested her. Ben confided in Janine that he was a clone of Spider-Man. Later Kaine forced Janine to fake her death to hurt Ben. Janine later turned herself into the police for killing her father. In the MC2 universe Darkdevil is Ben and Janine's son.
- Betty Brant was attracted to the Scarlet Spider during the Web of Life storyline where Ben saved her from the Grim Hunter.
- Jessica Carradine is the daughter of the burglar who killed Peter Parker's uncle Ben. She dated Ben during the period where he was Spider-Man and thought he was the real Peter Parker. Janine is a photographer and discovers Ben is Spider-Man.
- Firestar: Ben and Firestar had an attraction towards each other, but it was never acted upon.
- Lois Lane: When Ben competed in Marvel vs DC he met Lois Lane and asked her on a date to find out she is engaged to Clark Kent/Superman. Lane, however, develops a slight attraction towards Ben's alter-ego Spider-Man before their mutual universes are separated again.
- Carrie Bradley: A woman Ben was supposed to go on a date with but failed to make the date on time because of his life as a super hero.
- Black Cat: They both dated when Ben was acting as Spider-Man. He admitted that he was not the Spider-Man she knew, but he never revealed his true identity.
Ben had Peter's memories of dating Betty Brant and Gwen Stacy, though not Mary Jane. This is due to the fact that Peter had not yet developed feelings for Mary Jane, at the time he was cloned.
Peter Parker's supporting characters
- Jean DeWolff: A police captain and good friend of Spider-Man. She fell in love with Spider-Man (rather than Peter Parker, as she never learned his true identity), and eventually confessed those feelings to him during a time when he was physically asleep and his body being manipulated by the Venom symbiote. She eventually died in the line of duty, with Peter never knowing that she had had feelings for him, though he did have suspicions after finding a collection of photographs of the two of them together after her death.
- Flash Thompson: Peter's former high school bully and later best friend.
- Ashley Kafka: A psychiatrist at Ravencroft Institute for the criminally insane who has occasionally sided with Spider-Man against super-villains.
- Randy Robertson: Joe Robertson's son. Friend of Peter Parker.
- Sha Shan: Former lover of Flash Thompson; written out of stories between "Amazing Spider-Man" issues 280 and 622 due to anachronistic nature of the characters origins.
- Dexter Bennett
- Lily Hollister
- Sally Avril
- Jimmy-6 a.k.a. Giacomo Fortunato
- Leo Zelinsky
- Vin Gonzales
- Ned Leeds
- J. Jonah Jameson: Peter Parker's boss at the Daily Bugle and its publisher. He has a campaign to discredit Spider-Man..
- Betty Brant: J. Jonah Jameson's former secretary. Later becomes a reporter for the Daily Bugle.
- Glory Grant: Former neighbor and friend of Peter Parker. Later becomes J. Jonah Jameson's secretary.
- Joe "Robbie" Robertson: A high-ranking editor at the New York newspaper The Daily Bugle and a close friend and confidant of publisher J. Jonah Jameson, acting as a voice of reason in Jameson's campaign to discredit Spider-Man.
- Norah Winters: A reporter for the Daily Bugle.
- Ben Urich: Urich is a chain-smoking, tough-as-nails investigative journalist for the New York newspaper The Daily Bugle.
- Bella Fishbach: Bella is a co-worker of Peter Parker at Horizon Labs. Bella is part of the Lucky Seven. She is specialized in green-technology. She now works at Parker Industries.
- Grady Scraps: Grady is the comical co-worker of Peter Parker. He is, just like Peter, a member of the Lucky Seven think-tank at Horizon Labs. He now works for Parker Industires.
- Max Modell: Peter's boss in Horizon Labs after Marla Jameson recommended Max to hire him. After Horizon Labs is destroyed, he went on to work for Parker Industries.
- Sajani Jaffrey: Sajani, creator of an artificial Vibranium called Reverbium, works with Peter Parker at Horizon Labs where she is a member of the Lucky Seven. She later went on to work for Parker Industries.
- Uatu Jackson: Uatu is a pre-teen genius who works in Horizon Labs and is a member of the Lucky Seven.
- Anna Maria Marconi
- Bella Fishbach
- Cardiac: A physician and surgeon, and the owner and administrator of a biological research firm. Elias Wirtham is driven by his brother's death to research life-saving medical practices. He serves as an ally and occasional adversary of Spider-Man.
- Grady Scraps
- Living Brain: A robot was created by the fictional International Computing Machines Corporation and billed as the most intelligent computer and robot in existence, capable of solving virtually any question asked of it.
- Max Modell: Max Modell and his partner Hector Baez were brought in by Peter Parker to run Parker Industries west coast operations where they will be specifically running Horizon University.
- Sajani Jaffrey
Ben Reilly's supporting characters
- Gabrielle Greer: A young woman who lived at the same hotel as Ben, when he first returned to New York City.
- Shirley Lewis (née Washington): Owner of the Daily Grind and Ben's boss.
- Desiree Winthrop: A model and regular at the Daily Grind.
- Buzz: A regular at the Daily Grind (his real name was never stated). On a few occasions he helped Ben (not realizing he's Spider-Man), through useful advice.
- Devon Lewis: Son of Shirley and part-time employee at the Daily Grind. At first Devon was distrustful of Ben, but over the months that followed he warmed up to him.
Kaine's supporting characters
- Aracely: A 16-year-old Mexican girl, whom Kaine saved from human traffickers and took in, upon his arrival in Houston. It's later revealed that Aracely possesses psychic abilities, similar to Madame Web. Her full name is María Aracely Josefina Penalba de las Heras, and adopted the codename "Hummingbird"
- Annabelle Adams: A bartender/singer at the Four Seasons Kaine lives at. Annabelle is Mexican, which prompted Kaine to ask her for help with Aracely (who couldn't speak English). Kaine later attends a concert Annabelle's band holds.
- Donald Meland: A Houston doctor whom Kaine meets when he brings in the half-dead Aracely to the hospital.
- Wallence "Wally" Layton: A Houston police officer, who encouraged Kaine to continue as a vigilante in Houston. Wally is gay and married to Donald Meland.
Gwen Stacy's family
- George Stacy (deceased): Gwen Stacy's father, Police Captain. Introduced in The Amazing Spider-Man (vol. 1) #56 (1968). He approves of Peter and Gwen's relationship as boyfriend and girlfriend. During a fight between Spider-Man and Doctor Octopus, he is crushed by falling debris while saving a child. As he dies, he reveals to Peter that he had known his identity for some time (something Peter had suspected anyway), and asks Peter to take care of Gwen.
- Helen Stacy: Gwen's mother and wife of George. Her first and only appearance is Peter Parker: Spider-Man #-1 (minus one).
- Arthur Stacy: Gwen Stacy's uncle, a private investigator, first appeared in The Amazing Spider-Man (vol. 1) #93 and #95. He was reintroduced only in the 1990s, in Peter Parker: Spider-Man #70 (in the last part of 'Clone Saga'). He is George's younger brother, but was originally presented in the 1970s as the older brother. For a time, Spider-Man would call on Stacy's skills as an investigator.
- Nancy Stacy: Gwen's aunt. Wife of Arthur and mother of Jill and Paul. First appeared in The Amazing Spider-Man (vol. 1) #93.
- Jill Stacy: Arthur Stacy's daughter and Gwen's cousin. Friend of Mary Jane and Peter. After Mary Jane was kidnapped, and thought to be dead, Jill made romantic advances toward Peter. Introduced in Peter Parker: Spider-Man #76 (1997).
- Paul Stacy: Arthur Stacy's son and Gwen's cousin. He blames Spider-Man for Gwen's death and joins the anti-mutant group the Friends of Humanity in an attempt to bring him to justice. Introduced in The Amazing Spider-Man (vol. 1) #422.
- Gabriel : Gwen's son by Norman Osborn. Norman convinced Gabriel and his sister, Sarah, that Peter Parker was their father and had killed their mother. Although Sarah is persuaded otherwise, Gabriel continues to believe so and takes on the identity of the "Gray" Goblin. After a confrontation with Spider-Man, he crashed into the river on his glider and lost his memories. Sarah took him to their home in France to recover. After failing to convince Sarah to join him, he flies off on a different glider. Introduced in The Amazing Spider-Man #509.
- Sarah (last name unrevealed): Gwen's daughter by Norman Osborn. Norman convinced Sarah and her brother, Gabriel, that Peter Parker was their father and had killed their mother. Sarah becomes suspicious after she meets Spider-Man however. She is convinced of the truth when Spider-Man saves her life by giving her a blood transfusion after she is shot by police. Spider-Man later learns that the pain caused by her accelerated aging has led her to abuse painkillers, and her addiction has gotten her in trouble with the French authorities. However, she promises to seek help, and perhaps someday become a hero herself. Introduced in The Amazing Spider-Man #509.
- Anya Corazon
- Ezekiel Sims
- Julia Carpenter
- Madame Web
- Scarlet Spider (Ben Reilly)
- Scarlet Spider (Kaine Parker)
- Spider-Man (Miguel O'Hara) a.k.a. Spider-Man 2099
- Spider-Man (Miles Morales) a.k.a. Ultimate Spider-Man
- Spider-Man (Peter Parker) a.k.a Spider-Man
- Spider-Woman (Jessica Drew)
- Spider-Woman (Mattie Franklin)
Earth-928 a.k.a. Earth-616 circa 2099
Omniverse (No Main Universe or Multiverse)
House of M
Supporting characters in other media
These Supporting characters do not appear in the comics. They were created for various media series. Among them are:
- Anthony "Henry" Harper (appeared in The Amazing Spider-Man, voiced by David Lodge): Nothing much is known about District Attorney Henry Harper's past. In one side-mission in the game, he is kidnapped by Iguana and is dragged into the sewers through the train docking station. After saving a civilian, Spider-Man learns that Harper was a pawn for Oscorp. After Spider-Man defeats Iguana, Harper is rescued and he escapes out of the sewers. Sometime before the events of the game, Harper had focused on exposing Quest Aerospace's evil schemes after he successfully prosecuted some of the city's most notorious criminals. The corrupt corporation lost millions of dollars to Harper, but they fired back when they had evidence of funds contributed to the D.A.'s reelection campaign were sourced by Oscorp Industries. These allegations were never revealed, but Harper's reputation was severely damaged in the eyes of many citizens. He is not seen in The Amazing Spider-Man 2, but he is mentioned when Spider-Man tells the Shocker that he could get into protective custody in exchange for telling Harper about the gang war.
- Dr. Watts (appeared in Spider-Man 2: Enter Electro): Dr. Watts (first name unknown) is a prominent scientist. She is the creator of the Bio-Nexus Device and a world-renowned scholar of biology. In the game, she is kidnapped by Hammerhead while attending the Science Industry Ball. During Spider-Man's fight with Hammerhead, Dr. Watts disappears, which led Spider-Man to believe that the Sandman took her. Due to an anonymous tip, Spider-Man tracks Dr. Watts to a museum, where she is taken hostage by Electro. After Electro leaves the museum, Dr. Watts tells Spider-Man that Electro went to the Twin Towers.
- Detective Terri Lee (appeared in Spider-Man): She is a detective for the New York Police Department. Naturally, she investigates cases that involve Spider-Man and to an extent to Peter Parker, though she never found out Peter and Spider-Man are the same person. She didn't trust Spider-Man at first, but overtime she began accepting Spider-Man as an ally. She has ties to Carnage and she had a relationship with the vampire hunter Blade.
- Indira "Indy" Daimonji (appeared in Spider-Man: The New Animated Series): She is Peter Parker's potential love interest and a rival of Mary Jane Watson. She works at a news network down in Manhattan. She is seriously injured by Spider-Man in the series finale when Spider-Man is tricked by the Gaines Twins into injuring Indy. This led to Peter giving up being Spider-Man
- Whitney Chang (appeared in The Amazing Spider-Man and The Amazing Spider-Man 2, voiced by Claudia Black (ASM 1) and Sumalee Montano (ASM 2)): She is a top investigative reporter for the Channel 3 News Network and is well known for putting herself at risk for finding out the truth. She met Spider-Man in person while she was investigating a secret Oscorp facility involving cross-species genetics and its connection with Alistair Smythe, with Spider-Man tracking down a crate with Dr. Connor's research to create the cure. Whitney gave Spider-Man her camera and asks him to take photos exposing Oscorp's research. As explained in her bio, Chang grew up in the Sunset Park region of Brooklyn, New York, where she witnessed a neighbor being murdered by an angry mob after being framed for murder and being slandered by the news for days. After graduating from Yale, she quits her job as the host of a music video channel and snuck aboard a flight to Iraq, arriving just as American troops invade Baghdad. In The Amazing Spider-Man 2, through unspecified circumstances, Whitney leaves her job at the Channel 9 News Network and now works at the Daily Bugle. She works with Daily Bugle newcomer Peter Parker to expose Wilson Fisk as the Kingpin. Her role in Amazing Spider-Man 2 is lessened compared to its predecessor.
Enemies in other media
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These enemies do not appear in the comics. They were created for various media series. Among them are:
- Wolf Spider (appeared in Ultimate Spider-Man voiced by Christopher Daniel Barnes) - Wolf Spider is an evil alternate version of Spider-Man from a parallel dimension. He appeared as the primary antagonist in the four-part season four episode arc "Return to the Spider-Verse". He first appeared as a top lieutenant for the Lizard's army of vampires in a Victorian era of New York City. After the Lizard and his vampire army were defeated, Wolf Spider escapes as he possesses a piece of the Siege Perilous as well. He later arrived in the 1880s attempting to capture another piece, but was driven back by Doc Ock Holiday (the Western version of Doctor Octopus) and the Phantom Rider. As he secretly follows Spider-Man and Miles Morales, he arrives in the Noir universe, attempting to convince Mister Negative to help him in the quest for domination, but the latter declined and attacked him, forcing the Wolf Spider to retreat. He jumps into Miles Morales' universe, ambushing Spider-Man and Morales at the NYPD headquarters holding the latter's mother hostage. They, along with an alternate version of Spider-Woman, rescue her and defeat the Wolf Spider, but not before collecting his shard of the Siege Perilous. He evades capture and invades Miles Morales' home, where he reveals himself to be an evil version of Peter Parker. He explains that he received a piece of the Siege Perilous himself and he has killed every version of Peter Parker that was deemed heroic since wielding the shard, including a Miles Morales from his own universe. He lures the three heroes to his underwater lair, where he reveals his master plan: using the power of the Siege Perilous to absorb the life force of every surviving Spider-Man from every dimension. He completes his mission, but Spider-Man tricks him, telling him that the heroic, "pure" life energies from each Spider-Man has corrupted the Wolf Spider's evil dark life force. Unable to fight it off, the Wolf Spider was killed.
- Monster-Ock (appeared in Spider-Man and Ultimate Spider-Man, voiced by Marcus Shirock (Spider-Man) and Fred Tatasciore (Ultimate Spider-Man): Monster-Ock is the name of the bonding between Spider-Man villains Doctor Octopus and Carnage. Initially, he was an exclusive villain to the 2000 video game Spider-Man. In his first appearance when Spider-Man defeats Carnage by using sonic weaponry, the symbiote left Cletus Kasady and it bonds onto an unconscious Doctor Octopus and Monster-Ock attempts to kill Spider-Man. After a lengthy chase throughout the underwater base, Spider-Man barely escapes and the symbiote left Octavius, leaving him catatonic, but alive. Spider-Man saves Octavius and the symbiote's, as well as Kasady's, fates were unrevealed. Years later, in Season 4 of Ultimate Spider-Man (titled Ultimate Spider-Man vs. The Sinister Six), a different version of Monster-Ock existed. In its only appearance in the animated series, '"The Symbiote Saga, Part One", Morbius bonded Doctor Octopus (whose body was morphed via nanites) with the Carnage symbiote as retaliation for Doc Ock transforming into a bat-like vampire. As Morbius escapes capture from Spider-Man and Agent Venom, Monster-Ock was preparing to kill Flash Thompson (whose Venom symbiote was struggling to stand up against the new symbiote threat) until Spider-Man used sonic weaponry to separate Doc Ock and the Carnage symbiote. An unconscious Doc Ock was webbed up by Spider-Man.
- Hyper-Electro (appeared in Spider-Man 2: Enter Electro) - Hyper-Electro is a powered-up version of the villain Electro and is exclusive to the game. In Enter Electro, Electro teams up with various villains in an attempt to retrieve pieces of the Bio-Nexus Device, a device designed to amplify one's bio-energy. He kidnaps Dr. Watts (the creator of the Bio-Nexus Device) and holds her hostage at a museum in exchange for Zeus' Tear (a rare sapphire that Spider-Man captured a short time before Electro arrived at the museum). After injuring Spider-Man, Electro connects the jewel to the Bio-Nexus Device and transforms into Hyper-Electro, a being of pure energy. In the end, an injured Spider-Man defeats Hyper-Electro by overloading the generators supporting Hyper-Electro's power and destroying the Bio-Nexus Device. Electro is last seen in prison sharing a cell with Hammerhead and Shocker.
- Rattan (appeared in Spider-Man and Captain America in Doctor Doom's Revenge): He was created specifically for the game.
- Nattie (appeared in The Amazing Spider-Man): A piranha-like creature as a result of Alistair Smythe's cross-species experiment. He was created specifically created for the game.
- Talon (appeared in Spider-Man: The New Animated Series, voiced by Eve): She is a villain created exclusively for the show. She bears many characteristics to the Black Cat. In her first and only appearance, "Keeping Secrets", she is behind a string of robberies, much to Spider-Man's dismay, who is a little concerned for her safety. Her real name is Cherry Valentine, and she is Harry Osborn's girlfriend. Peter learns about this, but he keeps this a secret to both Mary Jane and Harry. During a later encounter, she is shot by the police and she is seemingly killed. At the end of the episode, it was revealed she was alive and well, and she breaks up with Harry.
- Black Cat 2099 (appeared in Spider-Man: Edge of Time, voiced by Katee Sackhoff): She is a clone of the original Black Cat. It was revealed that she was upgraded with cybernetic weaponry and she claimed that Alchemax was forcing her to use anti-aging drugs to mislead Spider-Man. Black Cat 2099 also has clones of herself.
- Walker Sloan (appeared in Spider-Man: Edge of Time, voiced by Val Kilmer): Walker Sloan is one of Alchemax's greatest minds, and he serves as one of the main villains in the game. In the game, he travels back in time to establish Alchemax sometime in the 1970s, which results in Spider-Man's untimely death by the hands of Anti-Venom, who is revealed to be under mind control. Sloan was revealed to be working with Doctor Octopus to alter reality, much to Octavius' dismay. After Anti-Venom was freed from mind control, he prepares to kill Sloan for mindwiping him. Instead, Anti-Venom, Sloan, and Octavius were pulled into an unstable portal, resulting in the creation of Atrocity.
- Atrocity (appeared in Spider-Man: Edge of Time, with vocal effects provided by Fred Tatasciore): Atrocity is a result of the violent fusion of Anti-Venom, Doctor Octopus, and Walker Sloan. Spider-Man and Spider-Man 2099 are unaware of its identity, until Spider-Man collects three samples of its DNA and analyzed it to Spider-Man 2099. In the end, Spider-Man defeats Atrocity by luring him into a portal, and Anti-Venom, Sloan, and Octavius' fates are unknown.
- Amazing Spider-Man #399
- The Amazing Spider-Man #673
- Superior Spider-Man #31
- Amazing Spider-Man Extra #3: With Great Responsibility Comes Great Power
- Amazing Spider-Man #603
- Amazing Spider-Man #606
- The Amazing Spider-Man #647
- Marvel Team-Up #4
- Scarlet Spider Vol 2 #14
- Scarlet Spider Vol 2 #17