From Wikipedia, the free encyclopedia
Jump to: navigation, search

MeRIPseq [1] stands for methylated RNA immunoprecipitation sequencing, which is a method for detection of post-transcriptional RNA modifications. It is also called m6A-seq.[2]

A variation of the MerIP-seq method was coined by Benjamin Delatte and colleagues in 2016. This variant, called hMerIP-seq (hydroxymethylcytosine RNA immunoprecipitation), uses an antibody that specifically recognizes 5-hydroxymethylcytosine, a modified RNA base affecting in vitro translation and brain development in Drosophila.[3]


  1. ^ Meyer, Kate D.; Saletore, Yogesh; Zumbo, Paul; Elemento, Olivier; Mason, Christopher E.; Jaffrey, Samie R. (31 May 2012). "Comprehensive Analysis of mRNA Methylation Reveals Enrichment in 3′ UTRs and near Stop Codons". Cell. 149 (7): 1635–1646. doi:10.1016/j.cell.2012.05.003. PMC 3383396Freely accessible. PMID 22608085. 
  2. ^ Dominissini, Dan; Moshitch-Moshkovitz, Sharon; Schwartz, Schraga; Salmon-Divon, Mali; Ungar, Lior; Osenberg, Sivan; Cesarkas, Karen; Jacob-Hirsch, Jasmine; Amariglio, Ninette; Kupiec, Martin; Sorek, Rotem; Rechavi, Gideon (28 April 2012). "Topology of the human and mouse m6A RNA methylomes revealed by m6A-seq". Nature. 485 (7397): 201–206. doi:10.1038/nature11112. PMID 22575960. 
  3. ^ Delatte, Benjamin (2016). "Transcriptome-wide distribution and function of RNA hydroxymethylcytosine". Science. 351 (6270): 282–285. doi:10.1126/science.aac5253. PMID 26816380.