Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either "shotgun" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.
- 1 Etymology
- 2 History
- 3 Sequencing
- 4 Bioinformatics
- 5 Data analysis
- 6 Applications
- 7 See also
- 8 References
- 9 External links
The term "metagenomics" was first used by Jo Handelsman, Jon Clardy, Robert M. Goodman, Sean F. Brady, and others, and first appeared in publication in 1998. The term metagenome referenced the idea that a collection of genes sequenced from the environment could be analyzed in a way analogous to the study of a single genome. Recently, Kevin Chen and Lior Pachter (researchers at the University of California, Berkeley) defined metagenomics as "the application of modern genomics technique without the need for isolation and lab cultivation of individual species".
Conventional sequencing begins with a culture of identical cells as a source of DNA. However, early metagenomic studies revealed that there are probably large groups of microorganisms in many environments that cannot be cultured and thus cannot be sequenced. These early studies focused on 16S ribosomal RNA sequences which are relatively short, often conserved within a species, and generally different between species. Many 16S rRNA sequences have been found which do not belong to any known cultured species, indicating that there are numerous non-isolated organisms. These surveys of ribosomal RNA (rRNA) genes taken directly from the environment revealed that cultivation based methods find less than 1% of the bacterial and archaeal species in a sample. Much of the interest in metagenomics comes from these discoveries that showed that the vast majority of microorganisms had previously gone unnoticed.
Early molecular work in the field was conducted by Norman R. Pace and colleagues, who used PCR to explore the diversity of ribosomal RNA sequences. The insights gained from these breakthrough studies led Pace to propose the idea of cloning DNA directly from environmental samples as early as 1985. This led to the first report of isolating and cloning bulk DNA from an environmental sample, published by Pace and colleagues in 1991 while Pace was in the Department of Biology at Indiana University. Considerable efforts ensured that these were not PCR false positives and supported the existence of a complex community of unexplored species. Although this methodology was limited to exploring highly conserved, non-protein coding genes, it did support early microbial morphology-based observations that diversity was far more complex than was known by culturing methods. Soon after that, Healy reported the metagenomic isolation of functional genes from "zoolibraries" constructed from a complex culture of environmental organisms grown in the laboratory on dried grasses in 1995. After leaving the Pace laboratory, Edward DeLong continued in the field and has published work that has largely laid the groundwork for environmental phylogenies based on signature 16S sequences, beginning with his group's construction of libraries from marine samples.
In 2002, Mya Breitbart, Forest Rohwer, and colleagues used environmental shotgun sequencing (see below) to show that 200 liters of seawater contains over 5000 different viruses. Subsequent studies showed that there are more than a thousand viral species in human stool and possibly a million different viruses per kilogram of marine sediment, including many bacteriophages. Essentially all of the viruses in these studies were new species. In 2004, Gene Tyson, Jill Banfield, and colleagues at the University of California, Berkeley and the Joint Genome Institute sequenced DNA extracted from an acid mine drainage system. This effort resulted in the complete, or nearly complete, genomes for a handful of bacteria and archaea that had previously resisted attempts to culture them.
Beginning in 2003, Craig Venter, leader of the privately funded parallel of the Human Genome Project, has led the Global Ocean Sampling Expedition (GOS), circumnavigating the globe and collecting metagenomic samples throughout the journey. All of these samples are sequenced using shotgun sequencing, in hopes that new genomes (and therefore new organisms) would be identified. The pilot project, conducted in the Sargasso Sea, found DNA from nearly 2000 different species, including 148 types of bacteria never before seen. Venter has circumnavigated the globe and thoroughly explored the West Coast of the United States, and completed a two-year expedition to explore the Baltic, Mediterranean and Black Seas. Analysis of the metagenomic data collected during this journey revealed two groups of organisms, one composed of taxa adapted to environmental conditions of 'feast or famine', and a second composed of relatively fewer but more abundantly and widely distributed taxa primarily composed of plankton.
In 2005 Stephan C. Schuster at Penn State University and colleagues published the first sequences of an environmental sample generated with high-throughput sequencing, in this case massively parallel pyrosequencing developed by 454 Life Sciences. Another early paper in this area appeared in 2006 by Robert Edwards, Forest Rohwer, and colleagues at San Diego State University.
Recovery of DNA sequences longer than a few thousand base pairs from environmental samples was very difficult until recent advances in molecular biological techniques allowed the construction of libraries in bacterial artificial chromosomes (BACs), which provided better vectors for molecular cloning.
Advances in bioinformatics, refinements of DNA amplification, and the proliferation of computational power have greatly aided the analysis of DNA sequences recovered from environmental samples, allowing the adaptation of shotgun sequencing to metagenomic samples (known also as whole metagenome shotgun or WMGS sequencing). The approach, used to sequence many cultured microorganisms and the human genome, randomly shears DNA, sequences many short sequences, and reconstructs them into a consensus sequence. Shotgun sequencing reveals genes present in environmental samples. Historically, clone libraries were used to facilitate this sequencing. However, with advances in high throughput sequencing technologies, the cloning step is no longer necessary and greater yields of sequencing data can be obtained without this labour-intensive bottleneck step. Shotgun metagenomics provides information both about which organisms are present and what metabolic processes are possible in the community. Because the collection of DNA from an environment is largely uncontrolled, the most abundant organisms in an environmental sample are most highly represented in the resulting sequence data. To achieve the high coverage needed to fully resolve the genomes of under-represented community members, large samples, often prohibitively so, are needed. On the other hand, the random nature of shotgun sequencing ensures that many of these organisms, which would otherwise go unnoticed using traditional culturing techniques, will be represented by at least some small sequence segments.
The first metagenomic studies conducted using high-throughput sequencing used massively parallel 454 pyrosequencing. Three other technologies commonly applied to environmental sampling are the Ion Torrent Personal Genome Machine, the Illumina MiSeq or HiSeq and the Applied Biosystems SOLiD system. These techniques for sequencing DNA generate shorter fragments than Sanger sequencing; Ion Torrent PGM System and 454 pyrosequencing typically produces ~400 bp reads, Illumina MiSeq produces 400-700bp reads (depending on whether paired end options are used), and SOLiD produce 25-75 bp reads. Historically, these read lengths were significantly shorter than the typical Sanger sequencing read length of ~750 bp, however the Illumina technology is quickly coming close to this benchmark. However, this limitation is compensated for by the much larger number of sequence reads. In 2009, pyrosequenced metagenomes generate 200–500 megabases, and Illumina platforms generate around 20–50 gigabases, but these outputs have increased by orders of magnitude in recent years. An additional advantage to high throughput sequencing is that this technique does not require cloning the DNA before sequencing, removing one of the main biases and bottlenecks in environmental sampling.
The data generated by metagenomics experiments are both enormous and inherently noisy, containing fragmented data representing as many as 10,000 species. The sequencing of the cow rumen metagenome generated 279 gigabases, or 279 billion base pairs of nucleotide sequence data, while the human gut microbiome gene catalog identified 3.3 million genes assembled from 567.7 gigabases of sequence data. Collecting, curating, and extracting useful biological information from datasets of this size represent significant computational challenges for researchers.
The first step of metagenomic data analysis requires the execution of certain pre-filtering steps, including the removal of redundant, low-quality sequences and sequences of probable eukaryotic origin (especially in metagenomes of human origin). The methods available for the removal of contaminating eukaryotic genomic DNA sequences include Eu-Detect and DeConseq.
DNA sequence data from genomic and metagenomic projects are essentially the same, but genomic sequence data offers higher coverage while metagenomic data is usually highly non-redundant. Furthermore, the increased use of second-generation sequencing technologies with short read lengths means that much of future metagenomic data will be error-prone. Taken in combination, these factors make the assembly of metagenomic sequence reads into genomes difficult and unreliable. Misassemblies are caused by the presence of repetitive DNA sequences that make assembly especially difficult because of the difference in the relative abundance of species present in the sample. Misassemblies can also involve the combination of sequences from more than one species into chimeric contigs.
There are several assembly programs, most of which can use information from paired-end tags in order to improve the accuracy of assemblies. Some programs, such as Phrap or Celera Assembler, were designed to be used to assemble single genomes but nevertheless produce good results when assembling metagenomic data sets. Other programs, such as Velvet assembler, have been optimized for the shorter reads produced by second-generation sequencing through the use of de Bruijn graphs. The use of reference genomes allows researchers to improve the assembly of the most abundant microbial species, but this approach is limited by the small subset of microbial phyla for which sequenced genomes are available. After an assembly is created, an additional challenge is "metagenomic deconvolution", or determining which sequences come from which species in the sample.
Metagenomic analysis pipelines use two approaches in the annotation of coding regions in the assembled contigs. The first approach is to identify genes based upon homology with genes that are already publicly available in sequence databases, usually by simple BLAST searches. This type of approach is implemented in the program MEGAN4.  The second, ab initio, uses intrinsic features of the sequence to predict coding regions based upon gene training sets from related organisms. This is the approach taken by programs such as GeneMark and GLIMMER. The main advantage of ab initio prediction is that it enables the detection of coding regions that lack homologs in the sequence databases; however, it is most accurate when there are large regions of contiguous genomic DNA available for comparison.
Gene annotations provide the "what", while measurements of species diversity provide the "who". In order to connect community composition and function in metagenomes, sequences must be binned. Binning is the process of associating a particular sequence with an organism. In similarity-based binning, methods such as BLAST are used to rapidly search for phylogenetic markers or otherwise similar sequences in existing public databases. This approach is implemented in MEGAN. Another tool, PhymmBL, uses interpolated Markov models to assign reads. MetaPhlAn and AMPHORA are methods based on unique clade-specific markers for estimating organismal relative abundances with improved computational performances. Recent methods, such as SLIMM, use read coverage landscape of individual reference genomes to minimize false-positive hits and get reliable relative abundances. In composition based binning, methods use intrinsic features of the sequence, such as oligonucleotide frequencies or codon usage bias. Once sequences are binned, it is possible to carry out comparative analysis of diversity and richness.
The massive amount of exponentially growing sequence data is a daunting challenge that is complicated by the complexity of the metadata associated with metagenomic projects. Metadata includes detailed information about the three-dimensional (including depth, or height) geography and environmental features of the sample, physical data about the sample site, and the methodology of the sampling. This information is necessary both to ensure replicability and to enable downstream analysis. Because of its importance, metadata and collaborative data review and curation require standardized data formats located in specialized databases, such as the Genomes OnLine Database (GOLD).
Several tools have been developed to integrate metadata and sequence data, allowing downstream comparative analyses of different datasets using a number of ecological indices. In 2007, Folker Meyer and Robert Edwards and a team at Argonne National Laboratory and the University of Chicago released the Metagenomics Rapid Annotation using Subsystem Technology server (MG-RAST) a community resource for metagenome data set analysis. As of June 2012 over 14.8 terabases (14x1012 bases) of DNA have been analyzed, with more than 10,000 public data sets freely available for comparison within MG-RAST. Over 8,000 users now have submitted a total of 50,000 metagenomes to MG-RAST. The Integrated Microbial Genomes/Metagenomes (IMG/M) system also provides a collection of tools for functional analysis of microbial communities based on their metagenome sequence, based upon reference isolate genomes included from the Integrated Microbial Genomes (IMG) system and the Genomic Encyclopedia of Bacteria and Archaea (GEBA) project.
One of the first standalone tools for analysing high-throughput metagenome shotgun data was MEGAN (MEta Genome ANalyzer). A first version of the program was used in 2005 to analyse the metagenomic context of DNA sequences obtained from a mammoth bone. Based on a BLAST comparison against a reference database, this tool performs both taxonomic and functional binning, by placing the reads onto the nodes of the NCBI taxonomy using a simple lowest common ancestor (LCA) algorithm or onto the nodes of the SEED or KEGG classifications, respectively.
With the advent of fast and inexpensive sequencing instruments, the growth of databases of DNA sequences is now exponential (e.g., the NCBI GenBank database ). Faster and efficient tools are needed to keep pace with the high-throughput sequencing, because the BLAST-based approaches such as MG-RAST or MEGAN run slowly to annotate large samples (e.g., several hours to process a small/medium size dataset/sample ). Thus, ultra-fast classifiers have recently emerged, thanks to more affordable powerful servers. These tools can perform the taxonomic annotation at extremely high speed, for example CLARK  (according to CLARK's authors, it can classify accurately "32 million metagenomic short reads per minute"). At such a speed, a very large dataset/sample of a billion short reads can be processed in about 30 minutes.
Comparative analyses between metagenomes can provide additional insight into the function of complex microbial communities and their role in host health. Pairwise or multiple comparisons between metagenomes can be made at the level of sequence composition (comparing GC-content or genome size), taxonomic diversity, or functional complement. Comparisons of population structure and phylogenetic diversity can be made on the basis of 16S and other phylogenetic marker genes, or—in the case of low-diversity communities—by genome reconstruction from the metagenomic dataset. Functional comparisons between metagenomes may be made by comparing sequences against reference databases such as COG or KEGG, and tabulating the abundance by category and evaluating any differences for statistical significance. This gene-centric approach emphasizes the functional complement of the community as a whole rather than taxonomic groups, and shows that the functional complements are analogous under similar environmental conditions. Consequently, metadata on the environmental context of the metagenomic sample is especially important in comparative analyses, as it provides researchers with the ability to study the effect of habitat upon community structure and function.
Additionally, several studies have also utilized oligonucleotide usage patterns to identify the differences across diverse microbial communities. Examples of such methodologies include the dinucleotide relative abundance approach by Willner et al. and the HabiSign approach of Ghosh et al. This latter study also indicated that differences in tetranucleotide usage patterns can be used to identify genes (or metagenomic reads) originating from specific habitats. Additionally some methods as TriageTools or Compareads detect similar reads between two read sets. The similarity measure they apply on reads is based on a number of identical words of length k shared by pairs of reads.
A key goal in comparative metagenomics is to identify microbial group(s) which are responsible for conferring specific characteristics to a given environment. However, due to issues in the sequencing technologies artifacts need to be accounted for like in metagenomeSeq. Others have characterized inter-microbial interactions between the resident microbial groups. A GUI-based comparative metagenomic analysis application called Community-Analyzer has been developed by Kuntal et al.  which implements a correlation-based graph layout algorithm that not only facilitates a quick visualization of the differences in the analyzed microbial communities (in terms of their taxonomic composition), but also provides insights into the inherent inter-microbial interactions occurring therein. Notably, this layout algorithm also enables grouping of the metagenomes based on the probable inter-microbial interaction patterns rather than simply comparing abundance values of various taxonomic groups. In addition, the tool implements several interactive GUI-based functionalities that enable users to perform standard comparative analyses across microbiomes.
In many bacterial communities, natural or engineered (such as bioreactors), there is significant division of labor in metabolism (Syntrophy), during which the waste products of some organisms are metabolites for others. In one such system, the methanogenic bioreactor, functional stability requires the presence of several syntrophic species (Syntrophobacterales and Synergistia) working together in order to turn raw resources into fully metabolized waste (methane). Using comparative gene studies and expression experiments with microarrays or proteomics researchers can piece together a metabolic network that goes beyond species boundaries. Such studies require detailed knowledge about which versions of which proteins are coded by which species and even by which strains of which species. Therefore, community genomic information is another fundamental tool (with metabolomics and proteomics) in the quest to determine how metabolites are transferred and transformed by a community.
Metagenomics allows researchers to access the functional and metabolic diversity of microbial communities, but it cannot show which of these processes are active. The extraction and analysis of metagenomic mRNA (the metatranscriptome) provides information on the regulation and expression profiles of complex communities. Because of the technical difficulties (the short half-life of mRNA, for example) in the collection of environmental RNA there have been relatively few in situ metatranscriptomic studies of microbial communities to date. While originally limited to microarray technology, metatranscriptomcs studies have made use of direct high-throughput cDNA sequencing to provide whole-genome expression and quantification of a microbial community, as first employed by Leininger et al. (2006) in their analysis of ammonia oxidation in soils.
Metagenomic sequencing is particularly useful in the study of viral communities. As viruses lack a shared universal phylogenetic marker (as 16S RNA for bacteria and archaea, and 18S RNA for eukarya), the only way to access the genetic diversity of the viral community from an environmental sample is through metagenomics. Viral metagenomes (also called viromes) should thus provide more and more information about viral diversity and evolution. For example, a metagenomic pipeline called Giant Virus Finder showed the first evidence of existence of giant viruses in a saline desert  and in Antarctic dry valleys .
Metagenomics has the potential to advance knowledge in a wide variety of fields. It can also be applied to solve practical challenges in medicine, engineering, agriculture, sustainability and ecology.
Infectious Disease Diagnosis
Differentiating between infectious and non-infectious illness, and identifying the underlying etiology of infection, can be quite challenging. For example, more than half of cases of encephalitis remain undiagnosed, despite extensive testing using state-of-the-art clinical laboratory methods. Metagenomic sequencing shows promise as a sensitive and rapid method to diagnose infection by comparing genetic material found in a patient's sample to a database of thousands of bacteria, viruses, and other pathogens.
Gut Microbe Characterization
Microbial communities play a key role in preserving human health, but their composition and the mechanism by which they do so remains mysterious. Metagenomic sequencing is being used to characterize the microbial communities from 15-18 body sites from at least 250 individuals. This is part of the Human Microbiome initiative with primary goals to determine if there is a core human microbiome, to understand the changes in the human microbiome that can be correlated with human health, and to develop new technological and bioinformatics tools to support these goals.
Another medical study as part of the MetaHit (Metagenomics of the Human Intestinal Tract) project consisted of 124 individuals from Denmark and Spain consisting of healthy, overweight, and irritable bowel disease patients. The study attempted to categorize the depth and phylogenetic diversity of gastrointestinal bacteria. Using Illumina GA sequence data and SOAPdenovo, a de Bruijn graph-based tool specifically designed for assembly short reads, they were able to generate 6.58 million contigs greater than 500 bp for a total contig length of 10.3 Gb and a N50 length of 2.2 kb.
The study demonstrated that two bacterial divisions, Bacteroidetes and Firmicutes, constitute over 90% of the known phylogenetic categories that dominate distal gut bacteria. Using the relative gene frequencies found within the gut these researchers identified 1,244 metagenomic clusters that are critically important for the health of the intestinal tract. There are two types of functions in these range clusters: housekeeping and those specific to the intestine. The housekeeping gene clusters are required in all bacteria and are often major players in the main metabolic pathways including central carbon metabolism and amino acid synthesis. The gut-specific functions include adhesion to host proteins and the harvesting of sugars from globoseries glycolipids. Patients with irritable bowel syndrome were shown to exhibit 25% fewer genes and lower bacterial diversity than individuals not suffering from irritable bowel syndrome indicating that changes in patients’ gut biome diversity may be associated with this condition.
While these studies highlight some potentially valuable medical applications, only 31-48.8% of the reads could be aligned to 194 public human gut bacterial genomes and 7.6-21.2% to bacterial genomes available in GenBank which indicates that there is still far more research necessary to capture novel bacterial genomes.
Biofuels are fuels derived from biomass conversion, as in the conversion of cellulose contained in corn stalks, switchgrass, and other biomass into cellulosic ethanol. This process is dependent upon microbial consortia that transform the cellulose into sugars, followed by the fermentation of the sugars into ethanol. Microbes also produce a variety of sources of bioenergy including methane and hydrogen.
The efficient industrial-scale deconstruction of biomass requires novel enzymes with higher productivity and lower cost. Metagenomic approaches to the analysis of complex microbial communities allow the targeted screening of enzymes with industrial applications in biofuel production, such as glycoside hydrolases. Furthermore, knowledge of how these microbial communities function is required to control them, and metagenomics is a key tool in their understanding. Metagenomic approaches allow comparative analyses between convergent microbial systems like biogas fermenters or insect herbivores such as the fungus garden of the leafcutter ants.
Metagenomics can improve strategies for monitoring the impact of pollutants on ecosystems and for cleaning up contaminated environments. Increased understanding of how microbial communities cope with pollutants improves assessments of the potential of contaminated sites to recover from pollution and increases the chances of bioaugmentation or biostimulation trials to succeed.
Microbial communities produce a vast array of biologically active chemicals that are used in competition and communication. Many of the drugs in use today were originally uncovered in microbes; recent progress in mining the rich genetic resource of non-culturable microbes has led to the discovery of new genes, enzymes, and natural products. The application of metagenomics has allowed the development of commodity and fine chemicals, agrochemicals and pharmaceuticals where the benefit of enzyme-catalyzed chiral synthesis is increasingly recognized.
Two types of analysis are used in the bioprospecting of metagenomic data: function-driven screening for an expressed trait, and sequence-driven screening for DNA sequences of interest. Function-driven analysis seeks to identify clones expressing a desired trait or useful activity, followed by biochemical characterization and sequence analysis. This approach is limited by availability of a suitable screen and the requirement that the desired trait be expressed in the host cell. Moreover, the low rate of discovery (less than one per 1,000 clones screened) and its labor-intensive nature further limit this approach. In contrast, sequence-driven analysis uses conserved DNA sequences to design PCR primers to screen clones for the sequence of interest. In comparison to cloning-based approaches, using a sequence-only approach further reduces the amount of bench work required. The application of massively parallel sequencing also greatly increases the amount of sequence data generated, which require high-throughput bioinformatic analysis pipelines. The sequence-driven approach to screening is limited by the breadth and accuracy of gene functions present in public sequence databases. In practice, experiments make use of a combination of both functional and sequence-based approaches based upon the function of interest, the complexity of the sample to be screened, and other factors.
The soils in which plants grow are inhabited by microbial communities, with one gram of soil containing around 109-1010 microbial cells which comprise about one gigabase of sequence information. The microbial communities which inhabit soils are some of the most complex known to science, and remain poorly understood despite their economic importance. Microbial consortia perform a wide variety of ecosystem services necessary for plant growth, including fixing atmospheric nitrogen, nutrient cycling, disease suppression, and sequester iron and other metals. Functional metagenomics strategies are being used to explore the interactions between plants and microbes through cultivation-independent study of these microbial communities. By allowing insights into the role of previously uncultivated or rare community members in nutrient cycling and the promotion of plant growth, metagenomic approaches can contribute to improved disease detection in crops and livestock and the adaptation of enhanced farming practices which improve crop health by harnessing the relationship between microbes and plants.
Metagenomics can provide valuable insights into the functional ecology of environmental communities. Metagenomic analysis of the bacterial consortia found in the defecations of Australian sea lions suggests that nutrient-rich sea lion faeces may be an important nutrient source for coastal ecosystems. This is because the bacteria that are expelled simultaneously with the defecations are adept at breaking down the nutrients in the faeces into a bioavailable form that can be taken up into the food chain.
DNA sequencing can also be used more broadly to identify species present in a body of water, debris filtered from the air, or sample of dirt. This can establish the range of invasive species and endangered species, and track seasonal populations.
- Hugenholz, P; Goebel BM; Pace NR (1 September 1998). "Impact of Culture-Independent Studies on the Emerging Phylogenetic View of Bacterial Diversity". J. Bacteriol. 180 (18): 4765–74. PMC . PMID 9733676.
- Eisen, JA (2007). "Environmental Shotgun Sequencing: Its Potential and Challenges for Studying the Hidden World of Microbes". PLoS Biology. 5 (3): e82. doi:10.1371/journal.pbio.0050082. PMC . PMID 17355177.
- Marco, D, ed. (2011). Metagenomics: Current Innovations and Future Trends. Caister Academic Press. ISBN 978-1-904455-87-5.
- Handelsman, J.; Rondon, M. R.; Brady, S. F.; Clardy, J.; Goodman, R. M. (1998). "Molecular biological access to the chemistry of unknown soil microbes: A new frontier for natural products". Chemistry & Biology. 5 (10): R245–R249. doi:10.1016/S1074-5521(98)90108-9. PMID 9818143..
- Chen, K.; Pachter, L. (2005). "Bioinformatics for Whole-Genome Shotgun Sequencing of Microbial Communities". PLoS Computational Biology. 1 (2): e24. doi:10.1371/journal.pcbi.0010024. PMC . PMID 16110337.
- Lane, DJ; Pace B; Olsen GJ; Stahl DA; Sogin ML; Pace NR (1985). "Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses". Proceedings of the National Academy of Sciences. 82 (20): 6955–9. Bibcode:1985PNAS...82.6955L. doi:10.1073/pnas.82.20.6955. PMC . PMID 2413450.
- Pace, NR; DA Stahl; DJ Lane; GJ Olsen (1985). "Analyzing natural microbial populations by rRNA sequences". ASM News. 51: 4–12.
- Pace, NR; Delong, EF; Pace, NR (1991). "Analysis of a marine picoplankton community by 16S rRNA gene cloning and sequencing". Journal of Bacteriology. 173 (14): 4371–4378. PMC . PMID 2066334.
- Healy, FG; RM Ray; HC Aldrich; AC Wilkie; LO Ingram; KT Shanmugam (1995). "Direct isolation of functional genes encoding cellulases from the microbial consortia in a thermophilic, anaerobic digester maintained on lignocellulose". Appl. Microbiol Biotechnol. 43 (4): 667–74. doi:10.1007/BF00164771. PMID 7546604.
- Stein, JL; TL Marsh; KY Wu; H Shizuya; EF DeLong (1996). "Characterization of uncultivated prokaryotes: isolation and analysis of a 40-kilobase-pair genome fragment from a planktonic marine archaeon". Journal of Bacteriology. 178 (3): 591–599. PMC . PMID 8550487.
- Breitbart, M; Salamon P; Andresen B; Mahaffy JM; Segall AM; Mead D; Azam F; Rohwer F (2002). "Genomic analysis of uncultured marine viral communities". Proceedings of the National Academy of Sciences of the United States of America. 99 (22): 14250–14255. Bibcode:2002PNAS...9914250B. doi:10.1073/pnas.202488399. PMC . PMID 12384570.
- Tyson, GW; Chapman J; Hugenholtz P; Allen EE; Ram RJ; Richardson PM; Solovyev VV; Rubin EM; Rokhsar DS; Banfield JF (2004). "Insights into community structure and metabolism by reconstruction of microbial genomes from the environment". Nature. 428 (6978): 37–43. Bibcode:2004Natur.428...37T. doi:10.1038/nature02340. PMID 14961025.(subscription required)
- Hugenholz, P (2002). "Exploring prokaryotic diversity in the genomic era". Genome Biology. 3 (2): 1–8. doi:10.1186/gb-2002-3-2-reviews0003. PMC . PMID 11864374.
- Thomas, T.; Gilbert, J.; Meyer, F. (2012). "Metagenomics - a guide from sampling to data analysis". Microbial Informatics and Experimentation. 2 (1): 3. doi:10.1186/2042-5783-2-3. PMC . PMID 22587947.
- Venter, JC; Remington K; Heidelberg JF; Halpern AL; Rusch D; Eisen JA; Wu D; Paulsen I; Nelson KE; Nelson W; Fouts DE; Levy S; Knap AH; Lomas MW; Nealson K; White O; Peterson J; Hoffman J; Parsons R; Baden-Tillson H; Pfannkoch C; Rogers Y; Smith HO (2004). "Environmental Genome Shotgun Sequencing of the Sargasso Sea". Science. 304 (5667): 66–74. Bibcode:2004Sci...304...66V. doi:10.1126/science.1093857. PMID 15001713.
- Yooseph, Shibu; Kenneth H. Nealson; Douglas B. Rusch; John P. McCrow; Christopher L. Dupont; Maria Kim; Justin Johnson; Robert Montgomery; Steve Ferriera; Karen Beeson; Shannon J. Williamson; Andrey Tovchigrechko; Andrew E. Allen; Lisa A. Zeigler; Granger Sutton; Eric Eisenstadt; Yu-Hui Rogers; Robert Friedman; Marvin Frazier; J. Craig Venter (4 November 2010). "Genomic and functional adaptation in surface ocean planktonic prokaryotes". Nature. 468 (7320): 60–66. Bibcode:2010Natur.468...60Y. doi:10.1038/nature09530. ISSN 0028-0836. PMID 21048761.(subscription required)
- Poinar, HN; Schwarz, C; Qi, J; Shapiro, B; Macphee, RD; Buigues, B; Tikhonov, A; Huson, D; Tomsho, LP; Auch, A; Rampp, M; Miller, W; Schuster, SC (2006). "Metagenomics to Paleogenomics: Large-Scale Sequencing of Mammoth DNA". Science. 311 (5759): 392–394. Bibcode:2006Sci...311..392P. doi:10.1126/science.1123360. PMID 16368896.
- Edwards, RA; Rodriguez-Brito B; Wegley L; Haynes M; Breitbart M; Peterson DM; Saar MO; Alexander S; Alexander EC; Rohwer F (2006). "Using pyrosequencing to shed light on deep mine microbial ecology". BMC Genomics. 7: 57. doi:10.1186/1471-2164-7-57. PMC . PMID 16549033.
- Beja, O.; Suzuki, MT; Koonin, EV; Aravind, L; Hadd, A; Nguyen, LP; Villacorta, R; Amjadi, M; Garrigues, C (2000). "Construction and analysis of bacterial artificial chromosome libraries from a marine microbial assemblage". Environmental Microbiology. 2 (5): 516–29. doi:10.1046/j.1462-2920.2000.00133.x. PMID 11233160.
- Nicola, Segata; Daniela Boernigen; Timothy L Tickle; Xochitl C Morgan; Wendy S Garrett; Curtis Huttenhower (2013). "Computational meta'omics for microbial community studies". Molecular Systems Biology. 9 (666): 666. doi:10.1038/msb.2013.22. PMC . PMID 23670539.
- Rodrigue, S. B.; Materna, A. C.; Timberlake, S. C.; Blackburn, M. C.; Malmstrom, R. R.; Alm, E. J.; Chisholm, S. W. (2010). Gilbert, Jack Anthony, ed. "Unlocking Short Read Sequencing for Metagenomics". PLoS ONE. 5 (7): e11840. doi:10.1371/journal.pone.0011840. PMC . PMID 20676378.
- Schuster, S. C. (2007). "Next-generation sequencing transforms today's biology". Nature Methods. 5 (1): 16–18. doi:10.1038/nmeth1156. PMID 18165802.
- "Metagenomics versus Moore's law". Nature Methods. 6 (9): 623. 2009. doi:10.1038/nmeth0909-623.
- Wooley, J. C.; Godzik, A.; Friedberg, I. (2010). Bourne, Philip E., ed. "A Primer on Metagenomics". PLoS Computational Biology. 6 (2): e1000667. doi:10.1371/journal.pcbi.1000667. PMC . PMID 20195499.
- Hess, Matthias; Alexander Sczyrba; Rob Egan; Tae-Wan Kim; Harshal Chokhawala; Gary Schroth; Shujun Luo; Douglas S Clark; Feng Chen; Tao Zhang; Roderick I Mackie; Len A Pennacchio; Susannah G Tringe; Axel Visel; Tanja Woyke; Zhong Wang; Edward M Rubin (28 January 2011). "Metagenomic discovery of biomass-degrading genes and genomes from cow rumen". Science. 331 (6016): 463–467. Bibcode:2011Sci...331..463H. doi:10.1126/science.1200387. ISSN 1095-9203. PMID 21273488.
- Qin, Junjie; Ruiqiang Li; Jeroen Raes; Manimozhiyan Arumugam; Kristoffer Solvsten Burgdorf; Chaysavanh Manichanh; Trine Nielsen; Nicolas Pons; Florence Levenez; Takuji Yamada; Daniel R. Mende; Junhua Li; Junming Xu; Shaochuan Li; Dongfang Li; Jianjun Cao; Bo Wang; Huiqing Liang; Huisong Zheng; Yinlong Xie; Julien Tap; Patricia Lepage; Marcelo Bertalan; Jean-Michel Batto; Torben Hansen; Denis Le Paslier; Allan Linneberg; H. Bjorn Nielsen; Eric Pelletier; Pierre Renault; Thomas Sicheritz-Ponten; Keith Turner; Hongmei Zhu; Chang Yu; Shengting Li; Min Jian; Yan Zhou; Yingrui Li; Xiuqing Zhang; Songgang Li; Nan Qin; Huanming Yang; Jian Wang; Soren Brunak; Joel Dore; Francisco Guarner; Karsten Kristiansen; Oluf Pedersen; Julian Parkhill; Jean Weissenbach; Peer Bork; S. Dusko Ehrlich; Jun Wang (4 March 2010). "A human gut microbial gene catalogue established by metagenomic sequencing". Nature. 464 (7285): 59–65. Bibcode:2010Natur.464...59.. doi:10.1038/nature08821. ISSN 0028-0836. PMC . PMID 20203603.(subscription required)
- Paulson, Joseph; O Colin Stine; Hector Corrada Bravo; Mihai Pop (2013). "Differential abundance analysis for microbial marker-gene surveys". Nature Methods. 10 (12): 1200–1202. doi:10.1038/nmeth.2658. PMC . PMID 24076764.
- Committee on Metagenomics: Challenges and Functional Applications, National Research Council (2007). The New Science of Metagenomics: Revealing the Secrets of Our Microbial Planet. Washington, D.C.: The National Academies Press. ISBN 0-309-10676-1.
- Mende, Daniel R.; Alison S. Waller; Shinichi Sunagawa; Aino I. Järvelin; Michelle M. Chan; Manimozhiyan Arumugam; Jeroen Raes; Peer Bork (2012-02-23). "Assessment of Metagenomic Assembly Using Simulated Next Generation Sequencing Data". PLoS ONE. 7 (2): e31386. Bibcode:2012PLoSO...731386M. doi:10.1371/journal.pone.0031386. ISSN 1932-6203. PMC . PMID 22384016.
- Balzer, S.; Malde, K.; Grohme, M. A.; Jonassen, I. (2013). "Filtering duplicate reads from 454 pyrosequencing data". Bioinformatics. 29 (7): 830–836. doi:10.1093/bioinformatics/btt047. PMC . PMID 23376350.
- Mohammed, MH; Sudha Chadaram; Dinakar Komanduri; Tarini Shankar Ghosh; Sharmila S Mande (2011). "Eu-Detect: an algorithm for detecting eukaryotic sequences in metagenomic data sets". Journal of Biosciences. 36 (4): 709–717. doi:10.1007/s12038-011-9105-2. PMID 21857117.
- R, Schmeider; R Edwards (2011). "Fast identification and removal of sequence contamination from genomic and metagenomic datasets". PLoS ONE. 6 (3): e17288. Bibcode:2011PLoSO...617288S. doi:10.1371/journal.pone.0017288. PMC . PMID 21408061.
- Kunin, V.; Copeland, A.; Lapidus, A.; Mavromatis, K.; Hugenholtz, P. (2008). "A Bioinformatician's Guide to Metagenomics". Microbiology and Molecular Biology Reviews. 72 (4): 557–578, Table 578 Contents. doi:10.1128/MMBR.00009-08. PMC . PMID 19052320.
- Burton, J. N.; Liachko, I.; Dunham, M. J.; Shendure, J. (2014). "Species-Level Deconvolution of Metagenome Assemblies with Hi-C-Based Contact Probability Maps". G3: Genes, Genomes, Genetics. 4: 1339–1346. doi:10.1534/g3.114.011825.
- Huson, Daniel H; S. Mitra; N. Weber; H. Ruscheweyh; Stephan C. Schuster (June 2011). "Integrative analysis of environmental sequences using MEGAN4". Genome Research. 21 (9): 1552–1560. doi:10.1101/gr.120618.111. PMC . PMID 21690186.
- Zhu, Wenhan; Lomsadze Alex; Borodovsky Mark (2010). "Ab initio gene identification in metagenomic sequences". Nucleic Acids Research. 38 (12): e132. doi:10.1093/nar/gkq275. PMC . PMID 20403810.
- Hug, Laura A.; Baker, Brett J.; Anantharaman, Karthik; Brown, Christopher T.; Probst, Alexander J.; Castelle, Cindy J.; Butterfield, Cristina N.; Hernsdorf, Alex W.; Amano, Yuki; Ise, Kotaro; Suzuki, Yohey; Dudek, Natasha; Relman, David A.; Finstad, Kari M.; Amundson, Ronald; Thomas, Brian C.; Banfield, Jillian F. (11 April 2016). "A new view of the tree of life". Nature Microbiology. 1: 16048. doi:10.1038/nmicrobiol.2016.48. PMID 27572647.
- Konopka, A. (2009). "What is microbial community ecology?". The ISME Journal. 3 (11): 1223–1230. doi:10.1038/ismej.2009.88. PMID 19657372.
- Huson, Daniel H; A. Auch; Ji Qi; Stephan C Schuster (January 2007). "MEGAN Analysis of Metagenomic Data". Genome Research. 17 (3): 377–386. doi:10.1101/gr.5969107. PMC . PMID 17255551.
- Nicola, Segata; Levi Waldron; Annalisa Ballarini; Vagheesh Narasimhan; Olivier Jousson; Curtis Huttenhower (2012). "Metagenomic microbial community profiling using unique clade-specific marker genes". Nature Methods. 9 (8): 811–814. doi:10.1038/nmeth.2066. PMC . PMID 22688413.
- Dadi, Temesgen Hailemariam; Renard, Bernhard Y.; Wieler, Lothar H.; Semmler, Torsten; Reinert, Knut (2017). "SLIMM: species level identification of microorganisms from metagenomes". PeerJ. 5: e3138. doi:10.7717/peerj.3138. ISSN 2167-8359.
- Pagani, Ioanna; Konstantinos Liolios; Jakob Jansson; I-Min A Chen; Tatyana Smirnova; Bahador Nosrat; Victor M Markowitz; Nikos C Kyrpides (1 December 2011). "The Genomes OnLine Database (GOLD) v.4: status of genomic and metagenomic projects and their associated metadata". Nucleic Acids Research. 40 (1): D571–9. doi:10.1093/nar/gkr1100. ISSN 1362-4962. PMC . PMID 22135293.
- Meyer, F; Paarmann D; D'Souza M; Olson R; Glass EM; Kubal M; Paczian T; Rodriguez A; Stevens R; Wilke A; Wilkening J; Edwards RA (2008). "The metagenomics RAST server – a public resource for the automatic phylogenetic and functional analysis of metagenomes". BMC Bioinformatics. 9: 0. doi:10.1186/1471-2105-9-386. PMC . PMID 18803844.
- Markowitz, V. M.; Chen, I. -M. A.; Chu, K.; Szeto, E.; Palaniappan, K.; Grechkin, Y.; Ratner, A.; Jacob, B.; Pati, A.; Huntemann, M.; Liolios, K.; Pagani, I.; Anderson, I.; Mavromatis, K.; Ivanova, N. N.; Kyrpides, N. C. (2011). "IMG/M: The integrated metagenome data management and comparative analysis system". Nucleic Acids Research. 40 (Database issue): D123–D129. doi:10.1093/nar/gkr975. PMC . PMID 22086953.
- Mitra, Suparna; Paul Rupek; Daniel C Richter; Tim Urich; Jack A Gilbert; Folker Meyer; Andreas Wilke; Daniel H Huson (2011). "Functional analysis of metagenomes and metatranscriptomes using SEED and KEGG". BMC Bioinformatics. 12 Suppl 1: S21. doi:10.1186/1471-2105-12-S1-S21. ISSN 1471-2105. PMC . PMID 21342551.
- Benson, Dennis; Mark Cavanaugh; Karen Clark; et al. (2013). "Genbank". Nucleic Acids Research. 41: D36–D42. doi:10.1093/nar/gks1195. PMC . PMID 23193287.
- Bazinet, Adam; Michael Cummings (2012). "A comparative evaluation of sequence classification programs". BMC Bioinformatics. 13.
- Ounit, Rachid; Steve Wanamaker; Timothy Close; Stefano Lonardi (2015). "CLARK: fast and accurate classification of metagenomic and genomic sequences using discriminative k-mers". BMC Genomics. 16. doi:10.1186/s12864-015-1419-2. PMC . PMID 25879410.
- Kurokawa, Ken; Takehiko Itoh; Tomomi Kuwahara; Kenshiro Oshima; Hidehiro Toh; Atsushi Toyoda; Hideto Takami; Hidetoshi Morita; Vineet K. Sharma; Tulika P. Srivastava; Todd D. Taylor; Hideki Noguchi; Hiroshi Mori; Yoshitoshi Ogura; Dusko S. Ehrlich; Kikuji Itoh; Toshihisa Takagi; Yoshiyuki Sakaki; Tetsuya Hayashi; Masahira Hattori (1 January 2007). "Comparative Metagenomics Revealed Commonly Enriched Gene Sets in Human Gut Microbiomes". DNA Research. 14 (4): 169–181. doi:10.1093/dnares/dsm018. PMC . PMID 17916580. Retrieved 18 December 2011.
- Simon, C.; Daniel, R. (2010). "Metagenomic Analyses: Past and Future Trends". Applied and Environmental Microbiology. 77 (4): 1153–1161. doi:10.1128/AEM.02345-10. PMC . PMID 21169428.
- Willner, D; RV Thurber; F Rohwer (2009). "Metagenomic signatures of 86 microbial and viral metagenomes.". Environmental Microbiology. 11 (7): 1752–66. doi:10.1111/j.1462-2920.2009.01901.x. PMID 19302541.
- Ghosh, Tarini Shankar; Monzoorul Haque Mohammed; Hannah Rajasingh; Sudha Chadaram; Sharmila S Mande (2011). "HabiSign: a novel approach for comparison of metagenomes and rapid identification of habitat-specific sequences.". BMC Bioinformatics. 12 (Supplement 13): S9. doi:10.1186/1471-2105-12-s13-s9. PMC . PMID 22373355.
- Fimereli, D.; Detours, V.; Konopka, T. (13 February 2013). "TriageTools: tools for partitioning and prioritizing analysis of high-throughput sequencing data". Nucleic Acids Research. 41 (7): e86–e86. doi:10.1093/nar/gkt094. PMC . PMID 23408855.
- Maillet, Nicolas; Lemaitre, Claire; Chikhi, Rayan; Lavenier, Dominique; Peterlongo, Pierre. "Compareads: comparing huge metagenomic experiments". BMC Bioinformatics. 13 (Suppl 19): S10. doi:10.1186/1471-2105-13-S19-S10. PMC . PMID 23282463.
- Bhusan, Kuntal Kumar; Tarini Shankar Ghosh; Sharmila S Mande (2013). "Community-analyzer: a platform for visualizing and comparing microbial community structure across microbiomes.". Genomics. 102: 409–418. doi:10.1016/j.ygeno.2013.08.004. PMID 23978768.
- Werner, Jeffrey J.; Dan Knights; Marcelo L. Garcia; Nicholas B. Scalfone; Samual Smith; Kevin Yarasheski; Theresa A. Cummings; Allen R. Beers; Rob Knight; Largus T. Angenent (8 March 2011). "Bacterial community structures are unique and resilient in full-scale bioenergy systems". Proceedings of the National Academy of Sciences of the United States of America. 108 (10): 4158–4163. Bibcode:2011PNAS..108.4158W. doi:10.1073/pnas.1015676108. ISSN 0027-8424. PMC . PMID 21368115.
- McInerney, Michael J.; Jessica R. Sieber; Robert P. Gunsalus (December 2009). "Syntrophy in Anaerobic Global Carbon Cycles". Current opinion in biotechnology. 20 (6): 623–632. doi:10.1016/j.copbio.2009.10.001. ISSN 0958-1669. PMC . PMID 19897353.
- Klitgord, N.; Segrè, D. (2011). "Ecosystems biology of microbial metabolism". Current Opinion in Biotechnology. 22 (4): 541–546. doi:10.1016/j.copbio.2011.04.018. PMID 21592777.
- Leininger, S.; Urich, T.; Schloter, M.; Schwark, L.; Qi, J.; Nicol, G. W.; Prosser, J. I.; Schuster, S. C.; Schleper, C. (2006). "Archaea predominate among ammonia-oxidizing prokaryotes in soils". Nature. 442 (7104): 806–809. doi:10.1038/nature04983. PMID 16915287.
- Kristensen, DM; Mushegian AR; Dolja VV; Koonin EV (2009). "New dimensions of the virus world discovered through metagenomics". Trends in Microbiology. 18 (1): 11–19. doi:10.1016/j.tim.2009.11.003. PMC . PMID 19942437.
- Kerepesi, Csaba; Grolmusz, Vince (2016). "Giant Viruses of the Kutch Desert". Archives of Virology. 161 (3): 721–724. doi:10.1007/s00705-015-2720-8. PMID 26666442.
- Kerepesi, Csaba; Grolmusz, Vince (2017). "The "Giant Virus Finder" Discovers an Abundance of Giant Viruses in the Antarctic Dry Valleys". Archives of Virology. doi:10.1007/s00705-017-3286-4.
- Zimmer, Carl (13 July 2010). "How Microbes Defend and Define Us". New York Times. Retrieved 29 December 2011.
- Nelson KE and White BA (2010). "Metagenomics and Its Applications to the Study of the Human Microbiome". Metagenomics: Theory, Methods and Applications. Caister Academic Press. ISBN 978-1-904455-54-7.
- Qin, Junjie; Ruiqiang Li; Jeroen Raes; Manimozhiyan Arumugam; Kristoffer Solvesten Burgdorf (March 2010). "A human gut microbial gene catalogue established by metagenomic sequencing". Nature. 464 (7285): 59–65. doi:10.1038/nature08821. PMC . PMID 20203603.
- Li, Luen-Luen; Sean R McCorkle; Sebastien Monchy; Safiyh Taghavi; Daniel van der Lelie (18 May 2009). "Bioprospecting metagenomes: glycosyl hydrolases for converting biomass". Biotechnology for Biofuels. 2: 10. doi:10.1186/1754-6834-2-10. ISSN 1754-6834.
- Jaenicke, Sebastian; Christina Ander; Thomas Bekel; Regina Bisdorf; Marcus Dröge; Karl-Heinz Gartemann; Sebastian Jünemann; Olaf Kaiser; Lutz Krause; Felix Tille; Martha Zakrzewski; Alfred Pühler; Andreas Schlüter; Alexander Goesmann (26 January 2011). Aziz, Ramy K, ed. "Comparative and Joint Analysis of Two Metagenomic Datasets from a Biogas Fermenter Obtained by 454-Pyrosequencing". PLoS ONE. 6 (1): e14519. Bibcode:2011PLoSO...614519J. doi:10.1371/journal.pone.0014519. PMC . PMID 21297863.
- Suen, Garret; Jarrod J Scott; Frank O Aylward; Sandra M Adams; Susannah G Tringe; Adrián A Pinto-Tomás; Clifton E Foster; Markus Pauly; Paul J Weimer; Kerrie W Barry; Lynne A Goodwin; Pascal Bouffard; Lewyn Li; Jolene Osterberger; Timothy T Harkins; Steven C Slater; Timothy J Donohue; Cameron R Currie (September 2010). Sonnenburg, Justin, ed. "An insect herbivore microbiome with high plant biomass-degrading capacity". PLoS Genetics. 6 (9): e1001129. doi:10.1371/journal.pgen.1001129. ISSN 1553-7404. PMC . PMID 20885794.
- George I; et al. (2010). "Application of Metagenomics to Bioremediation". Metagenomics: Theory, Methods and Applications. Caister Academic Press. ISBN 978-1-904455-54-7.
- Committee on Metagenomics: Challenges and Functional Applications, National Research Council (2007). Understanding Our Microbial Planet: The New Science of Metagenomics (PDF). The National Academies Press.
- Simon, C.; Daniel, R. (2009). "Achievements and new knowledge unraveled by metagenomic approaches". Applied Microbiology and Biotechnology. 85 (2): 265–276. doi:10.1007/s00253-009-2233-z. PMC . PMID 19760178.
- Wong D (2010). "Applications of Metagenomics for Industrial Bioproducts". Metagenomics: Theory, Methods and Applications. Caister Academic Press. ISBN 978-1-904455-54-7.
- Schloss, Patrick D; Jo Handelsman (June 2003). "Biotechnological prospects from metagenomics" (PDF). Current Opinion in Biotechnology. 14 (3): 303–310. doi:10.1016/S0958-1669(03)00067-3. ISSN 0958-1669. PMID 12849784. Retrieved 3 January 2012.
- Kakirde, Kavita S.; Larissa C. Parsley; Mark R. Liles (1 November 2010). "Size Does Matter: Application-driven Approaches for Soil Metagenomics". Soil biology & biochemistry. 42 (11): 1911–1923. doi:10.1016/j.soilbio.2010.07.021. ISSN 0038-0717.
- Parachin, Nádia Skorupa; Marie F Gorwa-Grauslund (2011). "Isolation of xylose isomerases by sequence- and function-based screening from a soil metagenomic library". Biotechnology for Biofuels. 4 (1): 9. doi:10.1186/1754-6834-4-9. ISSN 1754-6834. Retrieved 3 January 2012.
- Jansson, Janet (2011). "Towards "Tera-Terra": Terabase Sequencing of Terrestrial Metagenomes Print E-mail". Microbe. 6 (7). p. 309.
- Vogel, T. M.; Simonet, P.; Jansson, J. K.; Hirsch, P. R.; Tiedje, J. M.; Van Elsas, J. D.; Bailey, M. J.; Nalin, R.; Philippot, L. (2009). "TerraGenome: A consortium for the sequencing of a soil metagenome". Nature Reviews Microbiology. 7 (4): 252. doi:10.1038/nrmicro2119.
- "TerraGenome Homepage". TerraGenome international sequencing consortium. Retrieved 30 December 2011.
- Charles T (2010). "The Potential for Investigation of Plant-microbe Interactions Using Metagenomics Methods". Metagenomics: Theory, Methods and Applications. Caister Academic Press. ISBN 978-1-904455-54-7.
- Raes, J.; Letunic, I.; Yamada, T.; Jensen, L. J.; Bork, P. (2011). "Toward molecular trait-based ecology through integration of biogeochemical, geographical and metagenomic data". Molecular Systems Biology. 7: 473. doi:10.1038/msb.2011.6. PMC . PMID 21407210.
- Lavery, T. J.; Roudnew, B.; Seymour, J.; Mitchell, J. G.; Jeffries, T. (2012). Steinke, Dirk, ed. "High Nutrient Transport and Cycling Potential Revealed in the Microbial Metagenome of Australian Sea Lion (Neophoca cinerea) Faeces". PLoS ONE. 7 (5): e36478. doi:10.1371/journal.pone.0036478. PMC . PMID 22606263.
- "What's Swimming In The River? Just Look For DNA". NPR.org. 24 July 2013. Retrieved 10 October 2014.
- Focus on Metagenomics at Nature Reviews Microbiology journal website
- The “Critical Assessment of Metagenome Interpretation” (CAMI) initiative to evaluate methods in metagenomics
- Metagenomics Sequencing for microscopic life Research