Locked nucleic acid
A locked nucleic acid (LNA), often referred to as inaccessible RNA, is a modified RNA nucleotide in which the ribose moiety is modified with an extra bridge connecting the 2' oxygen and 4' carbon. The bridge "locks" the ribose in the 3'-endo (North) conformation, which is often found in the A-form duplexes. LNA nucleotides can be mixed with DNA or RNA residues in the oligonucleotide whenever desired and hybridize with DNA or RNA according to Watson-Crick base-pairing rules.
Using LNA based oligonucleotides therapeutically is an emerging field in biotechnology. Allele-specific PCR using LNA allows for the design of shorter primers, without compromising binding specificity.
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