Methylaspartate ammonia-lyase

From Wikipedia, the free encyclopedia
Jump to: navigation, search
methylaspartate ammonia-lyase
1kko.png
X-ray structure of methylaspartate ammonia lyase. PDB entry 1kko[1]
Identifiers
EC number 4.3.1.2
CAS number 9033-26-5
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum
Gene Ontology AmiGO / EGO

In enzymology, a methylaspartate ammonia-lyase (EC 4.3.1.2) is an enzyme that catalyzes the chemical reaction

L-threo-3-methylaspartate mesaconate + NH3

Hence, this enzyme has one substrate, L-threo-3-methylaspartate, and two products, mesaconate and NH3.

This enzyme belongs to the family of lyases, specifically ammonia lyases, which cleave carbon-nitrogen bonds. The systematic name of this enzyme class is L-threo-3-methylaspartate ammonia-lyase (mesaconate-forming). Other names in common use include beta-methylaspartase, 3-methylaspartase, and L-threo-3-methylaspartate ammonia-lyase. This enzyme participates in c5-branched dibasic acid metabolism and nitrogen metabolism. It employs one cofactor, cobamide.

Structural studies[edit]

Several structures of this enzyme have been deposited in the Protein Data Bank (linked in the infobox) which show it possesses a TIM barrel domain.

References[edit]

  1. ^ Levy, C. W.; Buckley, P. A.; Sedelnikova, S.; Kato, Y.; Asano, Y.; Rice, D. W.; Baker, P. J. (2002). "Insights into Enzyme Evolution Revealed by the Structure of Methylaspartate Ammonia Lyase". Structure. 10 (1): 105–13. PMID 11796115. doi:10.1016/S0969-2126(01)00696-7. 
  • BARKER HA, SMYTH RD, WAWSZKIEWICZ EJ, LEE MN, WILSON RM (1958). "Enzymic preparation and characterization of an alpha-L-beta-methylaspartic acid". Arch. Biochem. Biophys. 78 (2): 468–76. PMID 13618029. doi:10.1016/0003-9861(58)90371-0. 
  • Bright HJ; Ingraham LL (1960). "The preparation of crystalline beta-methylaspartase". Biochim. Biophys. Acta. 44: 586–588. doi:10.1016/0006-3002(60)91612-7.